C57BL/6JCya-Ogdhlem1flox/Cya
Common Name:
Ogdhl-flox
Product ID:
S-CKO-17846
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ogdhl-flox
Strain ID
CKOCMP-239017-Ogdhl-B6J-VB
Gene Name
Product ID
S-CKO-17846
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ogdhlem1flox/Cya mice (Catalog S-CKO-17846) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000228529
NCBI RefSeq
NM_001081130
Target Region
Exon 5~7
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Oxoglutarate dehydrogenase-like (OGDHL) is an isoenzyme of oxyglutarate dehydrogenase (OGDH) in the OGDH complex. It is involved in degrading glucose and glutamate, and plays a role in regulating glutamine metabolism, which is crucial for maintaining normal cellular metabolism and energy production [1,4]. It is also associated with important cellular pathways such as the tricarboxylic acid cycle [1].
In hepatocellular carcinoma (HCC), OGDHL knockdown promotes tumor cell proliferation, while overexpression inhibits growth in mouse xenograft models. OGDHL down-regulation leads to an up-regulation of the α-ketoglutarate (αKG):citrate ratio, driving reductive carboxylation of glutamine-derived αKG via retrograde tricarboxylic acid cycling and activating the mTORC1 signaling pathway in an αKG-dependent manner, thus promoting de novo lipogenesis [1]. In clear cell renal cell carcinoma (ccRCC), upregulation of OGDHL expression effectively inhibits ccRCC growth and metastasis both in vitro and in vivo [2]. In kidney renal clear cell carcinoma (KIRC), low OGDHL expression is associated with tumor progression and poor prognosis, and is related to differences in the proportion of tumor-infiltrating immune cells [3].
In conclusion, OGDHL is essential for normal cellular metabolism, especially in glutamine metabolism. Gene knockout or knockdown models in mice have revealed its tumor-suppressive role in multiple cancers such as HCC, ccRCC, and KIRC. Understanding the function of OGDHL through these models provides potential prognostic biomarkers and therapeutic targets for these cancer types [1,2,3].
References:
1. Dai, Weiqi, Xu, Ling, Yu, Xiangnan, Guo, Chuanyong, Shen, Xizhong. 2019. OGDHL silencing promotes hepatocellular carcinoma by reprogramming glutamine metabolism. In Journal of hepatology, 72, 909-923. doi:10.1016/j.jhep.2019.12.015. https://pubmed.ncbi.nlm.nih.gov/31899205/
2. Shi, Jian, Miao, Daojia, Lv, Qingyang, Xiong, Zhiyong, Zhang, Xiaoping. 2023. The m6A modification-mediated OGDHL exerts a tumor suppressor role in ccRCC by downregulating FASN to inhibit lipid synthesis and ERK signaling. In Cell death & disease, 14, 560. doi:10.1038/s41419-023-06090-7. https://pubmed.ncbi.nlm.nih.gov/37626050/
3. Xie, Zhouzhou, Chen, Nanhui. 2023. Low OGDHL expression affects the prognosis and immune infiltration of kidney renal clear cell carcinoma. In Translational cancer research, 12, 3045-3060. doi:10.21037/tcr-23-961. https://pubmed.ncbi.nlm.nih.gov/38130311/
4. Jiang, Xiang, Peng, Jin, Xie, Yuanyuan, Han, Junhai, Yu, Decai. 2023. Oxoglutarate dehydrogenase-like inhibits the progression of hepatocellular carcinoma by inducing DNA damage through non-canonical function. In Cell death and differentiation, 30, 1931-1942. doi:10.1038/s41418-023-01186-1. https://pubmed.ncbi.nlm.nih.gov/37419985/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen