C57BL/6JCya-Mepeem1flox/Cya
Common Name:
Mepe-flox
Product ID:
S-CKO-18005
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mepe-flox
Strain ID
CKOCMP-94111-Mepe-B6J-VB
Gene Name
Product ID
S-CKO-18005
Gene Alias
Of45
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mepeem1flox/Cya mice (Catalog S-CKO-18005) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000066207
NCBI RefSeq
NM_053172
Target Region
Exon 3
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Mepe, short for matrix extracellular phosphoglycoprotein, belongs to the SIBLING protein family. It plays a crucial role in regulating systemic phosphate homeostasis and mineralization, being involved in a hormone/enzyme/extracellular matrix protein cascade with FGF23 and PHEX [1,3,5]. This gene is also important in craniofacial and dental matrix maturation [2].
Analysis of Mepe null mouse molars showed that while the overall mineralized tooth volume and density of enamel and dentin were comparable to wild-type samples, there were increased thicknesses of predentin, dentin, and enamel, along with decreased gene expression of Enam, Bsp, Dmp1, Dspp, and Opn [2]. Additionally, a loss-of-function (LoF) mutation in MEPE was associated with decreased ultradistal forearm bone mineral density (BMD), increased osteoporosis, and fracture risk, which was further evaluated in UK and Icelandic samples [4].
In conclusion, Mepe is essential for matrix maturation in craniofacial and dental tissues and for maintaining normal bone mineral density. The study of Mepe null mouse models has revealed its role in these processes, highlighting its significance in understanding diseases related to abnormal mineralization and bone density, such as osteoporosis and certain dental disorders.
References：
1. Quarles, L Darryl. . FGF23, PHEX, and MEPE regulation of phosphate homeostasis and skeletal mineralization. In American journal of physiology. Endocrinology and metabolism, 285, E1-9. doi:. https://pubmed.ncbi.nlm.nih.gov/12791601/
2. Gullard, Angela, Gluhak-Heinrich, Jelica, Papagerakis, Silvana, Chen, Shuo, MacDougall, Mary. 2016. MEPE Localization in the Craniofacial Complex and Function in Tooth Dentin Formation. In The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 64, 224-36. doi:10.1369/0022155416635569. https://pubmed.ncbi.nlm.nih.gov/26927967/
3. Rowe, Peter S N. 2004. The wrickkened pathways of FGF23, MEPE and PHEX. In Critical reviews in oral biology and medicine : an official publication of the American Association of Oral Biologists, 15, 264-81. doi:. https://pubmed.ncbi.nlm.nih.gov/15470265/
4. Surakka, Ida, Fritsche, Lars G, Zhou, Wei, Hveem, Kristian, Willer, Cristen J. 2020. MEPE loss-of-function variant associates with decreased bone mineral density and increased fracture risk. In Nature communications, 11, 4093. doi:10.1038/s41467-020-17315-0. https://pubmed.ncbi.nlm.nih.gov/33097703/
5. Rowe, Peter S N. . Regulation of bone-renal mineral and energy metabolism: the PHEX, FGF23, DMP1, MEPE ASARM pathway. In Critical reviews in eukaryotic gene expression, 22, 61-86. doi:. https://pubmed.ncbi.nlm.nih.gov/22339660/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen