C57BL/6JCya-Pdzk1em1flox/Cya
Common Name:
Pdzk1-flox
Product ID:
S-CKO-18105
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Pdzk1-flox
Strain ID
CKOCMP-59020-Pdzk1-B6J-VB
Gene Name
Product ID
S-CKO-18105
Gene Alias
1700023D20Rik; 2610507N21Rik; 4921513F16Rik; D3Ertd537e; NHERF-3; Nherf3; Pdzd1; mPDZK1; naPi-Cap1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Pdzk1em1flox/Cya mice (Catalog S-CKO-18105) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107070
NCBI RefSeq
NM_001146001
Target Region
Exon 3
Size of Effective Region
~0.7 kb
Detailed Document
Overview of Gene Research
PDZK1, belonging to the Na+/H+ Exchanger (NHE) regulatory factor family, is a PDZ protein. It plays crucial roles in multiple biological processes and disease-related pathways. PDZK1 has been shown to be involved in pathways such as those related to mitochondrial function, epidermal growth factor receptor (EGFR) signaling, and platelet-derived growth factor receptor-β (PDGFR-β) signaling, with implications for diseases like osteoarthritis (OA), triple-negative breast cancer (TNBC), and clear cell renal cell carcinoma (ccRCC) [1,2,3].
In OA, Pdzk1 knockout in chondrocytes exacerbates mechanical overload-induced cartilage degeneration, indicating its protective role against mechanical overload-induced chondrocyte senescence and OA by targeting mitochondrial function [1].
In TNBC, PDZK1 downregulation is related to erlotinib resistance. PDZK1 binds to EGFR, promoting its degradation and inhibiting phosphorylation, and its restoration can reverse the degree of cell malignancy [2].
In ccRCC, PDZK1 is significantly downregulated in sunitinib-resistant specimens, and it negatively regulates PDGFR-β phosphorylation and downstream pathways, with miR-15b antagomirs enhancing sunitinib cytotoxicity by upregulating PDZK1 [3].
In conclusion, PDZK1 is essential in various biological functions and disease-related pathways. Gene knockout studies in mouse models, such as in OA, TNBC, and ccRCC, have revealed its key roles in disease development and response to treatment. These findings highlight PDZK1 as a potential therapeutic target for these diseases.
References：
1. Shao, Yan, Zhang, Hongbo, Guan, Hong, Bai, Xiaochun, Cai, Daozhang. 2024. PDZK1 protects against mechanical overload-induced chondrocyte senescence and osteoarthritis by targeting mitochondrial function. In Bone research, 12, 41. doi:10.1038/s41413-024-00344-6. https://pubmed.ncbi.nlm.nih.gov/39019845/
2. Ma, Yuanzhen, Fang, Zhiyu, Zhang, Hongning, Mao, Yuke, Zheng, Junfang. 2024. PDZK1 suppresses TNBC development and sensitizes TNBC cells to erlotinib via the EGFR pathway. In Cell death & disease, 15, 199. doi:10.1038/s41419-024-06502-2. https://pubmed.ncbi.nlm.nih.gov/38604999/
3. Wang, Haibo, Zhang, Lijie, Liu, Hua, Bai, Tao, He, Junqi. 2024. PDZK1 confers sensitivity to sunitinib in clear cell renal cell carcinoma by suppressing the PDGFR-β pathway. In British journal of cancer, 131, 347-360. doi:10.1038/s41416-024-02725-4. https://pubmed.ncbi.nlm.nih.gov/38822145/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen