C57BL/6JCya-Mmp1aem1flox/Cya
Common Name:
Mmp1a-flox
Product ID:
S-CKO-18155
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mmp1a-flox
Strain ID
CKOCMP-83995-Mmp1a-B6J-VA
Gene Name
Product ID
S-CKO-18155
Gene Alias
MMP-1a; Mcol-A; Mcola
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mmp1aem1flox/Cya mice (Catalog S-CKO-18155) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000217651
NCBI RefSeq
NM_032006
Target Region
Exon 2~4
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Mmp1a, the murine ortholog of human Matrix Metalloproteinase 1 (MMP1), is a key enzyme involved in extracellular matrix (ECM) degradation and remodeling. It is associated with various biological processes such as tumorigenesis, inflammation, and tissue repair, and is thought to signal through protease-activated receptor-1 (PAR1) [1,3,4]. The discovery of Mmp1a in 2001 provided a valuable tool for in vivo studies of MMP1-mediated processes in mice [1].
Mmp1a-deficient mice have been instrumental in understanding its functions. In lung cancer models, Mmp1a deficiency leads to significantly decreased tumor growth and angiogenesis [3]. Co-implantation of lung cancer cells with wild-type Mmp1a(+/+) fibroblasts can restore tumor growth in Mmp1a-deficient animals, highlighting the critical role of stromal-derived Mmp1a [3]. Also, in atherosclerosis models, deficiency of Mmp1a suppresses the development of atherosclerosis [2]. In contrast, in intervertebral disc degeneration, injection of recombinant MMP1a can induce mild to moderate degeneration of the intervertebral discs (IVDs) [5].
In conclusion, Mmp1a plays essential roles in tumorigenesis, angiogenesis, and atherosclerosis development as revealed by gene knockout mouse models. These models have provided insights into its functions in these disease areas, helping to understand the underlying mechanisms and potentially guiding the development of MMP-targeted therapeutics.
References:
1. Foley, Caitlin J, Kuliopulos, Athan. . Mouse matrix metalloprotease-1a (Mmp1a) gives new insight into MMP function. In Journal of cellular physiology, 229, 1875-80. doi:10.1002/jcp.24650. https://pubmed.ncbi.nlm.nih.gov/24737602/
2. Fletcher, Elizabeth K, Wang, Yanling, Flynn, Laura K, Covic, Lidija, Kuliopulos, Athan. 2021. Deficiency of MMP1a (Matrix Metalloprotease 1a) Collagenase Suppresses Development of Atherosclerosis in Mice: Translational Implications for Human Coronary Artery Disease. In Arteriosclerosis, thrombosis, and vascular biology, 41, e265-e279. doi:10.1161/ATVBAHA.120.315837. https://pubmed.ncbi.nlm.nih.gov/33761760/
3. Foley, C J, Fanjul-Fernández, M, Bohm, A, López-Otín, C, Kuliopulos, A. 2013. Matrix metalloprotease 1a deficiency suppresses tumor growth and angiogenesis. In Oncogene, 33, 2264-72. doi:10.1038/onc.2013.157. https://pubmed.ncbi.nlm.nih.gov/23708660/
4. Foley, Caitlin J, Luo, Chi, O'Callaghan, Katie, Covic, Lidija, Kuliopulos, Athan. 2012. Matrix metalloprotease-1a promotes tumorigenesis and metastasis. In The Journal of biological chemistry, 287, 24330-8. doi:10.1074/jbc.M112.356303. https://pubmed.ncbi.nlm.nih.gov/22573325/
5. Lei, Yu, Zhan, Enyu, Chen, Chao, Li, Xingguo, Zhang, Fan. 2024. ALKBH5-mediated m6A demethylation of Runx2 mRNA promotes extracellular matrix degradation and intervertebral disc degeneration. In Cell & bioscience, 14, 79. doi:10.1186/s13578-024-01264-y. https://pubmed.ncbi.nlm.nih.gov/38877576/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen