C57BL/6JCya-Crtac1em1flox/Cya
Common Name:
Crtac1-flox
Product ID:
S-CKO-18011
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Crtac1-flox
Strain ID
CKOCMP-72832-Crtac1-B6J-VB
Gene Name
Product ID
S-CKO-18011
Gene Alias
2810454P21Rik; Crtac1B; Lotus
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Crtac1em1flox/Cya mice (Catalog S-CKO-18011) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000238290
NCBI RefSeq
NM_001356589
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Crtac1, also known as cartilage acidic protein 1, is a glycosylated calcium-binding extracellular matrix protein. It has roles in the development, function, and repair of the nervous system. Structurally, it consists of a three-domain fold with a compact β-propeller-TTR combination, stabilized by potassium ions [6].
In non-small cell lung cancer (NSCLC), overexpression of Crtac1 increases the chemosensitivity to cisplatin. It promotes NFAT transcriptional activation by increasing intracellular Ca2+ levels, inducing STUB1 expression, accelerating Akt1 protein degradation, and enhancing cisplatin-induced apoptosis [1]. In urothelial carcinoma, low Crtac1 expression is associated with aggressive characteristics and poor prognosis, while exogenous expression suppresses cell proliferation, invasion, and angiogenesis [5]. In bladder cancer, Crtac1 inhibits cell proliferation, migration, invasion, and epithelial-mesenchymal transition by downregulating Yin Yang 1 to inactivate the TGF-β pathway [7]. In spinal ligament degeneration, CRTAC1+ chondrocyte-like cells are involved in the pathogenesis, interacting with SPP1+ macrophages [2]. In atherosclerosis, CRTAC1 is identified as a marker of atherosclerosis progression [4]. In lung adenocarcinoma, lower Crtac1 expression is associated with poor prognosis, and upregulation inhibits cell proliferation, invasion, and migration [3].
In conclusion, Crtac1 plays crucial roles in multiple disease processes such as cancer and degenerative diseases. Its function in modulating chemosensitivity in NSCLC, influencing tumor aggressiveness in urothelial and bladder cancers, contributing to spinal ligament degeneration, and being a marker in atherosclerosis and lung adenocarcinoma, reveals its significance in understanding disease mechanisms. Studies on Crtac1, especially through in vivo models, provide valuable insights into potential therapeutic strategies for these diseases.
References:
1. Jin, Zihui, Zhao, Lingling, Chang, Yixin, Huang, Chuanshu, Huang, Haishan. 2023. CRTAC1 enhances the chemosensitivity of non-small cell lung cancer to cisplatin by eliciting RyR-mediated calcium release and inhibiting Akt1 expression. In Cell death & disease, 14, 563. doi:10.1038/s41419-023-06088-1. https://pubmed.ncbi.nlm.nih.gov/37633993/
2. Tang, Yulong, Zhuo, Dachun, Yu, Yuexin, Chen, Yuanyuan, Liu, Jing. 2024. Single-cell RNA sequencing reveals the CRTAC1+ population actively contributes to the pathogenesis of spinal ligament degeneration by SPP1+ macrophage. In Aging cell, 23, e14320. doi:10.1111/acel.14320. https://pubmed.ncbi.nlm.nih.gov/39158018/
3. Tan, Lin, Zhang, Han, Ding, Yun, Huang, Yangyun, Sun, Daqiang. 2024. CRTAC1 identified as a promising diagnosis and prognostic biomarker in lung adenocarcinoma. In Scientific reports, 14, 11223. doi:10.1038/s41598-024-61804-x. https://pubmed.ncbi.nlm.nih.gov/38755183/
4. Mosquera, Jose Verdezoto, Auguste, Gaëlle, Wong, Doris, Sheffield, Nathan C, Miller, Clint L. 2023. Integrative single-cell meta-analysis reveals disease-relevant vascular cell states and markers in human atherosclerosis. In Cell reports, 42, 113380. doi:10.1016/j.celrep.2023.113380. https://pubmed.ncbi.nlm.nih.gov/37950869/
5. Li, Wei-Ming, Chan, Ti-Chun, Wei, Yu-Ching, Wang, Shao-Chuan, Yeh, Cheng-Fa. . Downregulation of CRTAC1 in Urothelial Carcinoma Promotes Tumor Aggressiveness and Confers Poor Prognosis. In Frontiers in bioscience (Landmark edition), 28, 217. doi:10.31083/j.fbl2809217. https://pubmed.ncbi.nlm.nih.gov/37796703/
6. Beugelink, J Wouter, Hóf, Henrietta, Janssen, Bert J C. 2024. CRTAC1 has a Compact β-propeller-TTR Core Stabilized by Potassium Ions. In Journal of molecular biology, 436, 168712. doi:10.1016/j.jmb.2024.168712. https://pubmed.ncbi.nlm.nih.gov/39029889/
7. Yang, Jianghua, Fan, Li, Liao, Xiaoxing, Cui, Gongjing, Hu, Hailong. . CRTAC1 (Cartilage acidic protein 1) inhibits cell proliferation, migration, invasion and epithelial-mesenchymal transition (EMT) process in bladder cancer by downregulating Yin Yang 1 (YY1) to inactivate the TGF-β pathway. In Bioengineered, 12, 9377-9389. doi:10.1080/21655979.2021.1974645. https://pubmed.ncbi.nlm.nih.gov/34818994/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen