C57BL/6JCya-Ifitm1em1flox/Cya
Common Name
Ifitm1-flox
Product ID
S-CKO-17757
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-68713-Ifitm1-B6J-VA
Status
When using this mouse strain in a publication, please cite “Ifitm1-flox Mouse (Catalog S-CKO-17757) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ifitm1-flox
Strain ID
CKOCMP-68713-Ifitm1-B6J-VA
Gene Name
Product ID
S-CKO-17757
Gene Alias
Mil2, Mil-2, DSPA2a, 1110036C17Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000106042
NCBI RefSeq
NM_026820
Target Region
Exon 3
Size of Effective Region
~1.3 kb
Overview of Gene Research
Ifitm1, short for Interferon (IFN)-induced transmembrane protein 1, is a member of the IFN-induced transmembrane protein family. It plays a crucial role in the innate immune response triggered by viral infection, with functions including conferring cellular resistance to many viruses and being involved in intracellular cholesterol transport [2,6,5]. It is also associated with pathways like the STING-TBK1-IRF3 signaling pathway [3].
In cancer, overexpression of Ifitm1 promotes tumor cell proliferation, invasion, metastasis, angiogenesis, and therapeutic resistance in various tumor types such as gallbladder carcinoma, esophageal adenocarcinoma, colorectal cancer, and gastric cancer, making it an independent prognostic biomarker [1]. In colorectal cancer, Apc mutation induces Ifitm1 expression, and high Ifitm1 levels in cells reduce fibroblast extracellular vesicle (EV) uptake, with functional implications for cell proliferation [4]. In lung adenocarcinoma, high expression of Ifitm1, along with FOXM1 and PLK1, is inversely correlated with patient survival rate, as Ifitm1 enhances FoxM1-mediated signaling [3]. In brain metastasis from lung cancer, loss of Ifitm1 promotes brain colonization, while high Ifitm1 expression in incipient metastatic cells activates microglia and CD8+ T cells to eliminate cancer cells, and it also enhances the efficacy of PD-1 blockade [9].
In viral infections, overexpression of Ifitm1 suppresses porcine epidemic diarrhea virus (PEDV) proliferation [2]. It also enhances non-enveloped viral (Aichi virus) RNA replication by facilitating cholesterol transport to the Golgi [5]. In SARS-CoV-2 infection, IFITM1 methylation is higher in severe and critical patients, potentially suppressing the immune system and promoting viral replication [7], and IFITM1 inhibits Spike-mediated fusion of SARS-CoV-2-infected cells [8].
In conclusion, Ifitm1 has diverse functions in both antiviral immune responses and cancer-related processes. Model-based research, especially in the context of cancer and viral infections, has revealed its significance in promoting or suppressing disease progression, highlighting its potential as a therapeutic target in these disease areas.
References：
1. Liang, Renba, Li, Xinxiao, Zhu, Xiaodong. . Deciphering the Roles of IFITM1 in Tumors. In Molecular diagnosis & therapy, 24, 433-441. doi:10.1007/s40291-020-00469-4. https://pubmed.ncbi.nlm.nih.gov/32394410/
2. Cheng, Jiahao, He, Jiayi, Feng, Simeng, Wang, Aibing, Yuan, Xiaomin. 2024. IFITM1 is a host restriction factor that inhibits porcine epidemic diarrhea virus infection. In Journal of nanobiotechnology, 22, 677. doi:10.1186/s12951-024-02884-9. https://pubmed.ncbi.nlm.nih.gov/39501328/
3. Xu, Rong, Lee, Young-Joo, Kim, Chang-Hyeon, Kim, Jung-Hyun, Yim, Hyungshin. 2023. Invasive FoxM1 phosphorylated by PLK1 induces the polarization of tumor-associated macrophages to promote immune escape and metastasis, amplified by IFITM1. In Journal of experimental & clinical cancer research : CR, 42, 302. doi:10.1186/s13046-023-02872-1. https://pubmed.ncbi.nlm.nih.gov/37968723/
4. Kelemen, Andrea, Carmi, Idan, Oszvald, Ádám, Buzás, Edit I, Wiener, Zoltán. 2021. IFITM1 expression determines extracellular vesicle uptake in colorectal cancer. In Cellular and molecular life sciences : CMLS, 78, 7009-7024. doi:10.1007/s00018-021-03949-w. https://pubmed.ncbi.nlm.nih.gov/34609520/
5. Ishikawa-Sasaki, Kumiko, Murata, Takayuki, Sasaki, Jun. 2023. IFITM1 enhances nonenveloped viral RNA replication by facilitating cholesterol transport to the Golgi. In PLoS pathogens, 19, e1011383. doi:10.1371/journal.ppat.1011383. https://pubmed.ncbi.nlm.nih.gov/37252940/
6. Yánez, Diana C, Ross, Susan, Crompton, Tessa. 2019. The IFITM protein family in adaptive immunity. In Immunology, 159, 365-372. doi:10.1111/imm.13163. https://pubmed.ncbi.nlm.nih.gov/31792954/
7. Arefinia, Nasir, Yaghoubi, Ramin, Ramezani, Amin, Zadeh, Ali Mohammad Arab, Sarvari, Jamal. . Association of IFITM1 Promoter Methylation with Severity of SARS-CoV-2 Infection. In Clinical laboratory, 69, . doi:10.7754/Clin.Lab.2022.220622. https://pubmed.ncbi.nlm.nih.gov/37057950/
8. Buchrieser, Julian, Dufloo, Jérémy, Hubert, Mathieu, Bruel, Timothée, Schwartz, Olivier. 2020. Syncytia formation by SARS-CoV-2-infected cells. In The EMBO journal, 39, e106267. doi:10.15252/embj.2020106267. https://pubmed.ncbi.nlm.nih.gov/33051876/
9. She, Xiaofei, Shen, Shijun, Chen, Guang, Qin, Huanlong, Gao, Hua. 2023. Immune surveillance of brain metastatic cancer cells is mediated by IFITM1. In The EMBO journal, 42, e111112. doi:10.15252/embj.2022111112. https://pubmed.ncbi.nlm.nih.gov/36799040/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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