C57BL/6NCya-Whammem1flox/Cya
Common Name:
Whamm-flox
Product ID:
S-CKO-17732
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Whamm-flox
Strain ID
CKOCMP-434204-Whamm-B6N-VB
Gene Name
Product ID
S-CKO-17732
Gene Alias
Whdc1; mKIAA1971
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Whammem1flox/Cya mice (Catalog S-CKO-17732) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000165460
NCBI RefSeq
NM_001004185
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
WHAMM, short for WAS Protein Homolog Associated with Actin, Golgi Membranes, and Microtubules, is an actin nucleation promoting factor (NPF) belonging to the Wiskott-Aldrich syndrome protein (WASP) family. It associates with membranes and microtubules and is involved in important cellular processes such as autophagic lysosome reformation (ALR), autophagosome membrane remodeling, and kidney nutrient reabsorption [1,2,5]. It also plays a role in oocyte maturation and spindle formation in mammalian cells [3].
In male WHAMM knockout mice, there is impaired kidney reabsorption, as evidenced by elevated excretion of albumin, glucose, phosphate, and amino acids, along with structural abnormalities in the kidney proximal tubule. In kidney tissue, loss of WHAMM leads to accumulation of lipidated autophagosomal membrane protein LC3, indicating autophagy alteration. In cells, WHAMM and its binding partner Arp2/3 complex control autophagic membrane closure and cargo receptor recruitment [2,4]. In maturing mouse oocytes, knockdown of WHAMM impairs spindle formation, spindle actin polymerization, chromosome alignment, and can cause chromosomal aneuploidy and abnormal, asymmetric division [3].
In conclusion, WHAMM is essential for multiple biological functions, especially in processes related to autophagy and cytoskeletal regulation. The use of WHAMM knockout mouse models has revealed its significance in kidney function and oocyte maturation, which may have implications for understanding related diseases such as kidney disorders and potential fertility-related issues.
References:
1. Dai, Anbang, Yu, Li, Wang, Hong-Wei. 2019. WHAMM initiates autolysosome tubulation by promoting actin polymerization on autolysosomes. In Nature communications, 10, 3699. doi:10.1038/s41467-019-11694-9. https://pubmed.ncbi.nlm.nih.gov/31420534/
2. Coulter, Alyssa M, Cortés, Valerie, Theodore, Corey J, Korstanje, Ron, Campellone, Kenneth G. 2024. WHAMM functions in kidney reabsorption and polymerizes actin to promote autophagosomal membrane closure and cargo sequestration. In Molecular biology of the cell, 35, ar80. doi:10.1091/mbc.E24-01-0025. https://pubmed.ncbi.nlm.nih.gov/38598293/
3. Jo, Yu-Jin, Kwon, Jeongwoo, Jin, Zhe-Long, Kim, Ji-Su, Kim, Nam-Hyung. 2021. WHAMM is essential for spindle formation and spindle actin polymerization in maturing mouse oocytes. In Cell cycle (Georgetown, Tex.), 20, 225-235. doi:10.1080/15384101.2020.1867791. https://pubmed.ncbi.nlm.nih.gov/33397186/
4. Coulter, Alyssa M, Cortés, Valerie, Theodore, Corey J, Korstanje, Ron, Campellone, Kenneth G. 2024. WHAMM functions in kidney reabsorption and polymerizes actin to promote autophagosomal membrane closure and cargo sequestration. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.22.576497. https://pubmed.ncbi.nlm.nih.gov/38328079/
5. Kast, David J, Dominguez, Roberto. . WHAMM links actin assembly via the Arp2/3 complex to autophagy. In Autophagy, 11, 1702-4. doi:10.1080/15548627.2015.1073434. https://pubmed.ncbi.nlm.nih.gov/26291929/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen