C57BL/6JCya-Mpc2em1flox/Cya
Common Name:
Mpc2-flox
Product ID:
S-CKO-18069
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mpc2-flox
Strain ID
CKOCMP-70456-Mpc2-B6J-VB
Gene Name
Product ID
S-CKO-18069
Gene Alias
0610006C01Rik; 2010002I07Rik; 2610205H19Rik; Brp44; ESTM43
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mpc2em1flox/Cya mice (Catalog S-CKO-18069) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027853
NCBI RefSeq
NM_027430
Target Region
Exon 3~4
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
Mpc2 is a subunit of the mitochondrial pyruvate carrier (MPC) hetero-oligomeric complex, along with Mpc1. The MPC complex is crucial as it transports pyruvate, a product of glycolysis in the cytosol, across the inner mitochondrial membrane into the mitochondrial matrix. This process links cytosolic and mitochondrial metabolism, being essential for processes like the tricarboxylic acid cycle, generation of reducing equivalents and ATP, and biosynthesis of glucose, fatty acids, and amino acids [2,3,4].
Genetic ablation of Mpc2 has shown significant impacts. In adult murine hearts, genetic ablation of the MPC (including Mpc2) was sufficient to induce hypertrophy and heart failure, indicating its importance in cardiac function [1]. In hepatocyte-specific Mpc2 knockout (LS-Mpc2-/-) mice, there was reduced phosphorylation of the rate-limiting enzyme in branched chain amino acid (BCAA) catabolism, along with activation of mTOR signaling, demonstrating a role in BCAA metabolism [5]. In mice, genetic inactivation of Mpc2 led to basal cell hyperplasia in the tracheal epithelium, reduced ciliated cells during homeostasis, and delayed epithelial regeneration following injury, highlighting its role in airway basal progenitor cell function [7]. In addition, knockdown of Mpc2 in cultured HK2 cells exacerbated cisplatin-induced acute kidney injury, while overexpression attenuated the nephrotoxicity, showing its significance in kidney protection [6].
In conclusion, Mpc2 plays essential roles in multiple biological processes. Through gene knockout studies in mouse models, it has been revealed to be crucial in cardiac function, BCAA metabolism, airway basal progenitor cell function, and kidney protection. Understanding Mpc2's function provides insights into the pathogenesis of related diseases such as heart failure, metabolic disorders, and airway and kidney diseases, potentially guiding the development of new therapeutic strategies.
References:
1. Cluntun, Ahmad A, Badolia, Rachit, Lettlova, Sandra, Rutter, Jared, Drakos, Stavros G. 2020. The pyruvate-lactate axis modulates cardiac hypertrophy and heart failure. In Cell metabolism, 33, 629-648.e10. doi:10.1016/j.cmet.2020.12.003. https://pubmed.ncbi.nlm.nih.gov/33333007/
2. Pujol, Claire, Lebigot, Elise, Gaignard, Pauline, Wai, Timothy, Slama, Abdelhamid. . MPC2 variants disrupt mitochondrial pyruvate metabolism and cause an early-onset mitochondriopathy. In Brain : a journal of neurology, 146, 858-864. doi:10.1093/brain/awac444. https://pubmed.ncbi.nlm.nih.gov/36417180/
3. Tavoulari, Sotiria, Sichrovsky, Maximilian, Kunji, Edmund R S. 2023. Fifty years of the mitochondrial pyruvate carrier: New insights into its structure, function, and inhibition. In Acta physiologica (Oxford, England), 238, e14016. doi:10.1111/apha.14016. https://pubmed.ncbi.nlm.nih.gov/37366179/
4. Yiew, Nicole K H, Finck, Brian N. 2022. The mitochondrial pyruvate carrier at the crossroads of intermediary metabolism. In American journal of physiology. Endocrinology and metabolism, 323, E33-E52. doi:10.1152/ajpendo.00074.2022. https://pubmed.ncbi.nlm.nih.gov/35635330/
5. Ferguson, Daniel, Eichler, Sophie J, Yiew, Nicole K H, Niemi, Natalie M, Finck, Brian N. 2023. Mitochondrial pyruvate carrier inhibition initiates metabolic crosstalk to stimulate branched chain amino acid catabolism. In Molecular metabolism, 70, 101694. doi:10.1016/j.molmet.2023.101694. https://pubmed.ncbi.nlm.nih.gov/36801448/
6. Wu, Lin, Li, Qing, Lu, Fang, Mao, Huijuan, Yuan, Yanggang. 2024. Mitochondrial pyruvate carrier 2 mitigates acute kidney injury via sustaining mitochondrial metabolism. In International journal of biological sciences, 20, 4551-4565. doi:10.7150/ijbs.98627. https://pubmed.ncbi.nlm.nih.gov/39247825/
7. Li, Yawen, He, Yalin, Zheng, Qi, Ren, Tao, Sui, Pengfei. 2024. Mitochondrial pyruvate carriers control airway basal progenitor cell function through glycolytic-epigenetic reprogramming. In Cell stem cell, 32, 105-120.e6. doi:10.1016/j.stem.2024.09.015. https://pubmed.ncbi.nlm.nih.gov/39426380/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen