C57BL/6JCya-Dcp1aem1flox/Cya
Common Name:
Dcp1a-flox
Product ID:
S-CKO-17977
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dcp1a-flox
Strain ID
CKOCMP-75901-Dcp1a-B6J-VB
Gene Name
Product ID
S-CKO-17977
Gene Alias
1110066A22Rik; 4930568L04Rik; D14Ertd817e; Mitc1; SMIF
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
14
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dcp1aem1flox/Cya mice (Catalog S-CKO-17977) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000022535
NCBI RefSeq
NM_133761
Target Region
Exon 4~5
Size of Effective Region
~3.3 kb
Detailed Document
Overview of Gene Research
Dcp1a, the mRNA decapping factor, is a key player in post-transcriptional regulation of gene expression in eukaryotes. It is involved in mRNA cap removal before degradation and is associated with P-bodies. Its function impacts various biological processes, making it biologically significant. Genetic models, such as mouse models, have been valuable in studying its functions in vivo [1].
In mice, Dcp1a-deficient embryos generated using the Nuclease technology system showed embryonic lethality around E10.5, along with growth retardation and cardiac developmental defects, indicating its essential role in embryonic growth [1]. In mouse embryonic stem cells (ESCs), DCP1A, phosphorylated by MEK, regulates ESC self-renewal and differentiation through modulating P-body formation [2]. In the context of porcine deltacoronavirus (PDCoV) infection, PDCoV nsp5 cleaves DCP1A, reducing its antiviral activity, and this cleavage is a common immune evasion mechanism among different coronaviruses [3]. In gastric cancer, high DCP1a expression is associated with cancer development, poor prognosis, and chemotherapy resistance, and down-regulating its expression can reduce these effects [4].
In conclusion, Dcp1a is essential for embryonic growth, regulates the self-renewal and differentiation of embryonic stem cells, and is involved in viral immune evasion and cancer-related processes. Studies using gene-knockout mouse models have significantly contributed to understanding its role in these biological and disease-related contexts, providing insights into potential therapeutic targets for conditions like hepatocellular carcinoma and gastric cancer [1,2,3,4].
References:
1. Ibayashi, Megumi, Aizawa, Ryutaro, Tsukamoto, Satoshi. 2021. mRNA decapping factor Dcp1a is essential for embryonic growth in mice. In Biochemical and biophysical research communications, 555, 128-133. doi:10.1016/j.bbrc.2021.03.117. https://pubmed.ncbi.nlm.nih.gov/33813271/
2. Yu, Jiayu, Zhao, Nannan, Wang, Yuying, Zhang, Kai, Chen, Lingyi. 2024. DCP1A, a MEK substrate, regulates the self-renewal and differentiation of mouse embryonic stem cells. In Cell reports, 43, 115058. doi:10.1016/j.celrep.2024.115058. https://pubmed.ncbi.nlm.nih.gov/39671288/
3. Zhu, Xinyu, Chen, Jiyao, Tian, Liyuan, Fang, Liurong, Xiao, Shaobo. 2020. Porcine Deltacoronavirus nsp5 Cleaves DCP1A To Decrease Its Antiviral Activity. In Journal of virology, 94, . doi:10.1128/JVI.02162-19. https://pubmed.ncbi.nlm.nih.gov/32461317/
4. Ruan, Tuo, Zhang, Yazhi, Liu, Weizhen, Tao, Kaixiong, Wu, Chuanqing. 2020. Expression of DCP1a in gastric cancer and its biological function and mechanism in chemotherapy resistance in gastric cancer cells. In Digestive and liver disease : official journal of the Italian Society of Gastroenterology and the Italian Association for the Study of the Liver, 52, 1351-1358. doi:10.1016/j.dld.2020.06.031. https://pubmed.ncbi.nlm.nih.gov/32646734/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen