C57BL/6JCya-Krt14em1flox/Cya
Common Name:
Krt14-flox
Product ID:
S-CKO-18046
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Krt14-flox
Strain ID
CKOCMP-16664-Krt14-B6J-VB
Gene Name
Product ID
S-CKO-18046
Gene Alias
CK-14; K14; Krt-1.14; Krt1-14
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Krt14em1flox/Cya mice (Catalog S-CKO-18046) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000007272
NCBI RefSeq
NM_016958
Target Region
Exon 2~7
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Krt14, short for Keratin 14, is a type I keratin that is crucial for maintaining the structural integrity of epithelial tissues. It is often expressed in basal cells of stratified epithelia and has been implicated in various cellular processes such as cell migration, differentiation, and tissue regeneration [2,3,4].
In triple-negative breast cancer (TNBC), EZH2-H3K27me3 mediated upregulation of Krt14 promotes peritoneal metastasis. Loss of Krt14 significantly reduces TNBC migration, invasion, and peritoneal metastasis, indicating its role in cancer metastasis [1]. In airway basal cells, Krt14-KO led to impaired differentiation into club and ciliated cells but enhanced clonogenicity. Krt14 bound the tumor suppressor stratifin, and its disruption reduced stratifin protein abundance and increased expression of the oncogene dNp63a during differentiation [2]. In psoriatic skin inflammation, KC-specific knockout of Atg5 using Krt14-Cre revealed the role of an autophagy-based unconventional secretion of HMGB1 in promoting cutaneous inflammation [3]. In salivary glands, loss of Atg5 in Krt14+ cells led to functional impairments via pyroptosis [4].
In conclusion, Krt14 plays essential roles in maintaining tissue homeostasis, cell differentiation, and disease progression. Gene knockout and conditional knockout mouse models have been instrumental in revealing its functions in diseases like TNBC, psoriasis, and salivary gland diseases, highlighting its potential as a therapeutic target in these conditions.
References:
1. Verma, Ayushi, Singh, Akhilesh, Singh, Manish Pratap, Singh, Anup Kumar, Datta, Dipak. 2022. EZH2-H3K27me3 mediated KRT14 upregulation promotes TNBC peritoneal metastasis. In Nature communications, 13, 7344. doi:10.1038/s41467-022-35059-x. https://pubmed.ncbi.nlm.nih.gov/36446780/
2. Ievlev, Vitaly, Lynch, Thomas J, Freischlag, Kyle W, Engelhardt, John F, Parekh, Kalpaj R. 2023. Krt14 and Krt15 differentially regulate regenerative properties and differentiation potential of airway basal cells. In JCI insight, 8, . doi:10.1172/jci.insight.162041. https://pubmed.ncbi.nlm.nih.gov/36512409/
3. Wang, Zhen, Zhou, Hong, Zheng, Huaping, Li, Wei, Li, Jiong. 2020. Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin inflammation. In Autophagy, 17, 529-552. doi:10.1080/15548627.2020.1725381. https://pubmed.ncbi.nlm.nih.gov/32019420/
4. Yu, Siqi, Wang, Haisheng, Liu, Ming, Li, Qiuhui, Chen, Zhi. . Loss of ATG5 in KRT14+ cells leads to accumulated functional impairments of salivary glands via pyroptosis. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, e22631. doi:10.1096/fj.202200946R. https://pubmed.ncbi.nlm.nih.gov/36342387/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen