C57BL/6JCya-Robo3em1flox/Cya
Common Name
Robo3-flox
Product ID
S-CKO-18151
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-19649-Robo3-B6J-VB
Status
When using this mouse strain in a publication, please cite “Robo3-flox Mouse (Catalog S-CKO-18151) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Robo3-flox
Strain ID
CKOCMP-19649-Robo3-B6J-VB
Gene Name
Product ID
S-CKO-18151
Gene Alias
Rig1, Rbig1, Rig-1, Robo3a, Robo3b
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 9 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000115038
NCBI Transcript ID
NM_001164767
Target Region
Exon 2~3
Size of Effective Region
~1.7 kb
Overview of Gene Research
Robo3, a member of the Roundabout (ROBO) gene family, is an unconventional Robo receptor. It plays crucial roles in axon crossing of the CNS midline, especially in the development of commissural axons that connect both sides of the nervous system. It is associated with the SLIT/ROBO signaling pathway, which is important for the nervous system and embryonic development [1,3].
Many studies using transgenic mouse models have shown that Robo3 expression is mandatory for commissural axon guidance to the floor plate [1]. Mutations in human ROBO3 are responsible for horizontal gaze palsy with progressive scoliosis (HGPPS), an autosomal recessive disorder. Patients with HGPPS display midline crossing defects, including congenital absence or severe restriction of horizontal gaze and progressive scoliosis [2,3,4,6]. In non-M3 acute myeloid leukemia, high ROBO3 expression is associated with increased age at diagnosis, poorer risk classification, higher rates of TP53 and RUNX1 mutations, and significantly worse overall and event-free survival. It may also be a biomarker of inferior prognosis [5]. In pancreatic carcinoma, ROBO3 expression is up-regulated, and over-expression promotes cancer cell growth, invasion, and metastasis [7].
In conclusion, Robo3 is essential for the development of commissural circuits in the nervous system. The study of Robo3 through gene-knockout or conditional-knockout mouse models has revealed its role in HGPPS, non-M3 acute myeloid leukemia, and pancreatic carcinoma. Understanding Robo3's function provides insights into the development of the nervous system and the pathogenesis of related diseases, which may contribute to the development of new treatment strategies.
References：
1. Friocourt, François, Chédotal, Alain. 2017. The Robo3 receptor, a key player in the development, evolution, and function of commissural systems. In Developmental neurobiology, 77, 876-890. doi:10.1002/dneu.22478. https://pubmed.ncbi.nlm.nih.gov/28033646/
2. Pinero-Pinto, Elena, Pérez-Cabezas, Verónica, Tous-Rivera, Cristina, Benítez-Lugo, María-Luisa, Sánchez-González, María Carmen. 2020. Mutation in ROBO3 Gene in Patients with Horizontal Gaze Palsy with Progressive Scoliosis Syndrome: A Systematic Review. In International journal of environmental research and public health, 17, . doi:10.3390/ijerph17124467. https://pubmed.ncbi.nlm.nih.gov/32580277/
3. Harahsheh, Ehab Y, Moxley, Lauren E, Al-Amin, Matu, Deniz, Adnan, Osundiji, Mayowa. 2025. 20 years of ROBO3-related horizontal gaze palsy with progressive scoliosis: a mini-review. In Neurogenetics, 26, 30. doi:10.1007/s10048-025-00811-0. https://pubmed.ncbi.nlm.nih.gov/39960500/
4. Yi, Sheng, Qin, Zailong, Zhou, Xunzhao, Chen, Biyan, Luo, Jingsi. 2023. Early onset horizontal gaze palsy and progressive scoliosis due to a noncanonical splicing-site variant and a missense variant in the ROBO3 gene. In Molecular genetics & genomic medicine, 11, e2215. doi:10.1002/mgg3.2215. https://pubmed.ncbi.nlm.nih.gov/37330975/
5. Cai, Zhimei, Wei, Jifeng, Chen, Ze, Wang, Haiqing. 2021. High ROBO3 expression predicts poor survival in non-M3 acute myeloid leukemia. In Experimental biology and medicine (Maywood, N.J.), 246, 1184-1197. doi:10.1177/1535370220988246. https://pubmed.ncbi.nlm.nih.gov/33541130/
6. Sim, Bryan, Ng, Janice Wan Zhen, Sim, Donald Yuhui, Roca, Xavier, Jamuar, Saumya Shekhar. 2023. A novel intronic variant in ROBO3 associated with horizontal gaze palsy with progressive scoliosis: case report and literature review. In Journal of AAPOS : the official publication of the American Association for Pediatric Ophthalmology and Strabismus, 27, 359-363. doi:10.1016/j.jaapos.2023.08.017. https://pubmed.ncbi.nlm.nih.gov/37931836/
7. Han, Shilong, Cao, Chuanwu, Tang, Tao, Zhang, Xiaoping, Li, Maoquan. 2015. ROBO3 promotes growth and metastasis of pancreatic carcinoma. In Cancer letters, 366, 61-70. doi:10.1016/j.canlet.2015.06.004. https://pubmed.ncbi.nlm.nih.gov/26070964/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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