C57BL/6JCya-Mbd3l2em1flox/Cya
Common Name:
Mbd3l2-flox
Product ID:
S-CKO-17922
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mbd3l2-flox
Strain ID
CKOCMP-234988-Mbd3l2-B6J-VB
Gene Name
Product ID
S-CKO-17922
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mbd3l2em1flox/Cya mice (Catalog S-CKO-17922) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000213777
NCBI RefSeq
NM_144934.3
Target Region
Exon 3
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Mbd3l2, methyl-CpG-binding domain protein 3-like 2, is a protein-coding gene. It lacks the methyl-CpG-binding domain but is homologous to MBD2 and MBD3. It is involved in epigenetic regulation pathways, potentially influencing transcriptional modulation [2,4]. Mbd3l2 may play a role in processes related to DNA methylation and demethylation, which are crucial for normal development and disease-related gene regulation [1].
Gene knockout studies in mice showed that Mbd3l2 knockout mice were viable and fertile, suggesting that Mbd3l2 is dispensable for early development, fertility, and zygotic DNA demethylation in mice [3]. In another aspect, Mbd3l2 can promote Tet2 enzymatic activity, facilitating the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC), with potential implications in cancer development as Tet2 mutations are common in human malignancies [1]. Also, Mbd3l2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing, acting as a transcriptional modulator [2].
In summary, Mbd3l2 appears to have functions in epigenetic regulation, especially in relation to DNA methylation-associated processes. Although Mbd3l2 knockout mice show normal early development and fertility, its role in promoting Tet2 activity and modulating transcriptional repression provides insights into cancer-related epigenetic dysregulation, highlighting its importance in understanding disease mechanisms [1,2,3].
References:
1. Peng, Lina, Li, Yan, Xi, Yanping, Wu, Feizhen, Yu, Wenqiang. 2016. MBD3L2 promotes Tet2 enzymatic activity for mediating 5-methylcytosine oxidation. In Journal of cell science, 129, 1059-71. doi:10.1242/jcs.179044. https://pubmed.ncbi.nlm.nih.gov/26769901/
2. Jin, Seung-Gi, Jiang, Chun-Ling, Rauch, Tibor, Li, Hongwei, Pfeifer, Gerd P. 2005. MBD3L2 interacts with MBD3 and components of the NuRD complex and can oppose MBD2-MeCP1-mediated methylation silencing. In The Journal of biological chemistry, 280, 12700-9. doi:. https://pubmed.ncbi.nlm.nih.gov/15701600/
3. Jin, Seung-Gi, Tsark, Walter, Szabó, Piroska E, Pfeifer, Gerd P. . Haploid male germ cell- and oocyte-specific Mbd3l1 and Mbd3l2 genes are dispensable for early development, fertility, and zygotic DNA demethylation in the mouse. In Developmental dynamics : an official publication of the American Association of Anatomists, 237, 3435-43. doi:10.1002/dvdy.21767. https://pubmed.ncbi.nlm.nih.gov/18942147/
4. Jiang, Chun Ling, Jin, Seung Gi, Lee, Dong Hyun, Cooney, Austin J, Pfeifer, Gerd P. . MBD3L1 and MBD3L2, two new proteins homologous to the methyl-CpG-binding proteins MBD2 and MBD3: characterization of MBD3L1 as a testis-specific transcriptional repressor. In Genomics, 80, 621-9. doi:. https://pubmed.ncbi.nlm.nih.gov/12504854/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen