C57BL/6JCya-Upp1em1flox/Cya
Common Name:
Upp1-flox
Product ID:
S-CKO-17980
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Upp1-flox
Strain ID
CKOCMP-22271-Upp1-B6J-VB
Gene Name
Product ID
S-CKO-17980
Gene Alias
UPase; UdRPase; Up; Upp
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Upp1em1flox/Cya mice (Catalog S-CKO-17980) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000020677
NCBI RefSeq
NM_009477
Target Region
Exon 6
Size of Effective Region
~0.6 kb
Detailed Document
Overview of Gene Research
UPP1, also known as uridine phosphorylase 1, is a dimeric enzyme that plays a crucial role in pyrimidine salvage and uridine homeostasis [2]. It catalyzes the reversible phosphorylation of uridine to uracil and ribose-1-phosphate, and its activity is involved in multiple metabolic pathways, including those related to energy production and nucleotide synthesis [3].
In various cancers, UPP1 has been shown to promote tumor progression. In lung adenocarcinoma (LUAD), UPP1-high tumor cells are associated with an immunosuppressive microenvironment. UPP1 upregulation leads to increased release of immunosuppressive cytokines like TGF-β1 and elevates PD-L1 expression via the PI3K/AKT/mTOR pathway, suppressing CD8+ T cells [1]. Additionally, UPP1 drives glycolytic metabolism in LUAD, and its expression is subject to epigenetic regulation through histone acetylation [2]. In bladder cancer, UPP1 promotes cell proliferation, migration, invasion, and gemcitabine resistance by activating the AKT signaling pathway [3]. In gliomas, high levels of UPP1 are linked to poor survival, and it promotes tumor cell proliferation, invasion, and immune evasion [5]. In pancreatic ductal adenocarcinoma, UPP1 liberates uridine-derived ribose to fuel central carbon metabolism in glucose-restricted conditions [4].
In conclusion, UPP1 is a key enzyme in pyrimidine metabolism with significant implications in cancer progression. Studies, although not specifically highlighting KO/CKO mouse models in the provided references, suggest its role in promoting tumorigenesis and immunosuppression in multiple cancer types, making it a potential therapeutic target for these diseases.
References:
1. Li, Yin, Jiang, Manling, Aye, Ling, Ge, Di, Lu, Chunlai. 2024. UPP1 promotes lung adenocarcinoma progression through the induction of an immunosuppressive microenvironment. In Nature communications, 15, 1200. doi:10.1038/s41467-024-45340-w. https://pubmed.ncbi.nlm.nih.gov/38331898/
2. Wang, Xuan, Wang, Zheng, Huang, Renhong, Chen, Xiaofeng, Huang, Dayu. 2022. UPP1 Promotes Lung Adenocarcinoma Progression through Epigenetic Regulation of Glycolysis. In Aging and disease, 13, 1488-1503. doi:10.14336/AD.2022.0218. https://pubmed.ncbi.nlm.nih.gov/36186123/
3. Du, Wenzhi, Tu, Sheng, Zhang, Wenxiu, Wang, Xinghuan, Ju, Lingao. 2024. UPP1 enhances bladder cancer progression and gemcitabine resistance through AKT. In International journal of biological sciences, 20, 1389-1409. doi:10.7150/ijbs.83774. https://pubmed.ncbi.nlm.nih.gov/38385072/
4. Nwosu, Zeribe C, Ward, Matthew H, Sajjakulnukit, Peter, Sadanandam, Anguraj, Lyssiotis, Costas A. 2023. Uridine-derived ribose fuels glucose-restricted pancreatic cancer. In Nature, 618, 151-158. doi:10.1038/s41586-023-06073-w. https://pubmed.ncbi.nlm.nih.gov/37198494/
5. Chen, Zigui, Liu, Chao, Zhang, Chunyuan, Miao, Changfeng, Luo, Qisheng. 2024. Machine learning-based discovery of UPP1 as a key oncogene in tumorigenesis and immune escape in gliomas. In Frontiers in immunology, 15, 1475206. doi:10.3389/fimmu.2024.1475206. https://pubmed.ncbi.nlm.nih.gov/39380997/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen