C57BL/6NCya-Suclg1em1flox/Cya
Common Name
Suclg1-flox
Product ID
S-CKO-18029
Backgroud
C57BL/6NCya
Strain ID
CKOCMP-56451-Suclg1-B6N-VA
When using this mouse strain in a publication, please cite “Suclg1-flox Mouse (Catalog S-CKO-18029) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Suclg1-flox
Strain ID
CKOCMP-56451-Suclg1-B6N-VA
Gene Name
Product ID
S-CKO-18029
Gene Alias
1500000I01Rik, Sucla1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 6
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000064740
NCBI RefSeq
NM_019879
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Overview of Gene Research
Suclg1, encoding the alpha subunit of succinate-CoA ligase, is a key enzyme in the tricarboxylic acid cycle. It is involved in maintaining mitochondrial DNA (mtDNA) integrity and stability, and is associated with pathways related to mitochondrial function and metabolism [2,3,4]. Genetic models are valuable for studying its functions, as disruptions in Suclg1 can lead to various biological consequences.
Genetic depletion of Suclg1 significantly delays disease progression in mouse and humanized leukemia models. This indicates that Suclg1 promotes leukemia progression by restricting succinyl-CoA levels to suppress the succinylation of mitochondrial RNA polymerase (POLRMT), thus maintaining mtDNA transcription, mitochondrial biogenesis, and leukemia cell proliferation [1]. In addition, in a mouse model of cytokine storm syndrome, neutrophil-derived extracellular vesicles deliver miR-27a-3p which suppresses Suclg1 expression, leading to itaconate accumulation in macrophages and ameliorating the syndrome [5].
In conclusion, Suclg1 plays a crucial role in mitochondrial function, mainly through its impact on mtDNA transcription and mitochondrial biogenesis. Its function is closely related to leukemia progression and cytokine storm syndrome, as revealed by model-based research. Understanding Suclg1's role in these processes may provide new insights into treating related diseases.
References：
1. Yan, Weiwei, Xie, Chengmei, Sun, Sijun, Xu, Shuangnian, Wang, Yi-Ping. 2024. SUCLG1 restricts POLRMT succinylation to enhance mitochondrial biogenesis and leukemia progression. In The EMBO journal, 43, 2337-2367. doi:10.1038/s44318-024-00101-9. https://pubmed.ncbi.nlm.nih.gov/38649537/
2. El-Hattab, Ayman W, Craigen, William J, Scaglia, Fernando. 2017. Mitochondrial DNA maintenance defects. In Biochimica et biophysica acta. Molecular basis of disease, 1863, 1539-1555. doi:10.1016/j.bbadis.2017.02.017. https://pubmed.ncbi.nlm.nih.gov/28215579/
3. El-Hattab, Ayman W, Scaglia, Fernando. . Mitochondrial DNA depletion syndromes: review and updates of genetic basis, manifestations, and therapeutic options. In Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics, 10, 186-98. doi:10.1007/s13311-013-0177-6. https://pubmed.ncbi.nlm.nih.gov/23385875/
4. Chen, Yi-Ming, Chen, Wei, Xu, Yue, Shi, Jiaming, Wang, Dan. 2022. Novel compound heterozygous SUCLG1 variants may contribute to mitochondria DNA depletion syndrome-9. In Molecular genetics & genomic medicine, 10, e2010. doi:10.1002/mgg3.2010. https://pubmed.ncbi.nlm.nih.gov/35762302/
5. Kang, Haixia, Liu, Ting, Wang, Yuanyuan, Luo, Yan, Wang, Jing. 2024. Neutrophil-macrophage communication via extracellular vesicle transfer promotes itaconate accumulation and ameliorates cytokine storm syndrome. In Cellular & molecular immunology, 21, 689-706. doi:10.1038/s41423-024-01174-6. https://pubmed.ncbi.nlm.nih.gov/38745069/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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