C57BL/6JCya-Zfyve1em1flox/Cya
Common Name:
Zfyve1-flox
Product ID:
S-CKO-17734
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfyve1-flox
Strain ID
CKOCMP-217695-Zfyve1-B6J-VC
Gene Name
Product ID
S-CKO-17734
Gene Alias
DFCP1; G630053K23; TAFF1; ZNFN2A1; mKIAA1589
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfyve1em1flox/Cya mice (Catalog S-CKO-17734) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048319
NCBI RefSeq
NM_183154
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
ZFYVE1, also known as DFCP1, is a zinc-finger FYVE domain-containing protein. It is involved in autophagy-related processes, innate immune responses, and may play a role in maintaining tissue homeostasis. In autophagy, it is associated with the biogenesis of autophagosomes, and in the innate immune system, it participates in regulating antiviral responses and TLR3-mediated signaling [2,3,4,5,6].
In terms of functional studies using gene knockout models, Zfyve1-deficiency promoted MDA5-but not RIG-I-mediated transcription of downstream antiviral genes. Zfyve1 -/- mice were significantly protected from lethality induced by encephalomyocarditis virus (EMCV) sensed by MDA5, while Zfyve1 -/- and Zfyve1+/+ mice had comparable responses to vesicular stomatitis virus (VSV) sensed by RIG-I. Mechanistically, ZFYVE1 interacted with MDA5, bound to viral RNA, and decreased the ligand binding and oligomerization of MDA5 [1]. Also, Zfyve1 -/- mice were less susceptible than wild-type mice to inflammatory death induced by the synthetic TLR3 ligand poly(I:C) but not LPS, as ZFYVE1 promoted the transcription of downstream antiviral genes upon poly(I:C) stimulation and was associated with TLR3, increasing its binding affinity to poly(I:C) [6].
In conclusion, ZFYVE1 serves as a crucial regulator in both autophagy and innate immune responses. Gene knockout mouse models have revealed its specific roles in antiviral innate immunity, especially in differentiating between MDA5-and RIG-I-mediated responses, as well as in TLR3-mediated signaling. Understanding ZFYVE1's functions can provide insights into the mechanisms of antiviral defense and inflammatory responses, potentially contributing to the development of strategies for treating related diseases.
References:
1. Zhong, Xuan, Feng, Lu, Zang, Ru, Yang, Qing, Shu, Hong-Bing. 2020. ZFYVE1 negatively regulates MDA5- but not RIG-I-mediated innate antiviral response. In PLoS pathogens, 16, e1008457. doi:10.1371/journal.ppat.1008457. https://pubmed.ncbi.nlm.nih.gov/32251420/
2. Foerster, Elisabeth G, Mukherjee, Tapas, Cabral-Fernandes, Liliane, Girardin, Stephen E, Philpott, Dana J. 2021. How autophagy controls the intestinal epithelial barrier. In Autophagy, 18, 86-103. doi:10.1080/15548627.2021.1909406. https://pubmed.ncbi.nlm.nih.gov/33906557/
3. Nähse, Viola, Schink, Kay O, Stenmark, Harald. 2023. ATPase-regulated autophagosome biogenesis. In Autophagy, 20, 218-219. doi:10.1080/15548627.2023.2255967. https://pubmed.ncbi.nlm.nih.gov/37722386/
4. Devis-Jauregui, Laura, Eritja, Núria, Davis, Meredith Leigh, Matias-Guiu, Xavier, Llobet-Navàs, David. 2020. Autophagy in the physiological endometrium and cancer. In Autophagy, 17, 1077-1095. doi:10.1080/15548627.2020.1752548. https://pubmed.ncbi.nlm.nih.gov/32401642/
5. Xu, Ye, Shen, Jun, Ran, Zhihua. 2019. Emerging views of mitophagy in immunity and autoimmune diseases. In Autophagy, 16, 3-17. doi:10.1080/15548627.2019.1603547. https://pubmed.ncbi.nlm.nih.gov/30951392/
6. Zhong, Xuan, Feng, Lu, Xu, Wen-Hua, Lei, Cao-Qi, Shu, Hong-Bing. 2019. The zinc-finger protein ZFYVE1 modulates TLR3-mediated signaling by facilitating TLR3 ligand binding. In Cellular & molecular immunology, 17, 741-752. doi:10.1038/s41423-019-0265-6. https://pubmed.ncbi.nlm.nih.gov/31388100/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen