C57BL/6JCya-Dpf3em1flox/Cya
Common Name:
Dpf3-flox
Product ID:
S-CKO-18152
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Dpf3-flox
Strain ID
CKOCMP-70127-Dpf3-B6J-VB
Gene Name
Product ID
S-CKO-18152
Gene Alias
2810403B03Rik; 6530402L11Rik; BAF45C; CERD4; Gm18872; cer-d4
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dpf3em1flox/Cya mice (Catalog S-CKO-18152) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000178756
NCBI RefSeq
NM_001267625
Target Region
Exon 4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
DPF3, a component of the SWI/SNF chromatin remodeling complex, is involved in various biological processes such as cell growth, proliferation, apoptosis, and motility. It is associated with multiple pathways, including TGF-β signaling, and plays a crucial role in maintaining normal cellular functions [2,3,5]. Genetic models, like KO/CKO mouse models, can be valuable for studying its functions.
In dendritic cells, a long non-coding RNA lnc-Dpf3, induced by CCR7 chemokine receptor stimulation, restrains DC migration by inhibiting HIF-1α-mediated glycolysis. DC-specific lnc-Dpf3 deficiency increases CCR7-mediated DC migration, leading to exaggerated adaptive immune responses and inflammatory injuries [1].
In clear cell renal cell carcinoma (ccRCC), the short isoform DPF3a promotes kidney cancer cell migration both in vitro and in vivo. DPF3a interacts with SNIP1, forms a complex with SMAD4 and p300 HAT, and activates cell movement-related genes [2].
In breast cancer, downregulation of DPF3 promotes the proliferation and motility of breast cancer cells through activating JAK2/STAT3 signaling [4]. Also, in renal cancer, germline variation in DPF3 can lead to reduced apoptosis and activation of the STAT3 pathway, and the risk allele at 14q24.2 activates a novel hypoxia-inducible transcription factor-binding enhancer of DPF3 expression, increasing the risk of developing renal cancer [5,6].
In conclusion, DPF3 is essential in regulating cell migration, proliferation, and apoptosis. Studies using KO/CKO mouse models and other loss-of-function experiments have revealed its significant roles in diseases such as cancer and inflammatory diseases. Understanding DPF3's functions provides insights into disease mechanisms and potential therapeutic targets for these conditions.
References:
1. Liu, Juan, Zhang, Xiaomin, Chen, Kun, Li, Zhiqing, Cao, Xuetao. 2019. CCR7 Chemokine Receptor-Inducible lnc-Dpf3 Restrains Dendritic Cell Migration by Inhibiting HIF-1α-Mediated Glycolysis. In Immunity, 50, 600-615.e15. doi:10.1016/j.immuni.2019.01.021. https://pubmed.ncbi.nlm.nih.gov/30824325/
2. Cui, Huanhuan, Yi, Hongyang, Bao, Hongyu, Hu, Yuhui, Chen, Wei. 2022. The SWI/SNF chromatin remodeling factor DPF3 regulates metastasis of ccRCC by modulating TGF-β signaling. In Nature communications, 13, 4680. doi:10.1038/s41467-022-32472-0. https://pubmed.ncbi.nlm.nih.gov/35945219/
3. Druml, Thomas, Brem, Gottfried, Horna, Michaela, Ricard, Anne, Grilz-Seger, Gertrud. 2021. DPF3, A Putative Candidate Gene For Melanoma Etiopathogenesis in Gray Horses. In Journal of equine veterinary science, 108, 103797. doi:10.1016/j.jevs.2021.103797. https://pubmed.ncbi.nlm.nih.gov/34801788/
4. Lin, Wei-Hao, Dai, Wei-Gang, Xu, Xiang-Dong, Li, Jie, Li, He-Ping. 2019. Downregulation of DPF3 promotes the proliferation and motility of breast cancer cells through activating JAK2/STAT3 signaling. In Biochemical and biophysical research communications, 514, 639-644. doi:10.1016/j.bbrc.2019.04.170. https://pubmed.ncbi.nlm.nih.gov/31076105/
5. Colli, Leandro M, Jessop, Lea, Myers, Timothy A, Brown, Kevin M, Chanock, Stephen J. 2021. Altered regulation of DPF3, a member of the SWI/SNF complexes, underlies the 14q24 renal cancer susceptibility locus. In American journal of human genetics, 108, 1590-1610. doi:10.1016/j.ajhg.2021.07.009. https://pubmed.ncbi.nlm.nih.gov/34390653/
6. Protze, Johanna, Naas, Stephanie, Krüger, René, Wullich, Bernd, Schödel, Johannes. 2022. The renal cancer risk allele at 14q24.2 activates a novel hypoxia-inducible transcription factor-binding enhancer of DPF3 expression. In The Journal of biological chemistry, 298, 101699. doi:10.1016/j.jbc.2022.101699. https://pubmed.ncbi.nlm.nih.gov/35148991/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen