C57BL/6JCya-Baz2bem1flox/Cya
Common Name:
Baz2b-flox
Product ID:
S-CKO-18034
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Baz2b-flox
Strain ID
CKOCMP-407823-Baz2b-B6J-VB
Gene Name
Product ID
S-CKO-18034
Gene Alias
5830435C13Rik; D2Ertd794e
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Baz2bem1flox/Cya mice (Catalog S-CKO-18034) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112550
NCBI RefSeq
NM_001001182
Target Region
Exon 4~5
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
BAZ2B, the bromodomain adjacent to zinc finger 2B, is a regulatory subunit of the ISWI (Imitation Switch) remodeling complex, engaging in nucleosome remodeling. It contains multiple domains such as the PHD, BRD, and TAM domains. BAZ2B is crucial in various biological processes and its malfunction is associated with different diseases [1].
Loss-of-function and haploinsufficiency of BAZ2B are linked to an autosomal dominant neurodevelopmental syndrome. Phenotypes associated with BAZ2B deficiency include developmental delay, intellectual disability, autism spectrum disorder, speech delay, behavioral abnormalities, seizures, vision-related issues, congenital heart defects, poor fetal growth, and dysmorphic features [2,5]. Ablation of Baz2b, the mouse orthologue of BAZ-2 in C. elegans, attenuates age-dependent body-weight gain and prevents cognitive decline in ageing mice, suggesting its role in healthy ageing [3]. In vivo CRISPR screening identified Baz2b as a regulator of mammalian liver regeneration, and specific inhibitors can accelerate liver healing [4].
In summary, BAZ2B plays essential roles in neurodevelopment, healthy ageing, and liver regeneration. Studies on BAZ2B knockout mouse models have significantly contributed to understanding its functions in these processes and the associated diseases, providing potential therapeutic targets for related disorders.
References:
1. Feng, Yingying, Chen, Sizhuo, Zhou, Mengqi, Min, Jinrong, Liu, Ke. 2022. Crystal structure of the BAZ2B TAM domain. In Heliyon, 8, e09873. doi:10.1016/j.heliyon.2022.e09873. https://pubmed.ncbi.nlm.nih.gov/35865993/
2. Sewani, Soha, Azamian, Mahshid S, Mendelsohn, Bryce A, Lalani, Seema R, Scott, Daryl A. 2023. Neurodevelopmental and other phenotypes recurrently associated with heterozygous BAZ2B loss-of-function variants. In American journal of medical genetics. Part A, 194, e63445. doi:10.1002/ajmg.a.63445. https://pubmed.ncbi.nlm.nih.gov/37872713/
3. Yuan, Jie, Chang, Si-Yuan, Yin, Shi-Gang, Jiang, Lubin, Cai, Shi-Qing. 2020. Two conserved epigenetic regulators prevent healthy ageing. In Nature, 579, 118-122. doi:10.1038/s41586-020-2037-y. https://pubmed.ncbi.nlm.nih.gov/32103178/
4. Jia, Yuemeng, Li, Lin, Lin, Yu-Hsuan, Ready, Joseph M, Zhu, Hao. 2022. In vivo CRISPR screening identifies BAZ2 chromatin remodelers as druggable regulators of mammalian liver regeneration. In Cell stem cell, 29, 372-385.e8. doi:10.1016/j.stem.2022.01.001. https://pubmed.ncbi.nlm.nih.gov/35090595/
5. Scott, Tiana M, Guo, Hui, Eichler, Evan E, Holder, Jimmy L, Scott, Daryl A. 2020. BAZ2B haploinsufficiency as a cause of developmental delay, intellectual disability, and autism spectrum disorder. In Human mutation, 41, 921-925. doi:10.1002/humu.23992. https://pubmed.ncbi.nlm.nih.gov/31999386/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen