C57BL/6JCya-Saa2em1flox/Cya
Common Name:
Saa2-flox
Product ID:
S-CKO-17995
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Saa2-flox
Strain ID
CKOCMP-20209-Saa2-B6J-VB
Gene Name
Product ID
S-CKO-17995
Gene Alias
Saa-2; Saa1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Saa2em1flox/Cya mice (Catalog S-CKO-17995) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000210769
NCBI RefSeq
NM_011314
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Saa2 is one of the members of the Serum Amyloid A (SAA) protein family. SAA proteins are small, well-conserved in mammalian evolution, and are prominently synthesized in the liver. Saa2, along with SAA1, are key players in the acute phase response. They are lipophilic and contribute to high-density lipoproteins and cholesterol transport. Saa2 is also involved in various biological processes such as tissue remodeling, immune regulation, and has potential implications in host defense [1].
In a mouse model of intrauterine inflammation, placental Saa2 increased significantly. Maternal inhibition of Saa2 using siSaa2 markedly decreased preterm birth and downregulated the increased placental expression of pro-inflammatory cytokines Il1β, Il6, and Tnfα [3]. In the context of Th17-mediated inflammatory diseases, loss-and gain-of-function mouse models showed that Saa2, along with SAA1 and SAA3, have distinct systemic and local functions in promoting such diseases [2]. In inflammatory bowel disease, the expression of mesentery-derived Saa2 is upregulated, contributing to macrophage immunoregulation [4].
In conclusion, Saa2 is involved in multiple biological processes, especially in inflammation-related responses. Gene-knockout or loss-of-function mouse models have revealed its role in preterm birth, Th17-mediated inflammatory diseases, and inflammatory bowel disease, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Sack, George H. . Serum Amyloid A (SAA) Proteins. In Sub-cellular biochemistry, 94, 421-436. doi:10.1007/978-3-030-41769-7_17. https://pubmed.ncbi.nlm.nih.gov/32189310/
2. Lee, June-Yong, Hall, Jason A, Kroehling, Lina, Khanna, Kamal M, Littman, Dan R. 2019. Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease. In Cell, 180, 79-91.e16. doi:10.1016/j.cell.2019.11.026. https://pubmed.ncbi.nlm.nih.gov/31866067/
3. Liu, Yang, Liu, Jin, Liu, Anguo, Burd, Irina, Lei, Jun. 2022. Maternal siRNA silencing of placental SAA2 mitigates preterm birth following intrauterine inflammation. In Frontiers in immunology, 13, 902096. doi:10.3389/fimmu.2022.902096. https://pubmed.ncbi.nlm.nih.gov/36211368/
4. Lu, Huiying, Suo, Zhimin, Lin, Jian, Cong, Yingzi, Liu, Zhanju. 2024. Monocyte-macrophages modulate intestinal homeostasis in inflammatory bowel disease. In Biomarker research, 12, 76. doi:10.1186/s40364-024-00612-x. https://pubmed.ncbi.nlm.nih.gov/39095853/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen