C57BL/6JCya-Ezh1em1flox/Cya
Common Name:
Ezh1-flox
Product ID:
S-CKO-17805
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Ezh1-flox
Strain ID
CKOCMP-14055-Ezh1-B6J-VB
Gene Name
Product ID
S-CKO-17805
Gene Alias
ENX-2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ezh1em1flox/Cya mice (Catalog S-CKO-17805) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107285
NCBI RefSeq
NM_007970
Target Region
Exon 3~4
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Ezh1, the enhancer of zeste homolog 1, is an epigenetic silencing factor. As a core component of polycomb repressive complex 2 (PRC2), it mediates the mono-, di-and tri-methylation of histone H3 lysine 27 (H3K27me1/2/3), playing key roles in cell growth and differentiation [1,3]. It is involved in multiple biological processes such as embryonic hematopoiesis, oocyte meiosis, and neurogenesis [2,5,6].
In iPSC-derived T cell differentiation, Ezh1 repression facilitates in vitro differentiation and maturation of T cells, generating cells with enhanced antitumor activity [2]. In mouse oocytes and early embryos, maternal Ezh1/2 deficiency impairs mitochondrial structure and function, leading to oxidative stress, autophagy interference, and apoptosis [4]. In oocyte meiosis prophase I in mice, Ezh1/2 is essential for oocyte development, and its deficiency causes failure of DNA double-strand breaks repair and meiotic progression arrest [5]. In neurogenesis, both gain-and loss-of-function variants in Ezh1 disrupt neural progenitor cell differentiation, causing neurodevelopmental disorders [6].
In conclusion, Ezh1 is crucial for various biological processes including cell differentiation, oocyte and embryo development, and neurogenesis. Studies using gene knockout models in mice have revealed its role in these processes and associated disease conditions such as impaired T cell-based cancer immunotherapy, abnormal oocyte and embryo development, and neurodevelopmental disorders.
References:
1. An, Ran, Li, Yu-Qing, Lin, Yue-Ling, Li, Man-Mei, Liu, Zhong. 2022. EZH1/2 as targets for cancer therapy. In Cancer gene therapy, 30, 221-235. doi:10.1038/s41417-022-00555-1. https://pubmed.ncbi.nlm.nih.gov/36369341/
2. Jing, Ran, Scarfo, Irene, Najia, Mohamad Ali, Maus, Marcela V, Daley, George Q. . EZH1 repression generates mature iPSC-derived CAR T cells with enhanced antitumor activity. In Cell stem cell, 29, 1181-1196.e6. doi:10.1016/j.stem.2022.06.014. https://pubmed.ncbi.nlm.nih.gov/35931029/
3. Lee, Soo Hyun, Li, Yingying, Kim, Hanbyeol, Park, Kyumin, Lee, Chul-Hwan. . The role of EZH1 and EZH2 in development and cancer. In BMB reports, 55, 595-601. doi:. https://pubmed.ncbi.nlm.nih.gov/36476271/
4. Zhang, Dan, Deng, Wenbo, Jiang, Ting, Gao, Shaorong, Lu, Zhongxian. 2024. Maternal Ezh1/2 deficiency impairs the function of mitochondria in mouse oocytes and early embryos. In Journal of cellular physiology, 239, e31244. doi:10.1002/jcp.31244. https://pubmed.ncbi.nlm.nih.gov/38529784/
5. Jiang, Ting, Zhang, Chengxiu, Cao, Xinjing, Wang, Haibin, Lu, Zhongxian. 2024. EZH1/2 plays critical roles in oocyte meiosis prophase I in mice. In Biological research, 57, 83. doi:10.1186/s40659-024-00564-4. https://pubmed.ncbi.nlm.nih.gov/39511641/
6. Gracia-Diaz, Carolina, Zhou, Yijing, Yang, Qian, Martínez-Balbás, Marian A, Akizu, Naiara. 2023. Gain and loss of function variants in EZH1 disrupt neurogenesis and cause dominant and recessive neurodevelopmental disorders. In Nature communications, 14, 4109. doi:10.1038/s41467-023-39645-5. https://pubmed.ncbi.nlm.nih.gov/37433783/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen