C57BL/6JCya-Snhg18em1flox/Cya
Common Name:
Snhg18-flox
Product ID:
S-CKO-17762
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Snhg18-flox
Strain ID
CKOCMP-100616095-Snhg18-B6J-VA
Gene Name
Product ID
S-CKO-17762
Gene Alias
0610007N19Rik; Snord123
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
15
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Snhg18em1flox/Cya mice (Catalog S-CKO-17762) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000181536
NCBI RefSeq
NR_038186
Target Region
Exon 2
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Snhg18, short for small nucleolar RNA host gene 18, is a long non-coding RNA that has been implicated in a variety of biological processes and diseases. It is involved in cell cycle regulation, cell proliferation, apoptosis, and cell motility. It also participates in pathways such as the ubiquitination-proteasome pathway, and is associated with the regulation of genes like c-Myc, p21, miR-22-3p, miR-211-5p, and BRD4 [1,3,4]. Its role in different diseases makes it an important target for understanding disease mechanisms.
In bladder cancer, overexpression of Snhg18 inhibited cell proliferation both in vitro and in vivo. It achieved this by inhibiting c-Myc expression through the ubiquitination-proteasome pathway, which led to an increase in p21 transcription, causing G0-G1 arrest [1]. In hepatitis B virus-associated hepatocellular carcinoma, downregulated Snhg18 was involved in disease development, and enhancing it might be the mechanism underlying the antitumor effect of oleanolic acid [2]. In non-small cell lung cancer, Snhg18, induced by MKL1, promoted cell growth and metastasis through the miR-211-5p/BRD4 axis [4]. In glioma, Snhg18 promoted cell invasion and migration by disrupting the nucleocytoplasmic transport of α-enolase (ENO1) [5]. In ovarian aging, downregulated Snhg18 in human granulosa cells induced apoptosis via disruption of glycolysis [6]. In gastric cancer, Snhg18, induced by SHP2-mediated ROS/JNK/NFAT4 signaling pathway, promoted cancer growth and metastasis through the miR-211-5p/BRD4 axis in CAR-T cells [7]. In metastatic castration-resistant prostate cancer, Snhg18 was downregulated and enriched to metastasis signatures, being associated with epithelial-mesenchymal transition and cell-matrix adhesion pathways [8]. In glioma, Snhg18 accelerated progression via regulating the miR-338-5p/FOXD1 axis [9]. In multiple myeloma, high expression of Snhg18 was associated with poor prognosis [10].
In conclusion, Snhg18 is a crucial long non-coding RNA involved in multiple biological processes and disease conditions. Through functional studies, mainly in vitro and in some cases in vivo, its role in cell proliferation, apoptosis, and metastasis in various cancers and other diseases has been revealed. Understanding Snhg18 provides insights into disease mechanisms and potential therapeutic targets for these diseases.
References:
1. Ke, Meixia, Sun, Ning, Lin, Zhenni, Lu, Yongyong, Jin, Honglei. 2023. SNHG18 inhibits bladder cancer cell proliferation by increasing p21 transcription through destabilizing c-Myc protein. In Cancer cell international, 23, 48. doi:10.1186/s12935-023-02887-w. https://pubmed.ncbi.nlm.nih.gov/36927398/
2. Song, Wenqi, Zhang, Xuemei, Feng, Lin, Li, Tongtong, Zhang, Pengxia. 2022. Downregulated lncRNA SNHG18 Suppresses the Progression of Hepatitis B Virus-Associated Hepatocellular Carcinoma and Meditates the Antitumor Effect of Oleanolic Acid. In Cancer management and research, 14, 687-695. doi:10.2147/CMAR.S346920. https://pubmed.ncbi.nlm.nih.gov/35221722/
3. Niu, Kaiyuan, Zhang, Chengxin, Yang, Mei, Ge, Shenglin, Xiao, Qingzhong. . Small nucleolar RNA host gene 18 controls vascular smooth muscle cell contractile phenotype and neointimal hyperplasia. In Cardiovascular research, 120, 796-810. doi:10.1093/cvr/cvae055. https://pubmed.ncbi.nlm.nih.gov/38498586/
4. Fan, Huijie, Yuan, Jing, Li, Yaqing, Wang, Xiaofeng, Li, Xingya. 2021. MKL1-induced lncRNA SNHG18 drives the growth and metastasis of non-small cell lung cancer via the miR-211-5p/BRD4 axis. In Cell death & disease, 12, 128. doi:10.1038/s41419-021-03399-z. https://pubmed.ncbi.nlm.nih.gov/33500406/
5. Zheng, Rong, Yao, Qiwei, Li, XiaoBo, Xu, Benhua. 2019. Long Noncoding Ribonucleic Acid SNHG18 Promotes Glioma Cell Motility via Disruption of α-Enolase Nucleocytoplasmic Transport. In Frontiers in genetics, 10, 1140. doi:10.3389/fgene.2019.01140. https://pubmed.ncbi.nlm.nih.gov/31798634/
6. Zhao, Xuehan, Zhao, Feiyan, Yan, Long, Xin, Zhimin, Yang, Xiaokui. 2024. Long non-coding ribonucleic acid SNHG18 induced human granulosa cell apoptosis via disruption of glycolysis in ovarian aging. In Journal of ovarian research, 17, 185. doi:10.1186/s13048-024-01510-4. https://pubmed.ncbi.nlm.nih.gov/39272131/
7. An, Lin, Huo, Yue, Xiao, Na, Su, Shenyong, Wang, Kunjie. 2024. SHP2 mediates the ROS/JNK/NFAT4 signaling pathway in gastric cancer cells prompting lncRNA SNHG18 to drive gastric cancer growth and metastasis via CAR-T cells. In Heliyon, 10, e34008. doi:10.1016/j.heliyon.2024.e34008. https://pubmed.ncbi.nlm.nih.gov/39130478/
8. Ferraz, Rafaella Sousa, Cavalcante, João Vitor Ferreira, Magalhães, Leandro, Ribeiro-Dos-Santos, Ândrea, Dalmolin, Rodrigo Juliani Siqueira. 2023. Revealing metastatic castration-resistant prostate cancer master regulator through lncRNAs-centered regulatory network. In Cancer medicine, 12, 19279-19290. doi:10.1002/cam4.6481. https://pubmed.ncbi.nlm.nih.gov/37644825/
9. Ma, Quanfeng, Yang, Tianhao. . E2F transcription factor 1/small nucleolar RNA host gene 18/microRNA-338-5p/forkhead box D1: an important regulatory axis in glioma progression. In Bioengineered, 13, 418-430. doi:10.1080/21655979.2021.2005990. https://pubmed.ncbi.nlm.nih.gov/34937497/
10. Huang, Ling-Juan, Shen, Ying, Bai, Ju, Hu, Jin-Song, He, Ai-Li. 2019. High Expression Levels of Long Noncoding RNA Small Nucleolar RNA Host Gene 18 and Semaphorin 5A Indicate Poor Prognosis in Multiple Myeloma. In Acta haematologica, 143, 279-288. doi:10.1159/000502404. https://pubmed.ncbi.nlm.nih.gov/31597158/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen