C57BL/6JCya-Pkd2l1em1flox/Cya
Common Name:
Pkd2l1-flox
Product ID:
S-CKO-18051
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pkd2l1-flox
Strain ID
CKOCMP-329064-Pkd2l1-B6J-VB
Gene Name
Product ID
S-CKO-18051
Gene Alias
B830002B15; PCL; PKD2L; Pkdl; TRPP3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
19
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pkd2l1em1flox/Cya mice (Catalog S-CKO-18051) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000042026
NCBI RefSeq
NM_181422.3
Target Region
Exon 3~4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Pkd2l1, also known as TRPP2 or TRPP3, is a member of the transient receptor potential family and forms ciliary ion channels [1,3,5,8,9,10]. It is involved in multiple biological processes. In neurons, it may be related to brain electrical signaling and in spinal cord, it is associated with sensory functions of cerebrospinal fluid-contacting neurons (CSF-cNs) [1,2]. It also participates in processes like hedgehog signaling, intestinal development, and sour tasting [8]. Genetic models, especially mouse models, have been crucial in studying its functions.
Loss of Pkd2l1 expression in mice leads to ablation of primary ciliary maturation and attenuation of neuronal high-frequency excitability in hippocampal neurons, resulting in seizure susceptibility and autism spectrum disorder-like behavior, suggesting it is a regulator of hippocampal excitability [1]. In zebrafish, loss of Pkd2l1 nearly abolishes CSF-cN calcium activity and single channel opening, and adult pkd2l1 mutant zebrafish develop an exaggerated spine curvature, indicating its role in mechanoception in CSF-cNs and maintenance of spine curvature [4]. In mouse spinal cords, Pkd2l1+ CSF-cNs have neural stem cell properties both in vitro and in vivo, and their activation and proliferation can be induced by spinal cord injury, suggesting a potential role in spinal cord injury recovery [6,7].
In conclusion, Pkd2l1 plays essential roles in neuronal excitability regulation, mechanoception in CSF-cNs, and maintenance of spine curvature. The study of Pkd2l1 knockout mouse models has provided valuable insights into its functions and its potential involvement in neurological and spinal-related diseases, such as autism-like behaviors and spine curvature disorders.
References:
1. Vien, Thuy N, Ta, My C, Kimura, Louise F, Onay, Tuncer, DeCaen, Paul G. 2023. Primary cilia TRP channel regulates hippocampal excitability. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2219686120. doi:10.1073/pnas.2219686120. https://pubmed.ncbi.nlm.nih.gov/37216541/
2. Liu, Xiaohe, Rich, Karen, Nasseri, Sohail M, Svenningsen, Åsa, Zhang, Mengliang. 2023. A Comparison of PKD2L1-Expressing Cerebrospinal Fluid Contacting Neurons in Spinal Cords of Rodents, Carnivores, and Primates. In International journal of molecular sciences, 24, . doi:10.3390/ijms241713582. https://pubmed.ncbi.nlm.nih.gov/37686387/
3. Su, Qiang, Chen, Mengying, Wang, Yan, Yu, Yong, Shi, Yigong. 2021. Structural basis for Ca2+ activation of the heteromeric PKD1L3/PKD2L1 channel. In Nature communications, 12, 4871. doi:10.1038/s41467-021-25216-z. https://pubmed.ncbi.nlm.nih.gov/34381056/
4. Sternberg, Jenna R, Prendergast, Andrew E, Brosse, Lucie, Delmas, Patrick, Wyart, Claire. 2018. Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature. In Nature communications, 9, 3804. doi:10.1038/s41467-018-06225-x. https://pubmed.ncbi.nlm.nih.gov/30228263/
5. Park, Eunice Yon June, Baik, Julia Young, Kwak, Misun, So, Insuk. 2019. The role of calmodulin in regulating calcium-permeable PKD2L1 channel activity. In The Korean journal of physiology & pharmacology : official journal of the Korean Physiological Society and the Korean Society of Pharmacology, 23, 219-227. doi:10.4196/kjpp.2019.23.3.219. https://pubmed.ncbi.nlm.nih.gov/31080352/
6. Cao, Liang, Huang, Ming-Zhi, Zhang, Qiang, Dou, Xiao-Wei, Li, Qing. 2022. The neural stem cell properties of Pkd2l1+ cerebrospinal fluid-contacting neurons in vivo. In Frontiers in cellular neuroscience, 16, 992520. doi:10.3389/fncel.2022.992520. https://pubmed.ncbi.nlm.nih.gov/36159391/
7. Wang, Shuo, He, Yuqi, Zhang, Huiqian, Dou, Xiaowei, Li, Qing. 2021. The Neural Stem Cell Properties of PKD2L1+ Cerebrospinal Fluid-Contacting Neurons in vitro. In Frontiers in cellular neuroscience, 15, 630882. doi:10.3389/fncel.2021.630882. https://pubmed.ncbi.nlm.nih.gov/33790741/
8. Zheng, Wang, Hussein, Shaimaa, Yang, JungWoo, Tang, Jingfeng, Chen, Xing-Zhen. 2015. A novel PKD2L1 C-terminal domain critical for trimerization and channel function. In Scientific reports, 5, 9460. doi:10.1038/srep09460. https://pubmed.ncbi.nlm.nih.gov/25820328/
9. Chen, Peihua, Wu, Jin-zhi, Zhao, Jie, Yang, Wei, Liu, Xiao-dong. 2015. PKD2L1/PKD1L3 channel complex with an alkali-activated mechanism and calcium-dependent inactivation. In European biophysics journal : EBJ, 44, 483-92. doi:10.1007/s00249-015-1040-y. https://pubmed.ncbi.nlm.nih.gov/26066678/
10. Ng, Leo C T, Vien, Thuy N, Yarov-Yarovoy, Vladimir, DeCaen, Paul G. 2019. Opening TRPP2 (PKD2L1) requires the transfer of gating charges. In Proceedings of the National Academy of Sciences of the United States of America, 116, 15540-15549. doi:10.1073/pnas.1902917116. https://pubmed.ncbi.nlm.nih.gov/31315976/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen