C57BL/6JCya-Ubxn2aem1flox/Cya
Common Name:
Ubxn2a-flox
Product ID:
S-CKO-17831
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Ubxn2a-flox
Strain ID
CKOCMP-217379-Ubxn2a-B6J-VB
Gene Name
Product ID
S-CKO-17831
Gene Alias
6330407P03Rik; Ubxd4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ubxn2aem1flox/Cya mice (Catalog S-CKO-17831) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020962
NCBI RefSeq
NM_145441
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Ubxn2a, a ubiquitin-like protein, has been shown to play crucial roles in multiple biological processes. It is involved in regulating protein turnover within key signaling pathways, and is particularly important in cancer-related processes. Its functions are associated with pathways such as the mTORC2 signaling pathway, which is relevant to tumor progression, as well as processes related to the regulation of oncoproteins [1,2]. Genetic models, especially mouse models, have been valuable in studying its functions [1].
In murine models, haploinsufficiency of UBXN2A significantly increases colon tumorigenesis, indicating its role as a potential tumor suppressor [1]. UBXN2A targets Rictor, a key component of the mTORC2 complex, for 26S proteasomal degradation, thus suppressing the Rictor-mTORC2 signaling pathway [1]. This leads to reduced AKT phosphorylation downstream of the mTORC2 pathway, affecting cellular processes like cell migration [1]. Additionally, studies in mice have shown that UBXN2A enhances CHIP-mediated proteasomal degradation of the oncoprotein mortalin-2 (mot-2) in colon tissues [3].
In conclusion, Ubxn2a has essential functions in regulating protein degradation and signaling pathways. Mouse models, especially those with Ubxn2a haploinsufficiency, have revealed its significance in colon cancer development, highlighting its potential as a therapeutic target in colorectal cancer [1,3].
References:
1. Sane, Sanam, Srinivasan, Rekha, Potts, Rashaun A, Black, Adrian R, Rezvani, Khosrow. 2023. UBXN2A suppresses the Rictor-mTORC2 signaling pathway, an established tumorigenic pathway in human colorectal cancer. In Oncogene, 42, 1763-1776. doi:10.1038/s41388-023-02686-7. https://pubmed.ncbi.nlm.nih.gov/37037900/
2. Reihe, Casey. . UBXN2A, a Ubiquitin-Like Protein, Alters Proteins in mTORC2 Pathway. In South Dakota medicine : the journal of the South Dakota State Medical Association, 75, 456-458. doi:. https://pubmed.ncbi.nlm.nih.gov/36889268/
3. Sane, Sanam, Hafner, Andre, Srinivasan, Rekha, Yin, Jun, Rezvani, Khosrow. 2018. UBXN2A enhances CHIP-mediated proteasomal degradation of oncoprotein mortalin-2 in cancer cells. In Molecular oncology, 12, 1753-1777. doi:10.1002/1878-0261.12372. https://pubmed.ncbi.nlm.nih.gov/30107089/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen