C57BL/6JCya-Plcd1em1flox/Cya
Common Name
Plcd1-flox
Product ID
S-CKO-17889
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-18799-Plcd1-B6J-VB
Status
When using this mouse strain in a publication, please cite “Plcd1-flox Mouse (Catalog S-CKO-17889) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Plcd1-flox
Strain ID
CKOCMP-18799-Plcd1-B6J-VB
Gene Name
Product ID
S-CKO-17889
Gene Alias
--
Background
C57BL/6JCya
Gene Full Name
phospholipase C, delta 1
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 9 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000214470
NCBI Transcript ID
NM_001293648
Target Region
Exon 3
Size of Effective Region
~1.5 kb
Overview of Gene Research
Plcd1, or Phospholipase C delta 1, encodes an enzyme involved in regulatory signaling of energy metabolism, calcium homeostasis, and intracellular movement [4]. It is essential for epidermal barrier integrity and plays a role in intracellular signal transduction [6].
Mutations in Plcd1 have been linked to various diseases. In families with multiple trichilemmal cysts, predisposing variants in PLCD1 were identified, suggesting a monoallelic mutational mechanism for cyst formation [1]. A germline missense variant of PLCD1 was found in a Chinese family with autosomal dominant multiple pilomatricomas. This variant increased the enzymatic activity of PLCδ1, leading to activation of the protein kinase C/protein kinase D/extracellular signal-regulated kinase1/2 pathway and TPRV6 channel closure, resulting in excessive keratinocyte proliferation and calcium accumulation in the tumors [6]. In addition, in chondrosarcoma, lower PLCD1 expression was detected mainly in high-grade tumors, and overexpressing PLCD1 induced DNA damage, causing cell cycle blocking and apoptosis [2]. In esophageal squamous cell carcinoma (ESCC), PLCD1 expression was downregulated, and upregulation of PLCD1 decreased the capacity of TE-1 and EC18 cells in proliferation, invasion, and migration, potentially through inhibition of the Wnt/β-catenin signaling pathway [3]. In breast cancer, PLCD1 was frequently downregulated/silenced by promoter methylation, and its ectopic expression inhibited colony formation, cell migration, and induced cell cycle G(2)/M arrest [4]. In colon cancer, miR-17-3p facilitated cancer cell progression by downregulating PLCD1 [5]. In oral squamous cell carcinoma, miR-191 regulated cell growth by targeting PLCD1 via the Wnt/β-catenin signaling pathway [7].
In conclusion, Plcd1 is involved in multiple biological processes and is associated with various diseases, including skin cysts, skin tumors, and several types of carcinomas. Studies on Plcd1, especially those using genetic models, have enhanced our understanding of its role in disease-related biological processes, potentially providing new therapeutic targets for these diseases.
References：
1. Shimomura, Yutaka, O'Shaughnessy, Ryan, Rajan, Neil. . PLCD1 and Pilar Cysts. In The Journal of investigative dermatology, 139, 2075-2077. doi:10.1016/j.jid.2019.05.027. https://pubmed.ncbi.nlm.nih.gov/31543210/
2. Shen, Jiakang, Yu, Chen, Wang, Zhuoying, Mu, Haoran, Cai, Zhengdong. 2022. PLCD1-Induced DNA Damage Inhibits the Tumor Growth via Downregulating CDKs in Chondrosarcoma. In Journal of oncology, 2022, 4488640. doi:10.1155/2022/4488640. https://pubmed.ncbi.nlm.nih.gov/35836489/
3. He, Xin, Meng, Fan, Yu, Zhong-Jian, Li, Ying, Zheng, Yan-Fang. 2020. PLCD1 Suppressed Cellular Proliferation, Invasion, and Migration via Inhibition of Wnt/β-Catenin Signaling Pathway in Esophageal Squamous Cell Carcinoma. In Digestive diseases and sciences, 66, 442-451. doi:10.1007/s10620-020-06218-1. https://pubmed.ncbi.nlm.nih.gov/32236884/
4. Xiang, Tingxiu, Li, Lili, Fan, Yichao, Tao, Qian, Ren, Guosheng. 2010. PLCD1 is a functional tumor suppressor inducing G(2)/M arrest and frequently methylated in breast cancer. In Cancer biology & therapy, 10, 520-7. doi:10.4161/cbt.10.5.12726. https://pubmed.ncbi.nlm.nih.gov/20657189/
5. Ji, Jinxing, Fu, Jun. 2022. MiR-17-3p Facilitates Aggressive Cell Phenotypes in Colon Cancer by Targeting PLCD1 Through Affecting KIF14. In Applied biochemistry and biotechnology, 195, 1723-1735. doi:10.1007/s12010-022-04218-7. https://pubmed.ncbi.nlm.nih.gov/36367621/
6. Liu, Kai, Luo, Junyu, Ma, Tingbin, Yi, Ping, Liu, Jing Yu. 2020. Germline Mutation of PLCD1 Contributes to Human Multiple Pilomatricomas through Protein Kinase D/Extracellular Signal-Regulated Kinase1/2 Cascade and TRPV6. In The Journal of investigative dermatology, 141, 533-544. doi:10.1016/j.jid.2020.05.121. https://pubmed.ncbi.nlm.nih.gov/32795530/
7. Wang, Zekun, Guan, Wenzhao, Ma, Yufeng, Wei, Jianing, Wang, Chenyang. 2023. MicroRNA-191 regulates oral squamous cell carcinoma cells growth by targeting PLCD1 via the Wnt/β-catenin signaling pathway. In BMC cancer, 23, 668. doi:10.1186/s12885-023-11113-9. https://pubmed.ncbi.nlm.nih.gov/37460940/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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