C57BL/6JCya-Usp9xem1flox/Cya
Common Name:
Usp9x-flox
Product ID:
S-CKO-17758
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Usp9x-flox
Strain ID
CKOCMP-22284-Usp9x-B6J-VB
Gene Name
Product ID
S-CKO-17758
Gene Alias
5730589N07Rik; Dffrx; FAF-X; Fafl
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Usp9xem1flox/Cya mice (Catalog S-CKO-17758) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000089302
NCBI RefSeq
NM_009481
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Usp9x, or ubiquitin-specific peptidase 9 X-linked, is a deubiquitinase that plays a crucial role in various biological processes. It promotes the deubiquitination and stabilization of diverse substrates, thereby influencing multiple physiological and pathological pathways [4].
In macrophages, disruption of Usp9x promotes foam cell formation and atherosclerosis. Macrophage Usp9x-deficient mice showed increased macrophage infiltration, lipid deposition, and necrotic core content in atherosclerotic lesions compared to control Apoe-/-mice. Mechanistically, Usp9x removes the K63 polyubiquitin chain of SR-A1 at the K27 site, and inhibition of Usp9x increases SR-A1 cell-surface internalization after ox-LDL binding [1].
In glioblastoma stem cells, depletion of Usp9x downregulates ALDH1A3, resulting in loss of self-renewal and tumorigenic capacity. The USP9X inhibitor WP1130 shows efficacy in MES GSC-derived orthotopic xenograft models [2].
In lung cancer, depletion of Usp9X impairs TGF-β2/Smad signaling and radioresistance by destabilizing KDM4C [3].
In laryngeal cancer, knockdown of Usp9X led to decreased proliferation, migration, and invasion of FaDu cells, and increased apoptosis [6].
In liver fibrosis, USP9X-mediated NRP1 deubiquitination promotes liver fibrosis by activating hepatic stellate cells [5].
In P301S mice, inhibition of USP9X with WP1130 promoted autophagy and accelerated tau degradation, highlighting it as a potential target for tauopathies [7].
In sepsis-induced acute kidney injury, interference with Usp9x alleviated the inflammatory response and apoptosis of renal tubular epithelial cells [8].
In conclusion, Usp9x is a key deubiquitinase involved in multiple biological processes and diseases. Gene knockout or conditional knockout mouse models, such as macrophage Usp9x-deficient mice, have been instrumental in revealing its role in atherosclerosis, cancer development, fibrosis, neurodegenerative diseases, and kidney injury. These findings provide potential therapeutic targets for these disease areas.
References:
1. Wang, Biqing, Tang, Xuening, Yao, Liu, Jiang, Hongfeng, Ai, Ding. . Disruption of USP9X in macrophages promotes foam cell formation and atherosclerosis. In The Journal of clinical investigation, 132, . doi:10.1172/JCI154217. https://pubmed.ncbi.nlm.nih.gov/35389885/
2. Chen, Zhengxin, Wang, Hong-Wei, Wang, Shuai, You, Yongping, Wang, Huibo. 2019. USP9X deubiquitinates ALDH1A3 and maintains mesenchymal identity in glioblastoma stem cells. In The Journal of clinical investigation, 129, 2043-2055. doi:10.1172/JCI126414. https://pubmed.ncbi.nlm.nih.gov/30958800/
3. Jie, Xiaohua, Fong, William Pat, Zhou, Rui, Wu, Gang, Xu, Shuangbing. 2021. USP9X-mediated KDM4C deubiquitination promotes lung cancer radioresistance by epigenetically inducing TGF-β2 transcription. In Cell death and differentiation, 28, 2095-2111. doi:10.1038/s41418-021-00740-z. https://pubmed.ncbi.nlm.nih.gov/33558705/
4. Meng, Yimei, Hong, Chaojin, Yang, Sifu, Yang, Liu, Huang, Yumei. 2023. Roles of USP9X in cellular functions and tumorigenesis (Review). In Oncology letters, 26, 506. doi:10.3892/ol.2023.14093. https://pubmed.ncbi.nlm.nih.gov/37920433/
5. Zhao, Jinqiu, Bai, Jie, Peng, Fengling, Li, Yongguo, Zhong, Li. 2023. USP9X-mediated NRP1 deubiquitination promotes liver fibrosis by activating hepatic stellate cells. In Cell death & disease, 14, 40. doi:10.1038/s41419-022-05527-9. https://pubmed.ncbi.nlm.nih.gov/36653359/
6. Wan, Yu-Feng, Zhang, Chen-Yu, Cheng, Xiao-Wen, Zhu, Hua-Qing, Liu, Ye-Hai. 2023. USP9X expression is functionally related to laryngeal cancer. In Journal of Cancer, 14, 591-599. doi:10.7150/jca.81054. https://pubmed.ncbi.nlm.nih.gov/37057289/
7. Zheng, Siyi, Zhu, Jiahui, Wang, Cailin, Ma, Rong, Li, Gang. 2024. USP9X-mediated deubiquitination of Raptor contributes to autophagy impairment and memory deficits in P301S mice. In Cell communication and signaling : CCS, 22, 516. doi:10.1186/s12964-024-01872-8. https://pubmed.ncbi.nlm.nih.gov/39449082/
8. Gong, Shuhao, Xiong, Huawei, Lei, Yingchao, Cao, Chunshui, Wang, Ying. 2024. Usp9x contributes to the development of sepsis-induced acute kidney injury by promoting inflammation and apoptosis in renal tubular epithelial cells via activation of the TLR4/nf-κb pathway. In Renal failure, 46, 2361089. doi:10.1080/0886022X.2024.2361089. https://pubmed.ncbi.nlm.nih.gov/38874156/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen