C57BL/6NCya-Aregem1flox/Cya
Common Name
Areg-flox
Product ID
S-CKO-17789
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-11839-Areg-B6N-VA
Status
When using this mouse strain in a publication, please cite “Areg-flox Mouse (Catalog S-CKO-17789) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Areg-flox
Strain ID
CKOCMP-11839-Areg-B6N-VA
Gene Name
Product ID
S-CKO-17789
Gene Alias
AR, Mcub, Sdgf
Background
C57BL/6NCya
Gene Full Name
amphiregulin
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 5 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000031325
NCBI Transcript ID
NM_009704
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Overview of Gene Research
Areg, also known as Amphiregulin, is a member of the epidermal growth factor (EGF) family. It functions as an extracellular ligand for the EGF receptor (EGFR) and also has intracellular signaling roles. Areg is involved in multiple biological processes such as cell proliferation, tissue repair, and immune responses, and is associated with pathways like Hippo-YAP, EGFR-Erk/p38 MAPK, and EGFR/ERK/NF-κB [1,2,6]. It plays a crucial role in various physiological and pathological conditions, making genetic models valuable for studying its functions.
In psoriasis, IL-17A promotes keratinocyte proliferation by activating the YAP-AREG axis [1]. In esophageal squamous cell carcinoma, co-culture with cancer-associated fibroblasts upregulates AREG, which promotes cancer progression through the EGFR-Erk/p38 MAPK signaling pathway [2]. In melanoma, nuclear AREG affects a low-proliferative phenotype and contributes to drug resistance [3]. In human granulosa cells, AREG upregulates the secreted protein acidic and rich in cysteine (SPARC) expression, which is related to progesterone production [4]. In non-alcoholic steatohepatitis, Areg-producing regulatory T cells promote liver fibrosis and insulin resistance [5]. In pancreatic cancer, AREG mediates epithelial-mesenchymal transition via the EGFR/ERK/NF-κB signaling pathway, and miR-33a-3p can regulate AREG stability to inhibit pancreatic cancer invasion and metastasis [6,7]. In ovarian hyperstimulation syndrome (OHSS), AREG in granulosa cells is elevated and contributes to increased VEGF expression [8].
In conclusion, Areg has diverse functions in cell proliferation, tissue repair, and disease development. Studies using various models, including those indirectly related to gene knockout concepts by manipulating Areg levels, have revealed its significance in diseases such as psoriasis, cancer, and liver and ovarian diseases. These findings help in understanding the underlying mechanisms and potentially developing targeted therapies for these conditions.
References：
1. Yu, Zengyang, Yu, Qian, Xu, Hui, Guo, Chunyuan, Shi, Yuling. 2022. IL-17A Promotes Psoriasis-Associated Keratinocyte Proliferation through ACT1-Dependent Activation of YAP-AREG Axis. In The Journal of investigative dermatology, 142, 2343-2352. doi:10.1016/j.jid.2022.02.016. https://pubmed.ncbi.nlm.nih.gov/35304250/
2. Nakanishi, Takashi, Koma, Yu-Ichiro, Miyako, Shoji, Yokozaki, Hiroshi, Kakeji, Yoshihiro. 2024. AREG Upregulation in Cancer Cells via Direct Interaction with Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression Through EGFR-Erk/p38 MAPK Signaling. In Cells, 13, . doi:10.3390/cells13201733. https://pubmed.ncbi.nlm.nih.gov/39451251/
3. Seefried, Felix, Haller, Lucia, Fukuda, Shinji, Bosserhoff, Anja Katrin, Kuphal, Silke. 2022. Nuclear AREG affects a low-proliferative phenotype and contributes to drug resistance of melanoma. In International journal of cancer, 151, 2244-2264. doi:10.1002/ijc.34254. https://pubmed.ncbi.nlm.nih.gov/36054710/
4. Dang, Xuan, Fang, Lanlan, Zhang, Qian, Cheng, Jung-Chien, Sun, Ying-Pu. 2022. AREG upregulates secreted protein acidic and rich in cysteine expression in human granulosa cells. In Molecular and cellular endocrinology, 561, 111826. doi:10.1016/j.mce.2022.111826. https://pubmed.ncbi.nlm.nih.gov/36462647/
5. Savage, Thomas M, Fortson, Katherine T, de Los Santos-Alexis, Kenia, Schwabe, Robert F, Arpaia, Nicholas. 2024. Amphiregulin from regulatory T cells promotes liver fibrosis and insulin resistance in non-alcoholic steatohepatitis. In Immunity, 57, 303-318.e6. doi:10.1016/j.immuni.2024.01.009. https://pubmed.ncbi.nlm.nih.gov/38309273/
6. Wang, Li, Wang, Lili, Zhang, Hui, Wu, Huanwen, Liang, Zhiyong. 2020. AREG mediates the epithelial‑mesenchymal transition in pancreatic cancer cells via the EGFR/ERK/NF‑κB signalling pathway. In Oncology reports, 43, 1558-1568. doi:10.3892/or.2020.7523. https://pubmed.ncbi.nlm.nih.gov/32323797/
7. Su, Xiaowen, Lai, Tiantian, Tao, Yue, Mao, Yong, Hu, Hao. 2023. miR-33a-3p regulates METTL3-mediated AREG stability and alters EMT to inhibit pancreatic cancer invasion and metastasis. In Scientific reports, 13, 13587. doi:10.1038/s41598-023-39506-7. https://pubmed.ncbi.nlm.nih.gov/37604948/
8. Fang, Lanlan, Yu, Yiping, Li, Yiran, Zhang, Ruizhe, Sun, Ying-Pu. . Upregulation of AREG, EGFR, and HER2 contributes to increased VEGF expression in granulosa cells of patients with OHSS†. In Biology of reproduction, 101, 426-432. doi:10.1093/biolre/ioz091. https://pubmed.ncbi.nlm.nih.gov/31167229/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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