Logo
Homepage
Explore Our Models
My Cart
Contact
Subscribe
Models
Genetically Engineered Animals
Knockout Mice
Knockout Rats
Knockin Mice
Knockin Rats
Transgenic Mice
Transgenic Rats
Model Generation Techniques
Turboknockout<sup>®</sup> Gene Targeting
ES Cell Gene Targeting
Targeted Gene Editing
Regular Transgenic
PiggyBac Transgenesis
BAC Transgenic
Research Models
HUGO-GT™ Humanized Mice
Cre Mouse Lines
Humanized Target Gene Models
Metabolic Disease Models
Ophthalmic Disease Models
Neurological Disease Models
Autoimmune Disease Models
Immunodeficient Mouse Models
Humanized Immune System Mouse Models
Oncology & Immuno-oncology Models
Covid-19 Mouse Models
MouseAtlas Model Library
Knockout Cell Line Product Catalog
Tumor Cell Line Product Catalog
AAV Standard Product Catalog
Animal Supporting Services
Breeding Services
Cryopreservation & Recovery
Phenotyping Services
BAC Modification
Custom Cell Line Models
Induced Pluripotent Stem Cells (iPSCs)
Knockout Cell Lines
Knockin Cell Lines
Point Mutation Cell Lines
Overexpression Cell Lines
Virus Packaging
Adeno-associated Virus (AAV) Packaging
Lentivirus Packaging
Adenovirus Packaging
CRO Services
By Therapeutic Area
Oncology
Ophthalmology
Neuroscience
Metabolic & Cardiovascular Diseases
Autoimmune & Inflammatory
By Drug Type
AI-Powered AAV Discovery
Gene Therapy
Oligonucleotide Therapy
Antibody Therapy
Cell Immunotherapy
Resources
Promotion
Events & Webinars
Newsroom
Blogs & Insights
Resource Vault
Reference Databases
Peer-Reviewed Citations
Rare Disease Data Center
AbSeek
Cell iGeneEditor™ System
OriCell
Quality
Facility Overview
Animal Health & Welfare
Health Reports
About Us
Corporate Overview
Our Partners
Careers
Contact Us
Login
Request a Product Quote
Select products from our catalogs and submit your request. Our team will get back to you with detailed information.
Full Name
Email
Phone Number
Organization
Job Role
Country
Catalog Type
Product Name
Additional Comments
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.
C57BL/6NCya-Ythdf2em1flox/Cya
Common Name:
Ythdf2-flox
Product ID:
S-CKO-17904
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ythdf2-flox
Strain ID
CKOCMP-213541-Ythdf2-B6N-VA
Gene Name
Ythdf2
Product ID
S-CKO-17904
Gene Alias
9430020E02Rik; HGRG8; NY-REN-2
Background
C57BL/6NCya
NCBI ID
213541
Modification
Conditional knockout
Chromosome
4
Phenotype
MGI:2444233
Document
Click here to download >>
Application
--
More
Rare Disease Data Center >>
Note
Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Ythdf2em1flox/Cya mice (Catalog S-CKO-17904) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000152796
NCBI RefSeq
NM_145393
Target Region
Exon 3
Size of Effective Region
~1.0 kb
Detailed Document
Click here to download >>
Overview of Gene Research
Ythdf2, the YT521-B homology domain 2, is a crucial N6-methyladenosine (m6A) reader protein. It mainly mediates the degradation of m6A-modified mRNAs, thus regulating gene expression at the post-transcriptional level. This function is involved in multiple biological pathways, such as NF-κB signaling, and is of great significance in various biological processes and disease conditions. Genetic models, especially knockout (KO) and conditional knockout (CKO) mouse models, have been instrumental in studying its functions.

In myeloid cells, loss of Ythdf2 in murine models augments antitumor immunity and overcomes tumor radioresistance by altering myeloid-derived suppressor cell (MDSC) differentiation and inhibiting MDSC infiltration and suppressive function. Ionizing radiation (IR)-induced YTHDF2 expression relies on NF-κB signaling, and YTHDF2 in turn activates NF-κB by degrading transcripts encoding negative regulators of NF-κB signaling, forming an IR-YTHDF2-NF-κB circuit [1]. In tumor-associated macrophages (TAMs), YTHDF2 deficiency suppresses tumor growth by reprogramming TAMs toward an antitumoral phenotype and enhancing CD8+ T cell-mediated antitumor immunity, with its expression in TAMs regulated by interleukin-10-STAT3 signaling [2]. In MYC-driven triple-negative breast cancer (TNBC) cells, disrupting YTHDF2-dependent mRNA degradation triggers apoptosis, as YTHDF2 interacts with mRNAs encoding proteins in the MAPK pathway, and its depletion leads to endoplasmic reticulum stress-induced apoptosis [3].

In conclusion, Ythdf2 plays a vital role in regulating gene expression through mRNA degradation. Studies using KO/CKO mouse models have revealed its significance in cancer-related processes, such as tumor radioresistance, TAM-mediated antitumor immunity, and cancer cell apoptosis. These findings suggest that Ythdf2 could be a potential therapeutic target in cancer treatment.

References:
1. Wang, Liangliang, Dou, Xiaoyang, Chen, Shijie, He, Chuan, Weichselbaum, Ralph R. 2023. YTHDF2 inhibition potentiates radiotherapy antitumor efficacy. In Cancer cell, 41, 1294-1308.e8. doi:10.1016/j.ccell.2023.04.019. https://pubmed.ncbi.nlm.nih.gov/37236197/
2. Ma, Shoubao, Sun, Baofa, Duan, Songqi, Caligiuri, Michael A, Yu, Jianhua. 2023. YTHDF2 orchestrates tumor-associated macrophage reprogramming and controls antitumor immunity through CD8+ T cells. In Nature immunology, 24, 255-266. doi:10.1038/s41590-022-01398-6. https://pubmed.ncbi.nlm.nih.gov/36658237/
3. Einstein, Jaclyn M, Perelis, Mark, Chaim, Isaac A, Westbrook, Thomas F, Yeo, Gene W. 2021. Inhibition of YTHDF2 triggers proteotoxic cell death in MYC-driven breast cancer. In Molecular cell, 81, 3048-3064.e9. doi:10.1016/j.molcel.2021.06.014. https://pubmed.ncbi.nlm.nih.gov/34216543/
Quality Control Standard
Sperm Test

Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.

Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.

Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Model Library
Model Library
Resources
Resources
Animal Quality
Animal Quality
Get Support
Get Support
Address:
2255 Martin Avenue, Suite E Santa Clara, CA 95050-2709, US
Tel:
800-921-8930 (8-6pm PST)
+1408-963-0306 (lnt’l)
Fax:
408-969-0338
Email:
animal-service@cyagen.com
service@cyagen.us
CRO Services
OncologyOphthalmologyNeuroscienceMetabolic & CardiovascularAutoimmune & InflammatoryGene TherapyAntibody Therapy
About Us
Corporate OverviewOur PartnersCareersContact Us
Social Media
Disclaimer: Pricing and availability of our products and services vary by region. Listed prices are applicable to the specific countries. Please contact us for more information.
Copyright © 2025 Cyagen. All rights reserved.
Privacy Policy
Site Map
Stay Updated with the Latest from Cyagen
Get the latest news on our research models, CRO services, scientific resources, and special offers—tailored to your research needs and delivered straight to your inbox.
Full Name
Email
Organization
Country
Areas of Interest