C57BL/6JCya-Malat1em1flox/Cya
Common Name:
Malat1-flox
Product ID:
S-CKO-17987
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Malat1-flox
Strain ID
CKOCMP-72289-Malat1-B6J-VA
Gene Name
Product ID
S-CKO-17987
Gene Alias
2210401K01Rik; 9430072K23Rik; Neat2
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Malat1em1flox/Cya mice (Catalog S-CKO-17987) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000172812
NCBI RefSeq
NR_002847
Target Region
Exon 1
Size of Effective Region
~19.6 kb
Detailed Document
Overview of Gene Research
Malat1, short for Metastasis-associated lung adenocarcinoma transcript-1, is a highly conserved long non-coding RNA (lncRNA) [1,2,3,4,5,6,7,8,9,10]. It is retained in the nucleus and is abundantly expressed in cells and tissues. Malat1 regulates genes at transcriptional and post-transcriptional levels and is involved in multiple cancer-related pathways such as MAPK/ERK, PI3K/AKT, β-catenin/Wnt, Hippo, VEGF, YAP etc [2]. It has been shown to play a significant role in the development and progression of numerous diseases, especially cancer [1,2,3,4,5,6,7,8,9,10]. Genetic models, like gene knockout (KO) and conditional knockout (CKO) mouse models, are valuable for studying Malat1's functions in vivo.
In a mouse model of myocardial infarction, targeted knockdown of Malat1 in endothelial cells exacerbated oxidative stress, attenuated angiogenesis and microvascular perfusion, and decreased cardiac function. Further studies indicated that Malat1 acts as a competing endogenous RNA for miR-26b-5p and forms a signalling axis with Mfn1 to regulate mitochondrial dynamics and endothelial functions [5]. In chondrocytes, MALAT1 knockdown inhibited cell proliferation, promoted apoptosis, and increased extracellular matrix degradation via the miR-150-5p/AKT3 axis [6].
In conclusion, Malat1 is a crucial lncRNA involved in regulating gene expression and multiple biological processes. Studies using KO/CKO mouse models have revealed its role in diseases such as cardiovascular diseases and osteoarthritis. Understanding Malat1's functions through these models provides insights into disease mechanisms and potential therapeutic targets.
References:
1. Xu, Wen-Wen, Jin, Jin, Wu, Xiao-Yu, Ren, Qing-Ling, Farzaneh, Maryam. 2022. MALAT1-related signaling pathways in colorectal cancer. In Cancer cell international, 22, 126. doi:10.1186/s12935-022-02540-y. https://pubmed.ncbi.nlm.nih.gov/35305641/
2. Goyal, Bela, Yadav, Shashi Ranjan Mani, Awasthee, Nikee, Kunnumakkara, Ajaikumar B, Gupta, Subash Chandra. 2021. Diagnostic, prognostic, and therapeutic significance of long non-coding RNA MALAT1 in cancer. In Biochimica et biophysica acta. Reviews on cancer, 1875, 188502. doi:10.1016/j.bbcan.2021.188502. https://pubmed.ncbi.nlm.nih.gov/33428963/
3. Arun, Gayatri, Aggarwal, Disha, Spector, David L. 2020. MALAT1 Long Non-Coding RNA: Functional Implications. In Non-coding RNA, 6, . doi:10.3390/ncrna6020022. https://pubmed.ncbi.nlm.nih.gov/32503170/
4. Tufail, Muhammad. 2023. The MALAT1-breast cancer interplay: insights and implications. In Expert review of molecular diagnostics, 23, 665-678. doi:10.1080/14737159.2023.2233902. https://pubmed.ncbi.nlm.nih.gov/37405385/
5. Chen, Yuqiong, Li, Su, Zhang, Yan, Lu, Yao, Jia, Dalin. 2021. The lncRNA Malat1 regulates microvascular function after myocardial infarction in mice via miR-26b-5p/Mfn1 axis-mediated mitochondrial dynamics. In Redox biology, 41, 101910. doi:10.1016/j.redox.2021.101910. https://pubmed.ncbi.nlm.nih.gov/33667993/
6. Zhang, Ying, Wang, Fuyou, Chen, Guangxing, He, Rui, Yang, Liu. 2019. LncRNA MALAT1 promotes osteoarthritis by modulating miR-150-5p/AKT3 axis. In Cell & bioscience, 9, 54. doi:10.1186/s13578-019-0302-2. https://pubmed.ncbi.nlm.nih.gov/31304004/
7. Wu, Wenzheng, Wang, Shihao, Zhang, Lu, Mou, Yunying, Yan, Peizheng. 2022. Mechanistic studies of MALAT1 in respiratory diseases. In Frontiers in molecular biosciences, 9, 1031861. doi:10.3389/fmolb.2022.1031861. https://pubmed.ncbi.nlm.nih.gov/36419933/
8. Li, Zhi-Xing, Zhu, Qiong-Ni, Zhang, Hai-Bo, Wang, Guo, Zhu, Yuan-Shan. 2018. MALAT1: a potential biomarker in cancer. In Cancer management and research, 10, 6757-6768. doi:10.2147/CMAR.S169406. https://pubmed.ncbi.nlm.nih.gov/30584369/
9. Qiao, Feng-Hua, Tu, Min, Liu, Hong-Yan. 2021. Role of MALAT1 in gynecological cancers: Pathologic and therapeutic aspects. In Oncology letters, 21, 333. doi:10.3892/ol.2021.12594. https://pubmed.ncbi.nlm.nih.gov/33692865/
10. Zhao, Miaomiao, Wang, Songpo, Li, Qi, Guo, Piaoting, Liu, Xiaowei. 2018. MALAT1: A long non-coding RNA highly associated with human cancers. In Oncology letters, 16, 19-26. doi:10.3892/ol.2018.8613. https://pubmed.ncbi.nlm.nih.gov/29928382/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen