C57BL/6JCya-Zfp36l1em1flox/Cya
Common Name:
Zfp36l1-flox
Product ID:
S-CKO-17848
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp36l1-flox
Strain ID
CKOCMP-12192-Zfp36l1-B6J-VA
Gene Name
Product ID
S-CKO-17848
Gene Alias
Berg36; Brf1; D530020L18Rik; ERF1; TIS11b; cMG1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp36l1em1flox/Cya mice (Catalog S-CKO-17848) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021552
NCBI RefSeq
NM_007564
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Zfp36l1, a member of the RNA-binding zinc finger protein 36 (ZFP36) family, contains tandem CCCH-type zinc-finger motifs. It is an mRNA-binding protein that destabilizes mRNAs containing adenylate-uridylate-rich elements (AREs) in their 3'-untranslated regions (3'UTR), playing a crucial role in post-transcriptional regulation of gene expression and participating in numerous physiological processes [1,2,3,4,5,6,7,8,9,10].
In various disease models, Zfp36l1 shows diverse functions. In small cell lung cancer (SCLC), LSD1 represses Zfp36l1, and upon LSD1 inhibition, Zfp36l1 expression restores, blocking SCLC neuroendocrine differentiation by destabilizing SOX2 and INSM1 mRNAs [1]. In osteosarcoma, low Zfp36l1 expression promotes lung metastasis by inhibiting the SDC4-TGF-β signaling feedback loop [4]. In alcoholic liver disease, liver-specific Zfp36l1-deficient mice are protected from alcohol-induced hepatic steatosis, injury, and inflammation, possibly by stabilizing Fgf21 mRNA [5]. In chronic myeloid leukemia cells, Nuclease technology-derived knockout of Zfp36l1 leads to decreased proliferation rates, and Zfp36l1 negatively regulates the cell cycle inhibitor CDKN1A [9]. In flavivirus infection, overexpression of Zfp36l1 reduces Japanese encephalitis virus and dengue virus infection by destabilizing the viral genome via 5'-3' XRN1 and 3'-5' RNA-exosome RNA decay pathways [10].
In conclusion, Zfp36l1 is a key regulator in post-transcriptional gene regulation. Through gene knockout and other in vivo models, it has been shown to play important roles in multiple disease areas, including cancer, liver disease, and viral infection. These findings provide insights into the molecular mechanisms of these diseases and potential therapeutic targets.
References:
1. Chen, Hsiao-Yun, Durmaz, Yavuz T, Li, Yixiang, Signoretti, Sabina, Oser, Matthew G. 2022. Regulation of neuroendocrine plasticity by the RNA-binding protein ZFP36L1. In Nature communications, 13, 4998. doi:10.1038/s41467-022-31998-7. https://pubmed.ncbi.nlm.nih.gov/36008402/
2. Wei, Xujin, Liu, Jie, Cheng, Jia, Cai, Jianchun, Zhuo, Huiqin. 2024. Super-enhancer-driven ZFP36L1 promotes PD-L1 expression in infiltrative gastric cancer. In eLife, 13, . doi:10.7554/eLife.96445. https://pubmed.ncbi.nlm.nih.gov/39373630/
3. Chen, Ningheng, Wu, Shiyong, Zhi, Kangkang, Zhang, Xiaoping, Guo, Xueli. 2024. ZFP36L1 controls KLF16 mRNA stability in vascular smooth muscle cells during restenosis after vascular injury. In Journal of molecular and cellular cardiology, 192, 13-25. doi:10.1016/j.yjmcc.2024.04.012. https://pubmed.ncbi.nlm.nih.gov/38653384/
4. Ma, Mengjun, Zhuang, Jiahao, Li, Hongyu, Wu, Yanfeng, Shen, Huiyong. 2023. Low expression of ZFP36L1 in osteosarcoma promotes lung metastasis by inhibiting the SDC4-TGF-β signaling feedback loop. In Oncogene, 43, 47-60. doi:10.1038/s41388-023-02880-7. https://pubmed.ncbi.nlm.nih.gov/37935976/
5. Bathula, Chandra S, Chen, Jian, Kumar, Rahul, Saini, Yogesh, Patial, Sonika. 2021. ZFP36L1 Regulates Fgf21 mRNA Turnover and Modulates Alcoholic Hepatic Steatosis and Inflammation in Mice. In The American journal of pathology, 192, 208-225. doi:10.1016/j.ajpath.2021.10.017. https://pubmed.ncbi.nlm.nih.gov/34774847/
6. Ding, Kang, Zhang, Fengping, Qi, Gaoxiu, Zheng, Jie, Zhou, Fenghua. . ZFP36L1 Promotes Gastric Cancer Progression via Regulating JNK and p38 MAPK Signaling Pathways. In Recent patents on anti-cancer drug discovery, 18, 80-91. doi:10.2174/1574892817666220524102403. https://pubmed.ncbi.nlm.nih.gov/35611776/
7. Rynne, Jennifer, Ortiz-Zapater, Elena, Bagley, Dustin C, Adcock, Ian M, Martinez-Nunez, Rocio T. 2023. The RNA binding proteins ZFP36L1 and ZFP36L2 are dysregulated in airway epithelium in human and a murine model of asthma. In Frontiers in cell and developmental biology, 11, 1241008. doi:10.3389/fcell.2023.1241008. https://pubmed.ncbi.nlm.nih.gov/37928904/
8. Loh, Xin-Yi, Sun, Qiao-Yang, Ding, Ling-Wen, Lin, De-Chen, Koeffler, H Phillip. 2019. RNA-Binding Protein ZFP36L1 Suppresses Hypoxia and Cell-Cycle Signaling. In Cancer research, 80, 219-233. doi:10.1158/0008-5472.CAN-18-2796. https://pubmed.ncbi.nlm.nih.gov/31551365/
9. Kaehler, Meike, Dworschak, Maike, Rodin, Julian Phillip, Cascorbi, Ingolf, Nagel, Inga. 2021. ZFP36L1 plays an ambiguous role in the regulation of cell expansion and negatively regulates CDKN1A in chronic myeloid leukemia cells. In Experimental hematology, 99, 54-64.e7. doi:10.1016/j.exphem.2021.05.006. https://pubmed.ncbi.nlm.nih.gov/34090970/
10. Chiu, Han, Chiu, Hsin-Ping, Yu, Han-Pang, Lin, Yi-Ling, Lin, Ren-Jye. 2021. Zinc Finger Protein ZFP36L1 Inhibits Flavivirus Infection by both 5'-3' XRN1 and 3'-5' RNA-Exosome RNA Decay Pathways. In Journal of virology, 96, e0166521. doi:10.1128/JVI.01665-21. https://pubmed.ncbi.nlm.nih.gov/34643435/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen