C57BL/6JCya-Adcy7em1flox/Cya
Common Name:
Adcy7-flox
Product ID:
S-CKO-18035
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Adcy7-flox
Strain ID
CKOCMP-11513-Adcy7-B6J-VB
Gene Name
Product ID
S-CKO-18035
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Adcy7em1flox/Cya mice (Catalog S-CKO-18035) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000171456
NCBI RefSeq
NM_001037723
Target Region
Exon 5~8
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Adcy7, short for adenylate cyclase 7, is an enzyme that converts ATP to cAMP. It is involved in G protein-coupled receptor signaling pathways. Its function is crucial in various biological processes as it can influence cell growth, apoptosis, and immune responses [2]. Gene knockout models, like the ones used in research, can help reveal its role in specific biological functions and disease conditions.
In hepatocellular carcinoma (HCC), a genome-wide in vivo CRISPR screen identified ADCY7 as a novel immune modulator. The transmembrane protein ADCY7 translocates to the nucleus, where it acts as a transcription cofactor of CEBPA to induce CCL5 transcription, increasing CD8+ T cell infiltration and restraining HCC progression. Exosomes with high ADCY7 levels also suppress HCC tumor growth [1].
In acute myeloid leukemia (AML), inhibition of ADCY7 using an shRNA-encoding lentivirus system in human AML cells led to decreased cell growth, elevated apoptosis, and lower c-Myc expression, suggesting that ADCY7 supports AML development [2].
In a patient with congenital hyperinsulinism (CHI), compound heterozygous variants in ADCY7 were identified. Nuclease technology-mediated ADCY7 knockout in the RIN-m cell line led to increased insulin secretion and activation of the glucose-stimulated insulin secretion pathway [3].
In conclusion, Adcy7 plays essential roles in immune-related processes in HCC, development of AML, and insulin secretion in CHI. Gene knockout models have been instrumental in revealing these functions, providing potential therapeutic targets for these diseases.
References:
1. Chen, Jianan, Jiang, Youhai, Hou, Minghui, Wang, Hongyang, Fu, Jing. 2024. Nuclear translocation of plasma membrane protein ADCY7 potentiates T cell-mediated antitumour immunity in HCC. In Gut, 74, 128-140. doi:10.1136/gutjnl-2024-332902. https://pubmed.ncbi.nlm.nih.gov/39349007/
2. Li, Chunling, Xie, Jingjing, Lu, Zhigang, Hu, Xuemei, Zhang, Cheng Cheng. 2015. ADCY7 supports development of acute myeloid leukemia. In Biochemical and biophysical research communications, 465, 47-52. doi:10.1016/j.bbrc.2015.07.123. https://pubmed.ncbi.nlm.nih.gov/26220344/
3. Alhaidan, Yazeid, Christesen, Henrik Thybo, Lundberg, Elena, Balwi, Mohammed A Al, Brusgaard, Klaus. 2021. Nuclease technology ADCY7 Knockout Stimulates the Insulin Secretion Pathway Leading to Excessive Insulin Secretion. In Frontiers in endocrinology, 12, 657873. doi:10.3389/fendo.2021.657873. https://pubmed.ncbi.nlm.nih.gov/34177802/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen