C57BL/6NCya-Suclg2em1flox/Cya
Common Name:
Suclg2-flox
Product ID:
S-CKO-18019
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Suclg2-flox
Strain ID
CKOCMP-20917-Suclg2-B6N-VA
Gene Name
Product ID
S-CKO-18019
Gene Alias
D6Wsu120e; G-SCS; GTPSCS; SCS-betaG
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Suclg2em1flox/Cya mice (Catalog S-CKO-18019) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000204224
NCBI RefSeq
NM_011507
Target Region
Exon 3~4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Suclg2, the succinyl-coenzyme A (CoA) synthetase GDP-forming subunit β, is involved in important metabolic processes. It participates in the tricarboxylic acid cycle by catalyzing the conversion of succinyl-CoA to succinate, generating GTP in the process. This function is crucial for mitochondrial energy production and maintaining normal cellular metabolism [3].
In lung adenocarcinoma, deletion of Suclg2 upregulates the succinylation level of mitochondrial proteins, inhibits key metabolic enzymes, and dampens mitochondrial function. Suclg2 itself is succinylated on Lys93, enhancing its protein stability, promoting cell proliferation and tumorigenesis [1].
In regulatory dendritic cells (diffDCs), Suclg2-interference impairs the tolerogenic function of diffDCs, enhances NF-κB signaling and expression of inflammatory genes, indicating its role in maintaining the immunoregulatory function of diffDCs [2].
In pheochromocytoma and paraganglioma, germline variants in Suclg2 lead to absence of its protein, decrease in SDHB subunit of SDH, faulty assembly of complex II, aberrant respiration, and elevated succinate accumulation [3].
In prostate cancer, ADT-induced upregulation of Suclg2 is associated with NE differentiation, and knockdown of Suclg2 suppresses NE differentiation and tumor growth [4].
In rheumatoid arthritis, SUCLG2-deficient T cells differentiate into pro-inflammatory effector cells, and preventing tubulin acetylation in these cells can inhibit synovitis [5].
In Alzheimer's disease, a single-nucleotide polymorphism in SUCLG2 is associated with CSF Aβ1-42 levels and rate of cognitive decline, and it is also included in the diagnostic panel for mild cognitive impairment [6,7].
In conclusion, Suclg2 plays essential roles in multiple biological processes and diseases. Its functions in mitochondrial metabolism, immune regulation, and tumorigenesis are revealed through various loss-of-function experiments. These findings contribute to understanding the mechanisms of diseases such as cancer, immune-related disorders, and neurodegenerative diseases, potentially providing new targets for treatment.
References：
1. Hu, Qifan, Xu, Jing, Wang, Lei, Han, Tianyu, Wang, Jian-Bin. 2023. SUCLG2 Regulates Mitochondrial Dysfunction through Succinylation in Lung Adenocarcinoma. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 10, e2303535. doi:10.1002/advs.202303535. https://pubmed.ncbi.nlm.nih.gov/37904651/
2. Zhang, Xiaomin, Liu, Juan, Cheng, Yujie, Liu, Shuxun, Cao, Xuetao. 2023. Metabolic enzyme Suclg2 maintains tolerogenicity of regulatory dendritic cells diffDCs by suppressing Lactb succinylation. In Journal of autoimmunity, 138, 103048. doi:10.1016/j.jaut.2023.103048. https://pubmed.ncbi.nlm.nih.gov/37216870/
3. Hadrava Vanova, Katerina, Pang, Ying, Krobova, Linda, Yang, Chunzhang, Pacak, Karel. . Germline SUCLG2 Variants in Patients With Pheochromocytoma and Paraganglioma. In Journal of the National Cancer Institute, 114, 130-138. doi:10.1093/jnci/djab158. https://pubmed.ncbi.nlm.nih.gov/34415331/
4. Lin, Shian-Ren, Wen, Yu-Ching, Yeh, Hsiu-Lien, Chen, Wei-Yu, Liu, Yen-Nien. 2020. EGFR-upregulated LIFR promotes SUCLG2-dependent castration resistance and neuroendocrine differentiation of prostate cancer. In Oncogene, 39, 6757-6775. doi:10.1038/s41388-020-01468-9. https://pubmed.ncbi.nlm.nih.gov/32963351/
5. Wu, Bowen, Qiu, Jingtao, Zhao, Tuantuan V, Goronzy, Jörg J, Weyand, Cornelia M. . Succinyl-CoA Ligase Deficiency in Pro-inflammatory and Tissue-Invasive T Cells. In Cell metabolism, 32, 967-980.e5. doi:10.1016/j.cmet.2020.10.025. https://pubmed.ncbi.nlm.nih.gov/33264602/
6. Ramirez, Alfredo, van der Flier, Wiesje M, Herold, Christine, Becker, Tim, Nöthen, Markus M. 2014. SUCLG2 identified as both a determinator of CSF Aβ1-42 levels and an attenuator of cognitive decline in Alzheimer's disease. In Human molecular genetics, 23, 6644-58. doi:10.1093/hmg/ddu372. https://pubmed.ncbi.nlm.nih.gov/25027320/
7. Wang, Yuye, Sun, Yu, Wang, Yu, Wang, Yi, Peng, Dantao. 2023. Identification of novel diagnostic panel for mild cognitive impairment and Alzheimer's disease: findings based on urine proteomics and machine learning. In Alzheimer's research & therapy, 15, 191. doi:10.1186/s13195-023-01324-4. https://pubmed.ncbi.nlm.nih.gov/37925455/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen