C57BL/6JCya-Elovl1em1flox/Cya
Common Name:
Elovl1-flox
Product ID:
S-CKO-17750
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Elovl1-flox
Strain ID
CKOCMP-54325-Elovl1-B6J-VB
Gene Name
Product ID
S-CKO-17750
Gene Alias
Ssc1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Elovl1em1flox/Cya mice (Catalog S-CKO-17750) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000006557
NCBI RefSeq
NM_019422
Target Region
Exon 2~8
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Elovl1, or elongation of very long-chain fatty acids protein 1, is an enzyme that catalyzes the rate-limiting step in the synthesis of very long-chain fatty acids (VLCFAs), specifically elongating saturated and monounsaturated C22-C26-VLCFAs. It is involved in lipid metabolism and is associated with pathways such as PI3K-AKT-mTOR [2]. Its activity is crucial for the production of C24 sphingolipids [7].
Astrocyte-specific knockout of Elovl1 mitigates astrocyte-mediated toxicity in vitro and in an acute axonal injury model in vivo, suggesting it plays a role in astrocyte-induced cell death via saturated lipids [1]. In hepatocellular carcinoma, silencing Elovl1 in cell lines inhibits cell growth, promotes apoptosis, and decreases metastasis, and low expression in a xenograft model inhibits tumor growth, indicating its role in promoting tumor growth through the PI3K-AKT-mTOR signaling pathway [2]. In adrenoleukodystrophy (ALD), inhibitors of Elovl1 reduce C26:0 VLCFA synthesis in patient cells and animal models [3,4,5]. In CD8+ T cells, inactivation of Elovl1 combined with anti-PD-1 shows therapeutic efficacy in resistant tumors, enhancing T cell fitness [6]. A dominant mutation in Elovl1 abrogates its enzymatic activity, causing a neuro-ichthyotic disorder [8].
In conclusion, Elovl1 is essential for VLCFA synthesis and C24 sphingolipid production. Gene knockout and knockdown models in various systems have revealed its roles in diseases like neurodegeneration, cancer, ALD, and in enhancing T cell fitness in tumors. These models contribute significantly to understanding its function in specific disease conditions, providing potential therapeutic targets for these diseases.
References:
1. Guttenplan, Kevin A, Weigel, Maya K, Prakash, Priya, Liddelow, Shane A, Barres, Ben A. 2021. Neurotoxic reactive astrocytes induce cell death via saturated lipids. In Nature, 599, 102-107. doi:10.1038/s41586-021-03960-y. https://pubmed.ncbi.nlm.nih.gov/34616039/
2. Qin, Liang, Song, Cheng-Ze, Yuan, Fa-Yang, Ma, Yi-Fei, Chen, Zi-Li. 2024. ELOVL1 is upregulated and promotes tumor growth in hepatocellular carcinoma through regulating PI3K-AKT-mTOR signaling. In Heliyon, 10, e34961. doi:10.1016/j.heliyon.2024.e34961. https://pubmed.ncbi.nlm.nih.gov/39144963/
3. Siddiqui, Arif Jamal, Kumar, Vikash, Jahan, Sadaf, Snoussi, Mejdi, Adnan, Mohd. 2023. Computational insight into structural basis of human ELOVL1 inhibition. In Computers in biology and medicine, 157, 106786. doi:10.1016/j.compbiomed.2023.106786. https://pubmed.ncbi.nlm.nih.gov/36924735/
4. Come, Jon H, Senter, Timothy J, Clark, Michael P, Charifson, Paul S, Magavi, Sanjay S. 2021. Discovery and Optimization of Pyrazole Amides as Inhibitors of ELOVL1. In Journal of medicinal chemistry, 64, 17753-17776. doi:10.1021/acs.jmedchem.1c00944. https://pubmed.ncbi.nlm.nih.gov/34748351/
5. Boyd, Michael J, Collier, Philip N, Clark, Michael P, Charifson, Paul S, Magavi, Sanjay Shivayogi. 2021. Discovery of Novel, Orally Bioavailable Pyrimidine Ether-Based Inhibitors of ELOVL1. In Journal of medicinal chemistry, 64, 17777-17794. doi:10.1021/acs.jmedchem.1c00948. https://pubmed.ncbi.nlm.nih.gov/34871500/
6. Pretto, Samantha, Yu, Qian, Bourdely, Pierre, Di Matteo, Mario, Mazzone, Massimiliano. 2025. A functional single-cell metabolic survey identifies Elovl1 as a target to enhance CD8+ T cell fitness in solid tumours. In Nature metabolism, 7, 508-530. doi:10.1038/s42255-025-01233-w. https://pubmed.ncbi.nlm.nih.gov/40065102/
7. Ohno, Yusuke, Suto, Shota, Yamanaka, Masao, Sassa, Takayuki, Kihara, Akio. 2010. ELOVL1 production of C24 acyl-CoAs is linked to C24 sphingolipid synthesis. In Proceedings of the National Academy of Sciences of the United States of America, 107, 18439-44. doi:10.1073/pnas.1005572107. https://pubmed.ncbi.nlm.nih.gov/20937905/
8. Mueller, Noomi, Sassa, Takayuki, Morales-Gonzalez, Susanne, Kihara, Akio, Schuelke, Markus. 2018. De novo mutation in ELOVL1 causes ichthyosis, acanthosis nigricans, hypomyelination, spastic paraplegia, high frequency deafness and optic atrophy. In Journal of medical genetics, 56, 164-175. doi:10.1136/jmedgenet-2018-105711. https://pubmed.ncbi.nlm.nih.gov/30487246/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen