C57BL/6JCya-Igfbp4em1flox/Cya
Common Name:
Igfbp4-flox
Product ID:
S-CKO-18089
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Igfbp4-flox
Strain ID
CKOCMP-16010-Igfbp4-B6J-VB
Gene Name
Product ID
S-CKO-18089
Gene Alias
Deb2; IBP-4; IGFBP-4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Igfbp4em1flox/Cya mice (Catalog S-CKO-18089) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000017637
NCBI RefSeq
NM_010517
Target Region
Exon 2~3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Igfbp4, Insulin-like growth factor binding protein 4, is highly expressed in adipocytes and osteoblasts. It is an inhibitor of IGFs in vitro and is involved in the regulation of IGF-I signaling pathway, which has wide-ranging effects on cell growth, differentiation, and metabolism [3].
In Igfbp4 null mice (Igfbp4-/-), inguinal and gonadal white adipose tissue had decreased weights and Pparγ expression. Cultures of primary bone marrow stromal cells and ear mesenchymal stem cells from these mutant mice showed reduced adipogenesis. Also, there were sexual differences in adipocyte expansion on a high-fat diet, indicating that Igfbp4 is required for inguinal fat expansion in female mice but not in male mice [3]. In addition, in type 2 diabetes (T2D) patients, higher levels of total and intact IGFBP4 were associated with all-cause mortality, suggesting its role in T2D-related mortality [1]. In chronic lymphocytic leukemia (CLL), IGFBP4 was identified as an independent prognostic factor, where high IGFBP4 risk scores were associated with a high incidence of trisomy 12q [2].
In conclusion, Igfbp4 plays essential roles in adipogenesis and the distribution of adipose depots, as revealed by Igfbp4-/-mouse models. It also has implications in diseases such as T2D and CLL, highlighting its significance in understanding the pathophysiology of these conditions.
References:
1. Gude, Mette Faurholdt, Hjortebjerg, Rikke, Bjerre, Mette, Sandbæk, Annelli, Frystyk, Jan. 2023. The STC2-PAPP-A-IGFBP4-IGF1 axis and its associations to mortality and CVD in T2D. In Endocrine connections, 12, . doi:10.1530/EC-22-0451. https://pubmed.ncbi.nlm.nih.gov/36607154/
2. Shi, Yuye, Xu, Xiaohu, He, Zhengmei, Wang, Chunling, Yu, Liang. 2023. AKAP12 and IGFBP4 Are Prognostic Factors for Chronic Lymphocytic Leukemia. In Acta haematologica, 146, 474-481. doi:10.1159/000530618. https://pubmed.ncbi.nlm.nih.gov/37605556/
3. Maridas, David E, DeMambro, Victoria E, Le, Phuong T, Mohan, Subburaman, Rosen, Clifford J. . IGFBP4 Is Required for Adipogenesis and Influences the Distribution of Adipose Depots. In Endocrinology, 158, 3488-3500. doi:10.1210/en.2017-00248. https://pubmed.ncbi.nlm.nih.gov/28938423/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen