C57BL/6JCya-Pmaip1em1flox/Cya
Common Name
Pmaip1-flox
Product ID
S-CKO-18133
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-58801-Pmaip1-B6J-VB
When using this mouse strain in a publication, please cite “Pmaip1-flox Mouse (Catalog S-CKO-18133) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Pmaip1-flox
Strain ID
CKOCMP-58801-Pmaip1-B6J-VB
Gene Name
Product ID
S-CKO-18133
Gene Alias
Noxa
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 18
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000025399
NCBI RefSeq
NM_021451.2
Target Region
Exon 2~3
Size of Effective Region
~3.5 kb
Overview of Gene Research
Pmaip1, also known as NoxA, is a pro-apoptotic mitochondrial protein in the B-cell lymphoma-2 (Bcl-2) family. It plays a role in apoptosis, and is involved in various biological processes and signaling pathways. Its dysregulation can have significant impacts on cell survival and disease development [3,4,6,7].
In osteoporosis, Pmaip1 was identified as a core gene. It can promote the osteogenesis, migration and proliferation of bone marrow mesenchymal stem cells (BMSCs), and was thus proposed as a potential biomarker for osteoporosis diagnosis and treatment [1]. In another study, Pmaip1 was found to suppress autophagy in osteoblasts via the AMPK/mTOR pathway, inhibiting their proliferation and differentiation, and si-PMAIP1 could inhibit the development of osteoporosis in an ovariectomy (OVX) model of rats [2].
In acute myeloid leukemia (AML), Pmaip1 knockout confirmed its major role in driving the synergy between venetoclax and 5-azacitidine, suggesting it may be a key factor in the response to this combinatorial treatment [4].
In follicular thyroid carcinoma (FTC), knockdown of Pmaip1 significantly inhibited the proliferation and metastasis of FTC cells both in vivo and in vitro, as it regulated FOSL1 by modulating the Wnt signaling pathway [5].
In acute aortic dissection (AAD), the pro-apoptotic gene Pmaip1 was highly expressed, and cannabidiol could inhibit its expression to reduce AAD-associated morbidity and mortality by suppressing macrophage infiltration and PMAIP1-induced vascular smooth muscle cell apoptosis [6].
In Alzheimer's disease, siRNA-PMAIP1 could alleviate mitochondrial dysfunction and inhibit cell apoptosis, indicating it may affect mitochondrial function in hippocampal neuronal cells [8].
In conclusion, Pmaip1 is a crucial gene involved in apoptosis and various biological processes. Studies using gene-knockout or other loss-of-function models have revealed its significant roles in diseases such as osteoporosis, AML, FTC, AAD, and Alzheimer's disease, providing valuable insights into disease mechanisms and potential therapeutic targets.
References：
1. Li, Tao, Yuan, Jinghong, Xu, Peichuan, Wu, Tianlong, Cheng, Xigao. 2024. PMAIP1, a novel diagnostic and potential therapeutic biomarker in osteoporosis. In Aging, 16, 3694-3715. doi:10.18632/aging.205553. https://pubmed.ncbi.nlm.nih.gov/38372699/
2. Gao, Yijie, Huang, Anquan, Zhao, Yantao, Du, Yunxia. 2024. PMAIP1 regulates autophagy in osteoblasts via the AMPK/mTOR pathway in osteoporosis. In Human cell, 37, 1024-1038. doi:10.1007/s13577-024-01067-w. https://pubmed.ncbi.nlm.nih.gov/38691334/
3. Zhao, Yongxia, Zhao, Changbin, Deng, Yuelin, Zhang, Dexiang, Li, Hongmei. 2024. PMAIP1 promotes J subgroup avian leukosis virus replication by regulating mitochondrial function. In Poultry science, 103, 103617. doi:10.1016/j.psj.2024.103617. https://pubmed.ncbi.nlm.nih.gov/38547674/
4. Jin, Sha, Cojocari, Dan, Purkal, Julie J, Leverson, Joel D, Phillips, Darren C. 2020. 5-Azacitidine Induces NOXA to Prime AML Cells for Venetoclax-Mediated Apoptosis. In Clinical cancer research : an official journal of the American Association for Cancer Research, 26, 3371-3383. doi:10.1158/1078-0432.CCR-19-1900. https://pubmed.ncbi.nlm.nih.gov/32054729/
5. Wang, Haobo, Shang, Fangjian, Wang, Yifang, Liu, Bo, Zhao, Zengren. 2025. PMAIP1 regulates the progression of follicular thyroid carcinoma through the Wnt3/FOSL1 pathway. In Frontiers in oncology, 15, 1502391. doi:10.3389/fonc.2025.1502391. https://pubmed.ncbi.nlm.nih.gov/39944827/
6. Guo, Yilong, Che, Yang, Zhang, Xuelin, Wang, Li, Guo, Wei. 2024. Cannabidiol protects against acute aortic dissection by inhibiting macrophage infiltration and PMAIP1-induced vascular smooth muscle cell apoptosis. In Journal of molecular and cellular cardiology, 189, 38-51. doi:10.1016/j.yjmcc.2024.02.006. https://pubmed.ncbi.nlm.nih.gov/38387723/
7. Miura, Rika, Mimura, Imari, Saigusa, Hanako, Tanaka, Tetsuhiro, Nangaku, Masaomi. 2023. Chromatin remodeling factor, INO80, inhibits PMAIP1 in renal tubular cells via exchange of histone variant H2A.Z. for H2A. In Scientific reports, 13, 13235. doi:10.1038/s41598-023-40540-8. https://pubmed.ncbi.nlm.nih.gov/37580530/
8. Ling, Yingchun, Hu, Lingmin, Chen, Jie, Zhao, Mingyong, Dai, Xinyang. 2024. The mechanism of mitochondrial metabolic gene PMAIP1 involved in Alzheimer's disease process based on bioinformatics analysis and experimental validation. In Clinics (Sao Paulo, Brazil), 79, 100373. doi:10.1016/j.clinsp.2024.100373. https://pubmed.ncbi.nlm.nih.gov/38692009/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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