C57BL/6JCya-Acap1em1flox/Cya
Common Name:
Acap1-flox
Product ID:
S-CKO-18114
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Acap1-flox
Strain ID
CKOCMP-216859-Acap1-B6J-VB
Gene Name
Product ID
S-CKO-18114
Gene Alias
Centb1
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Acap1em1flox/Cya mice (Catalog S-CKO-18114) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001631
NCBI RefSeq
NM_153788.3
Target Region
Exon 3~10
Size of Effective Region
~3.0 kb
Detailed Document
Overview of Gene Research
ACAP1, short for Arfgap with Coil-coil, Ankryin repeat, and PH domain I, is an Arf6 GTPase-activating protein (GAP) that plays a crucial role in endocytic recycling [3,4,5,6]. It is involved in sorting cargoes at the recycling endosome for their return to the plasma membrane by recognizing recycling sorting signals, which is essential for normal cellular function [4,6]. It has also been associated with pathways related to cell migration and G protein-coupled receptor trafficking [2,3,5].
In solid tumors, ACAP1 deficiency predicts inferior immunotherapy response. ACAP1 is highly expressed in immune-related tissues and cells, especially in tumor-infiltrating lymphocytes (TILs). Its expression is negatively regulated by promoter DNA methylation, and SPI1 positively regulates its expression in immune cells. ACAP1 levels positively correlate with TIL infiltrating levels, especially CD8+ T cells. Functionally, depletion of ACAP1 by RNA interference significantly impairs T cell-mediated killing of tumor cells, indicating its importance for normal TIL function [1]. In ovarian cancer, high ACAP1 expression predicts poor prognosis, and its expression and methylation status are strongly correlated with immune infiltration levels, immunomodulators, and chemokines [3]. In lung adenocarcinoma, low ACAP1 expression is correlated with unsatisfactory overall and disease-specific survival, and overexpression of ACAP1 attenuates cell proliferation, migration, and invasion while promoting apoptosis [5].
In conclusion, ACAP1 is essential for endocytic recycling and normal TIL function. Its deficiency is associated with poor prognosis and immunotherapy response in multiple cancer types. The study of ACAP1 through functional studies, especially those related to its deficiency, helps to understand its role in tumor immunity and provides potential biomarkers for cancer prognosis and immunotherapy response prediction.
References:
1. Yi, Qiyi, Pu, Youguang, Chao, Fengmei, Bian, Po, Lv, Lei. 2022. ACAP1 Deficiency Predicts Inferior Immunotherapy Response in Solid Tumors. In Cancers, 14, . doi:10.3390/cancers14235951. https://pubmed.ncbi.nlm.nih.gov/36497434/
2. Xu, Rongmei, Wan, Min, Shi, Xuemeng, Yi, Ping, Zhang, Rongying. 2023. A Rab10-ACAP1-Arf6 GTPases cascade modulates M4 muscarinic acetylcholine receptor trafficking and signaling. In Cellular and molecular life sciences : CMLS, 80, 87. doi:10.1007/s00018-023-04722-x. https://pubmed.ncbi.nlm.nih.gov/36917255/
3. Zhang, Jiawen, Zhang, Qinyi, Zhang, Jing, Wang, Qingying. 2020. Expression of ACAP1 Is Associated with Tumor Immune Infiltration and Clinical Outcome of Ovarian Cancer. In DNA and cell biology, 39, 1545-1557. doi:10.1089/dna.2020.5596. https://pubmed.ncbi.nlm.nih.gov/32456571/
4. Li, Jian, Hsu, Victor W. 2015. An ACAP1 coat complex acting in endocytic recycling. In Methods in cell biology, 130, 81-99. doi:10.1016/bs.mcb.2015.03.019. https://pubmed.ncbi.nlm.nih.gov/26360030/
5. Wang, Ning, Zhu, Lingye, Xu, Xiaomei, Yu, Chang, Huang, Xiaoying. 2022. Integrated analysis and validation reveal ACAP1 as a novel prognostic biomarker associated with tumor immunity in lung adenocarcinoma. In Computational and structural biotechnology journal, 20, 4390-4401. doi:10.1016/j.csbj.2022.08.026. https://pubmed.ncbi.nlm.nih.gov/36051873/
6. Dai, Jun, Li, Jian, Bos, Erik, Peters, Peter J, Hsu, Victor W. . ACAP1 promotes endocytic recycling by recognizing recycling sorting signals. In Developmental cell, 7, 771-6. doi:. https://pubmed.ncbi.nlm.nih.gov/15525538/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen