C57BL/6JCya-Ifi27l2aem1flox/Cya
Common Name:
Ifi27l2a-flox
Product ID:
S-CKO-17818
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Ifi27l2a-flox
Strain ID
CKOCMP-76933-Ifi27l2a-B6J-VB
Gene Name
Product ID
S-CKO-17818
Gene Alias
2310061N23Rik; Ifi27; Isg12; Isg12(b1)
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ifi27l2aem1flox/Cya mice (Catalog S-CKO-17818) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000055071
NCBI RefSeq
NM_029803
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Ifi27l2a, also known as interferon alpha-inducible protein 27 like 2A, is an interferon-stimulated gene. It is induced by interferons for viral host defense and has been associated with pro-inflammatory cellular mechanisms [1,2,3,4,5]. It may be involved in multiple biological processes, with its role in neurodegeneration being of particular interest recently. Genetic models, such as gene knockout mice, have been crucial in exploring its functions.
In the context of aging and stroke, single-cell RNA sequencing showed Ifi27l2a was significantly up-regulated in microglia of aged mouse brains post-stroke [1,2]. Induction of Ifi27l2a in microglia can stimulate mitochondrial ROS production and promote a pro-inflammatory phenotype [1,2]. Hemizygous deletion of Ifi27l2a (Ifi27l2a+/- mice) reduced gliosis, acute and chronic brain injury, and motor function deficits in an ischemic stroke model, indicating it is a critical neuroinflammatory mediator in ischemic stroke [1,2]. In viral infection studies, Ifi27l2a -/- mice were more vulnerable to lethal West Nile virus (WNV) infection, with higher viral burden in the CNS, especially in the brain stem, cerebellum, and spinal cord. This suggests Ifi27l2a has an antiviral phenotype in subsets of cells [3]. However, deletion of Ifi27l2a in mice did not increase susceptibility to influenza A infection [4].
In conclusion, Ifi27l2a plays important roles in neuroinflammation, especially in the context of aging and stroke, as well as in antiviral defense in a cell-type-and region-specific manner. The use of Ifi27l2a KO mouse models has been instrumental in revealing its functions in these disease-related processes, providing potential therapeutic targets for ischemic stroke and insights into the body's antiviral mechanisms [1,2,3].
References:
1. Kim, Gab Seok, Harmon, Elisabeth, Gutierrez, Manuel C, Wythe, Joshua D, Marrelli, Sean P. 2025. Single-cell analysis identifies Ifi27l2a as a gene regulator of microglial inflammation in the context of aging and stroke in mice. In Nature communications, 16, 1639. doi:10.1038/s41467-025-56847-1. https://pubmed.ncbi.nlm.nih.gov/39953063/
2. Kim, Gab Seok, Harmon, Elisabeth, Gutierrez, Manuel, Wythe, Joshua, Marrelli, Sean. 2023. Single-cell analysis identifies Ifi27l2a as a novel gene regulator of microglial inflammation in the context of aging and stroke. In Research square, , . doi:10.21203/rs.3.rs-2557290/v1. https://pubmed.ncbi.nlm.nih.gov/36824976/
3. Lucas, Tiffany M, Richner, Justin M, Diamond, Michael S. 2015. The Interferon-Stimulated Gene Ifi27l2a Restricts West Nile Virus Infection and Pathogenesis in a Cell-Type- and Region-Specific Manner. In Journal of virology, 90, 2600-15. doi:10.1128/JVI.02463-15. https://pubmed.ncbi.nlm.nih.gov/26699642/
4. Tantawy, Mohamed A, Hatesuer, Bastian, Wilk, Esther, Weiß, Siegfried, Schughart, Klaus. 2014. The interferon-induced gene Ifi27l2a is active in lung macrophages and lymphocytes after influenza A infection but deletion of Ifi27l2a in mice does not increase susceptibility to infection. In PloS one, 9, e106392. doi:10.1371/journal.pone.0106392. https://pubmed.ncbi.nlm.nih.gov/25184786/
5. Al-Shujairi, Wisam H, Clarke, Jennifer N, Davies, Lorena T, Pitson, Stuart M, Carr, Jillian M. 2017. Intracranial Injection of Dengue Virus Induces Interferon Stimulated Genes and CD8+ T Cell Infiltration by Sphingosine Kinase 1 Independent Pathways. In PloS one, 12, e0169814. doi:10.1371/journal.pone.0169814. https://pubmed.ncbi.nlm.nih.gov/28095439/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen