C57BL/6JCya-Nmur1em1flox/Cya
Common Name:
Nmur1-flox
Product ID:
S-CKO-18057
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Nmur1-flox
Strain ID
CKOCMP-14767-Nmur1-B6J-VB
Gene Name
Product ID
S-CKO-18057
Gene Alias
FM-3; Gpr66; NMU-R1; NMU1R; NmU-R
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nmur1em1flox/Cya mice (Catalog S-CKO-18057) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000212058
NCBI RefSeq
NM_001319227
Target Region
Exon 2~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Nmur1, the neuromedin U receptor 1, is a G-protein-coupled receptor. Neuromedin U (NMU) binding to NMUR1 activates downstream signaling, which is involved in multiple biological processes such as mucosal immunity, allergic inflammation, and bone remodeling. The study of Nmur1-related functions often relies on genetic models like knockout (KO) mouse models to understand its roles in vivo [3,4,5,6].
In mice, a subset of small intestine eosinophils expresses NMUR1. Fate-mapping analyses show local microenvironment programming of its expression in these eosinophils, and inflammation further enhances it. Genetic perturbation experiments reveal that NMU-mediated activation of eosinophils via NMUR1 promotes goblet cell differentiation, highlighting its role in epithelial cell differentiation and barrier immunity in the small intestine [1]. In ILC2-driven allergic lung inflammation, Nmur1 is preferentially expressed by ILC2s at steady state and after IL-25 stimulation. Loss of NMU-NMUR1 signaling reduces ILC2 frequency and effector function, and alters transcriptional programs following allergen challenge, suggesting its importance in allergic inflammation at mucosal surfaces [2]. In bone remodeling, knockdown of Nmur1 in MC3T3-E1 cells in vitro shows it is required for NMU-dependent signaling in these cells, but global knockout of Nmur1 in 12-week-old male mice shows no change in trabecular bone volume, indicating it may not be essential for NMU-mediated bone remodeling in vivo [3].
In conclusion, Nmur1 is crucial for regulating mucosal immunity, allergic inflammation, and potentially bone remodeling. KO mouse models have been instrumental in uncovering these functions, highlighting its significance in understanding immune-related and bone-related disease mechanisms.
References:
1. Li, Yu, Liu, Shaorui, Zhou, Kewen, He, Danyang, Xu, Heping. 2023. Neuromedin U programs eosinophils to promote mucosal immunity of the small intestine. In Science (New York, N.Y.), 381, 1189-1196. doi:10.1126/science.ade4177. https://pubmed.ncbi.nlm.nih.gov/37708282/
2. Wallrapp, Antonia, Riesenfeld, Samantha J, Burkett, Patrick R, Regev, Aviv, Kuchroo, Vijay K. 2017. The neuropeptide NMU amplifies ILC2-driven allergic lung inflammation. In Nature, 549, 351-356. doi:10.1038/nature24029. https://pubmed.ncbi.nlm.nih.gov/28902842/
3. Hsiao, Yu-Tin, Manikowski, Kelli J, Snyder, Samantha, Allen, Matthew R, Lowery, Jonathan W. 2021. NMUR1 in the NMU-Mediated Regulation of Bone Remodeling. In Life (Basel, Switzerland), 11, . doi:10.3390/life11101028. https://pubmed.ncbi.nlm.nih.gov/34685399/
4. Tsou, Amy M, Yano, Hiroshi, Parkhurst, Christopher N, Lira, Sergio A, Artis, David. 2022. Neuropeptide regulation of non-redundant ILC2 responses at barrier surfaces. In Nature, 611, 787-793. doi:10.1038/s41586-022-05297-6. https://pubmed.ncbi.nlm.nih.gov/36323781/
5. Cardoso, Vânia, Chesné, Julie, Ribeiro, Hélder, Harris, Nicola, Veiga-Fernandes, Henrique. 2017. Neuronal regulation of type 2 innate lymphoid cells via neuromedin U. In Nature, 549, 277-281. doi:10.1038/nature23469. https://pubmed.ncbi.nlm.nih.gov/28869974/
6. Jarick, Katja J, Topczewska, Patrycja M, Jakob, Manuel O, Artis, David, Klose, Christoph S N. 2022. Non-redundant functions of group 2 innate lymphoid cells. In Nature, 611, 794-800. doi:10.1038/s41586-022-05395-5. https://pubmed.ncbi.nlm.nih.gov/36323785/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen