C57BL/6JCya-Tecpr1em1flox/Cya
Common Name:
Tecpr1-flox
Product ID:
S-CKO-17745
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tecpr1-flox
Strain ID
CKOCMP-70381-Tecpr1-B6J-VC
Gene Name
Product ID
S-CKO-17745
Gene Alias
2210010N04Rik; mKIAA1358
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tecpr1em1flox/Cya mice (Catalog S-CKO-17745) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000085701
NCBI RefSeq
NM_027410
Target Region
Exon 9
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Tecpr1, or tectonin beta-propeller repeat containing 1, is a crucial protein involved in multiple autophagy-related pathways. Autophagy is a degradative mechanism in cells, and Tecpr1 plays a key role in non-canonical autophagy, also called conjugation of ATG8 to single membranes (CASM). It helps label damaged intracellular compartments with ATG8 family proteins, signaling danger from pathogens or toxic compounds [1,2,3,4].
In KO mouse models, Tecpr1-deficient MEFs were defective for selective autophagy, supporting increased intracellular multiplication of Shigella. Also, depolarized mitochondria and misfolded protein aggregates accumulated in these cells, indicating its importance in targeting bacterial pathogens for selective autophagy [5]. In P301S-tau transgenic mice, overexpression of Tecpr1 improved cognitive function via activation of autophagy in the early and late process [6].
In conclusion, Tecpr1 is essential for non-canonical autophagy and selective autophagy processes. Studies using KO mouse models have revealed its significance in host innate immune response against bacterial pathogens and in improving cognitive function in a tau-related disease model, providing insights into potential therapeutic targets for related diseases.
References:
1. Kaur, Namrita, de la Ballina, Laura Rodriguez, Haukaas, Håvard Styrkestad, Carlsson, Sven R, Lystad, Alf Håkon. 2023. TECPR1 is activated by damage-induced sphingomyelin exposure to mediate noncanonical autophagy. In The EMBO journal, 42, e113105. doi:10.15252/embj.2022113105. https://pubmed.ncbi.nlm.nih.gov/37409525/
2. Boyle, Keith B, Ellison, Cara J, Elliott, Paul R, Martens, Sascha, Randow, Felix. 2023. TECPR1 conjugates LC3 to damaged endomembranes upon detection of sphingomyelin exposure. In The EMBO journal, 42, e113012. doi:10.15252/embj.2022113012. https://pubmed.ncbi.nlm.nih.gov/37409490/
3. Kaur, Namrita, Carlsson, Sven R, Lystad, Alf Håkon. 2023. The separate axes of TECPR1 and ATG16L1 in CASM. In Autophagy, 20, 214-215. doi:10.1080/15548627.2023.2255462. https://pubmed.ncbi.nlm.nih.gov/37676042/
4. Figueras-Novoa, Carmen, Timimi, Lewis, Marcassa, Elena, Ulferts, Rachel, Beale, Rupert. 2024. Conjugation of ATG8s to single membranes at a glance. In Journal of cell science, 137, . doi:10.1242/jcs.261031. https://pubmed.ncbi.nlm.nih.gov/39145464/
5. Ogawa, Michinaga, Yoshikawa, Yuko, Kobayashi, Taira, Reichhart, Jean-Marc, Sasakawa, Chihiro. . A Tecpr1-dependent selective autophagy pathway targets bacterial pathogens. In Cell host & microbe, 9, 376-89. doi:10.1016/j.chom.2011.04.010. https://pubmed.ncbi.nlm.nih.gov/21575909/
6. Li, Ting, Liu, Ruijuan, He, Ye, Li, Xiao, Liu, Gong-Ping. 2024. Overexpression of TECPR1 improved cognitive function of P301S-tau mice via activation of autophagy in the early and late process. In Aging cell, 24, e14404. doi:10.1111/acel.14404. https://pubmed.ncbi.nlm.nih.gov/39511758/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen