C57BL/6JCya-Rbm8aem1flox/Cya
Common Name:
Rbm8a-flox
Product ID:
S-CKO-17775
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rbm8a-flox
Strain ID
CKOCMP-60365-Rbm8a-B6J-VB
Gene Name
Product ID
S-CKO-17775
Gene Alias
2310057C03Rik; Rbm8
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbm8aem1flox/Cya mice (Catalog S-CKO-17775) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000196456
NCBI RefSeq
NM_001102407
Target Region
Exon 2~4
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Rbm8a, also known as Y14, is a component of the exon junction complex involved in mRNA splicing, transport, and nonsense-mediated decay. It plays a crucial role in multiple biological processes, such as embryonic development, hematopoiesis, and neurogenesis, and is associated with various pathways like Wnt/PCP, Notch/STAT3, and PI3K-AKT/mTOR [4,2,1,3].
In zebrafish, rbm8a perturbation leads to early hematopoietic defects via attenuated non-canonical Wnt/PCP signaling, resulting in reduced thrombocytes [4]. In mice, Rbm8a haploinsufficiency causes severe microcephaly and defective neurogenesis, disrupting radial glia proliferation and neuronal differentiation [5]. In cancer, knockdown of Rbm8a in breast cancer and glioblastoma cells inhibits cell proliferation, invasion, and promotes apoptosis, and overexpression has the opposite effects [1,2,3].
In conclusion, Rbm8a is essential for normal development and its dysregulation is associated with various diseases. Gene-knockout models in zebrafish and mice have revealed its key roles in hematopoiesis, cortical development, and cancer progression, providing valuable insights into disease mechanisms and potential therapeutic targets.
References：
1. Li, Fang, Wang, Xiaofei, Zhang, Jing, Tong, Dongdong, Huang, Chen. 2024. RBM8A, a new target of TEAD4, promotes breast cancer progression by regulating IGF1R and IRS-2. In Journal of translational medicine, 22, 823. doi:10.1186/s12967-024-05639-0. https://pubmed.ncbi.nlm.nih.gov/39232805/
2. Lin, Yan, Wei, Lei, Hu, Beiquan, Qian, Zhongrun, Zou, Donghua. 2021. RBM8A Promotes Glioblastoma Growth and Invasion Through the Notch/STAT3 Pathway. In Frontiers in oncology, 11, 736941. doi:10.3389/fonc.2021.736941. https://pubmed.ncbi.nlm.nih.gov/34804926/
3. Song, Tao, Zhang, Huazhou. 2022. RBM8A Depletion Decreases the Cisplatin Resistance and Represses the Proliferation and Metastasis of Breast Cancer Cells via AKT/mTOR Pathway. In The breast journal, 2022, 4576789. doi:10.1155/2022/4576789. https://pubmed.ncbi.nlm.nih.gov/36105365/
4. Kocere, Agnese, Chiavacci, Elena, Soneson, Charlotte, Mosimann, Christian, Burger, Alexa. 2024. Rbm8a deficiency causes hematopoietic defects by modulating Wnt/PCP signaling. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.04.12.536513. https://pubmed.ncbi.nlm.nih.gov/37090609/
5. Mao, Hanqian, Pilaz, Louis-Jan, McMahon, John J, Katsanis, Nicholas, Silver, Debra L. . Rbm8a haploinsufficiency disrupts embryonic cortical development resulting in microcephaly. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 35, 7003-18. doi:10.1523/JNEUROSCI.0018-15.2015. https://pubmed.ncbi.nlm.nih.gov/25948253/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen