C57BL/6JCya-Ppp1cbem1flox/Cya
Common Name:
Ppp1cb-flox
Product ID:
S-CKO-17888
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ppp1cb-flox
Strain ID
CKOCMP-19046-Ppp1cb-B6J-VB
Gene Name
Product ID
S-CKO-17888
Gene Alias
1200010B19
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp1cbem1flox/Cya mice (Catalog S-CKO-17888) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000015100
NCBI RefSeq
NM_172707
Target Region
Exon 4~6
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Ppp1cb, also known as the beta-type catalytic subunit of serine/threonine-protein phosphatase 1, is involved in multiple biological processes. It participates in the RAS/MAPK pathway as it binds to the RAF1-PPP1CB complex [1]. It also plays a role in hepatic de novo lipogenesis, adipogenesis, and is involved in pathways related to insulin resistance, cancer glycolysis, and chemoresistance in bladder cancer [3,4,5,6,7].
In RASopathies, a de novo PPP1CB variant was identified in a patient with Noonan syndrome, and a recurrent p.Pro49Arg mutation in PPP1CB broadens the clinical phenotype of Noonan-like syndrome [1,2]. In adipogenesis, depletion of PPP1CB in 3T3-L1 preadipocytes suppresses their differentiation into mature adipocytes, suggesting it as a potential therapeutic target for obesity [6,7]. In bladder cancer, gemcitabine plus cisplatin therapy-induced NF-κB signaling promotes OIP5-mediated degradation of PPP1CB, leading to chemoresistance [5].
In conclusion, PPP1CB is crucial in various biological processes and disease conditions. Its role in RASopathies, adipogenesis, and cancer-related chemoresistance, as revealed through these functional studies, provides valuable insights into potential therapeutic strategies for these diseases.
References:
1. Umeki, Ikumi, Niihori, Tetsuya, Abe, Taiki, Kure, Shigeo, Aoki, Yoko. 2018. Delineation of LZTR1 mutation-positive patients with Noonan syndrome and identification of LZTR1 binding to RAF1-PPP1CB complexes. In Human genetics, 138, 21-35. doi:10.1007/s00439-018-1951-7. https://pubmed.ncbi.nlm.nih.gov/30368668/
2. Bertola, Débora, Yamamoto, Guilherme, Buscarilli, Michelle, Passos-Bueno, Maria Rita, Kim, Chong. . The recurrent PPP1CB mutation p.Pro49Arg in an additional Noonan-like syndrome individual: Broadening the clinical phenotype. In American journal of medical genetics. Part A, 173, 824-828. doi:10.1002/ajmg.a.38070. https://pubmed.ncbi.nlm.nih.gov/28211982/
3. Yao, Zhenyu, Gong, Ying, Chen, Wenbin, Bo, Tao, Zhao, Jiajun. 2023. Upregulation of WDR6 drives hepatic de novo lipogenesis in insulin resistance in mice. In Nature metabolism, 5, 1706-1725. doi:10.1038/s42255-023-00896-7. https://pubmed.ncbi.nlm.nih.gov/37735236/
4. Zhou, Yanling, Peng, Xintong, Fang, Cheng, Shi, Ying, Liu, Shuang. 2024. Histones Methyltransferase NSD3 Inhibits Lung Adenocarcinoma Glycolysis Through Interacting with PPP1CB to Decrease STAT3 Signaling Pathway. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2400381. doi:10.1002/advs.202400381. https://pubmed.ncbi.nlm.nih.gov/39119928/
5. Wang, Xianteng, Guo, Ting, Niu, Liman, Xu, Haibo, Huang, Weiren. 2024. Engineered targeting OIP5 sensitizes bladder cancer to chemotherapy resistance via TRIP12-PPP1CB-YBX1 axis. In Oncogene, 43, 2850-2867. doi:10.1038/s41388-024-03136-8. https://pubmed.ncbi.nlm.nih.gov/39155295/
6. Kim, Jinsoo, Ahn, Dohee, Chung, Sang J. 2022. Chebulinic Acid Suppresses Adipogenesis in 3T3-L1 Preadipocytes by Inhibiting PPP1CB Activity. In International journal of molecular sciences, 23, . doi:10.3390/ijms23020865. https://pubmed.ncbi.nlm.nih.gov/35055051/
7. Cho, Young-Lai, Min, Jeong-Ki, Roh, Kyung Min, Chung, Sang J, Kang, Hyo Jin. 2015. Phosphoprotein phosphatase 1CB (PPP1CB), a novel adipogenic activator, promotes 3T3-L1 adipogenesis. In Biochemical and biophysical research communications, 467, 211-7. doi:10.1016/j.bbrc.2015.10.004. https://pubmed.ncbi.nlm.nih.gov/26449462/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen