C57BL/6JCya-Bpifb1em1flox/Cya
Common Name:
Bpifb1-flox
Product ID:
S-CKO-17834
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Bpifb1-flox
Strain ID
CKOCMP-228801-Bpifb1-B6J-VB
Gene Name
Product ID
S-CKO-17834
Gene Alias
Lplunc1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Bpifb1em1flox/Cya mice (Catalog S-CKO-17834) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000081816
NCBI RefSeq
NM_001012392
Target Region
Exon 3~6
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Bpifb1, also known as LPLUNC1, belongs to the BPI-fold-containing family. It is a natural immune protection molecule with bactericidal and osmotic enhancement protein domains, responding to external physical and chemical stimuli. It is involved in host defense, especially in airway mucociliary clearance (MCC), and may play roles in regulating chronic infections, inflammation, and tumor development [2].
In Bpifb1 knockout (KO) mice, effective MCC in vivo was diminished without defects in epithelial ion transport or ciliary beat frequency. Loss of Bpifb1 altered the biophysical and biochemical properties of mucus associated with impaired MCC, indicating its importance in the mucociliary apparatus and mucus protein network [1].
In nasopharyngeal carcinoma (NPC), Bpifb1 is downregulated. Overexpression of Bpifb1 promotes apoptosis, inhibits proliferation, migration, invasion, vasculogenic mimicry, and radioresistance in NPC cells through various mechanisms, such as regulating the MEK/ERK, JNK/AP1 signaling pathways, and interacting with proteins like VTN and VIM [3,5,6,7].
In hormone-receptor-positive breast cancer, high Bpifb1 expression is associated with lymph node metastasis, as it promotes metastasis by inducing macrophage M2-like polarization [4].
In cystic fibrosis (CF) lung disease, Bpifb1 is upregulated in human and mouse lungs [8].
In non-obese diabetic (NOD) mice, Bpifb1 mRNA is upregulated in parotid acinar cells, potentially predicting disease traits before autoimmunity onset [9]. Also, autoantibodies to Bpifb1 are associated with interstitial lung disease (ILD) in autoimmune polyglandular syndrome type 1 (APS1) and other ILD patients [10].
In conclusion, Bpifb1 plays crucial roles in multiple biological processes and diseases. The use of Bpifb1 KO mouse models has been instrumental in revealing its functions in airway MCC, tumor development in NPC and breast cancer, as well as its association with CF lung disease, potential disease prediction in NOD mice, and ILD. These findings enhance our understanding of Bpifb1's biological functions and its implications in disease mechanisms.
References：
1. Donoghue, Lauren J, Markovetz, Matthew R, Morrison, Cameron B, Hill, David B, Kelada, Samir N P. 2023. BPIFB1 loss alters airway mucus properties and diminishes mucociliary clearance. In American journal of physiology. Lung cellular and molecular physiology, 325, L765-L775. doi:10.1152/ajplung.00390.2022. https://pubmed.ncbi.nlm.nih.gov/37847709/
2. Li, Jie, Xu, Peng, Wang, Lingwei, Yu, Xiu, Lu, Yongzhen. . Molecular biology of BPIFB1 and its advances in disease. In Annals of translational medicine, 8, 651. doi:10.21037/atm-20-3462. https://pubmed.ncbi.nlm.nih.gov/32566588/
3. Jiang, Xianjie, Deng, Xiangying, Wang, Jie, Xiong, Wei, Zeng, Zhaoyang. 2021. BPIFB1 inhibits vasculogenic mimicry via downregulation of GLUT1-mediated H3K27 acetylation in nasopharyngeal carcinoma. In Oncogene, 41, 233-245. doi:10.1038/s41388-021-02079-8. https://pubmed.ncbi.nlm.nih.gov/34725462/
4. Hu, Anbang, Liu, Yansong, Zhang, Hanyu, Ma, Fei, Guo, Baoliang. 2023. BPIFB1 promotes metastasis of hormone receptor-positive breast cancer via inducing macrophage M2-like polarization. In Cancer science, 114, 4157-4171. doi:10.1111/cas.15957. https://pubmed.ncbi.nlm.nih.gov/37702269/
5. Wei, Fang, Tang, Le, He, Yi, Xiong, Wei, Zeng, Zhaoyang. 2018. BPIFB1 (LPLUNC1) inhibits radioresistance in nasopharyngeal carcinoma by inhibiting VTN expression. In Cell death & disease, 9, 432. doi:10.1038/s41419-018-0409-0. https://pubmed.ncbi.nlm.nih.gov/29568064/
6. Wei, Fang, Wu, Yingfen, Tang, Le, Xiong, Wei, Zeng, Zhaoyang. 2017. BPIFB1 (LPLUNC1) inhibits migration and invasion of nasopharyngeal carcinoma by interacting with VTN and VIM. In British journal of cancer, 118, 233-247. doi:10.1038/bjc.2017.385. https://pubmed.ncbi.nlm.nih.gov/29123267/
7. Xu, Yice, Tao, Zezhang, Jiang, Yang, Liu, Tao, Xiang, Yinzhou. 2019. Overexpression of BPIFB1 promotes apoptosis and inhibits proliferation via the MEK/ERK signal pathway in nasopharyngeal carcinoma. In International journal of clinical and experimental pathology, 12, 356-364. doi:. https://pubmed.ncbi.nlm.nih.gov/31933752/
8. Bingle, Lynne, Wilson, Kirsty, Musa, Maslinda, Mall, Marcus A, Bingle, Colin D. 2012. BPIFB1 (LPLUNC1) is upregulated in cystic fibrosis lung disease. In Histochemistry and cell biology, 138, 749-58. doi:10.1007/s00418-012-0990-8. https://pubmed.ncbi.nlm.nih.gov/22767025/
9. Nashida, T, Yoshimura, K, Yoshie, S, Mizuhashi, F, Shimomura-Kuroki, J. 2015. Upregulation of Bpifb1 expression in the parotid glands of non-obese diabetic mice. In Oral diseases, 22, 46-52. doi:10.1111/odi.12377. https://pubmed.ncbi.nlm.nih.gov/26769076/
10. Shum, Anthony K, Alimohammadi, Mohammad, Tan, Catherine L, Kämpe, Olle, Anderson, Mark S. . BPIFB1 is a lung-specific autoantigen associated with interstitial lung disease. In Science translational medicine, 5, 206ra139. doi:10.1126/scitranslmed.3006998. https://pubmed.ncbi.nlm.nih.gov/24107778/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen