C57BL/6JCya-Jag2em1flox/Cya
Common Name
Jag2-flox
Product ID
S-CKO-18160
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-16450-Jag2-B6J-VB
Status
When using this mouse strain in a publication, please cite “Jag2-flox Mouse (Catalog S-CKO-18160) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Jag2-flox
Strain ID
CKOCMP-16450-Jag2-B6J-VB
Gene Name
Product ID
S-CKO-18160
Gene Alias
sm, Serh, D12Ggc2e, mJagged2-1
Background
C57BL/6JCya
Gene Full Name
jagged 2
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 12 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000075827
NCBI Transcript ID
NM_010588
Target Region
Exon 1~2
Size of Effective Region
~2.3 kb
Overview of Gene Research
Jag2, short for jagged canonical Notch ligand 2, is a key ligand in the Notch signaling pathway. The Notch signaling pathway is involved in multiple cellular processes such as cell fate determination, proliferation, and apoptosis, which are crucial for embryonic development and tissue homeostasis [4,5].
In non-small cell lung cancer (NSCLC), deletion of Jag2 in cancer cells (a form of loss-of-function experiment) attenuated tumor growth and activated anti-tumor T cell responses. Jag2-deficient lung tumors had macrophages expressing immunostimulatory mediators, suggesting that Jag2 ablation promotes Nr4a-mediated induction of Notch ligands DLL1/4 on cancer cells, which in turn activates macrophage immunostimulatory functionality via Notch1/2 signaling [1].
In intervertebral disc degeneration (IVDD), recombinant JAG2 induced Notch2 and Hes1/Hey2 expression, promoting nucleus pulposus (NP) cell proliferation. Down-regulation of Notch2/Hes1/Hey2 led to G0/G1 phase cell cycle arrest in NP cells. Intradiscal injection of JAG2 alleviated IVDD in a rat model, indicating that JAG2/Notch2 inhibits IVDD by modulating cell proliferation, apoptosis, and extracellular matrix [2].
In ovarian cancer, JAG2+ tumor-associated neutrophils (TANs) enhanced Notch signaling activation, promoting the differentiation of naïve CD4+ T cells into effector regulatory T cells (eTregs), which hampers PD-1 blockade response. Pharmacological targeting of Notch signaling or JAG2 neutralization antibodies enhanced the efficacy of PD-1 monoclonal antibodies in xenograft and patient-derived tumor organoid models [3].
In summary, Jag2, through the Notch signaling pathway, plays important roles in various biological processes and disease conditions. Gene knockout or conditional knockout models, such as those in NSCLC, IVDD, and ovarian cancer, have revealed its functions in tumor growth regulation, cell proliferation, apoptosis, and immune evasion, providing insights into potential therapeutic strategies for these diseases.
References：
1. Mandula, Jay K, Sierra-Mondragon, Rosa A, Jimenez, Rachel V, Conejo-Garcia, Jose R, Rodriguez, Paulo C. 2024. Jagged2 targeting in lung cancer activates anti-tumor immunity via Notch-induced functional reprogramming of tumor-associated macrophages. In Immunity, 57, 1124-1140.e9. doi:10.1016/j.immuni.2024.03.020. https://pubmed.ncbi.nlm.nih.gov/38636522/
2. Long, Jun, Wang, Xiaobo, Du, Xianfa, Li, Xudong, Zheng, Zhaomin. 2019. JAG2/Notch2 inhibits intervertebral disc degeneration by modulating cell proliferation, apoptosis, and extracellular matrix. In Arthritis research & therapy, 21, 213. doi:10.1186/s13075-019-1990-z. https://pubmed.ncbi.nlm.nih.gov/31619270/
3. Wang, Chenyang, Yang, Moran, Zhong, Yujing, Huang, Yan, Liu, Haiou. 2025. Immunosuppressive JAG2+ tumor-associated neutrophils hamper PD-1 blockade response in ovarian cancer by mediating the differentiation of effector regulatory T cells. In Cancer communications (London, England), , . doi:10.1002/cac2.70021. https://pubmed.ncbi.nlm.nih.gov/40120139/
4. Reynolds, Susan D, Hill, Cynthia L, Alsudayri, Alfahdah, Chiang, Tendy, Cormet-Boyaka, Estelle. 2022. Assemblies of JAG1 and JAG2 determine tracheobronchial cell fate in mucosecretory lung disease. In JCI insight, 7, . doi:10.1172/jci.insight.157380. https://pubmed.ncbi.nlm.nih.gov/35819850/
5. Katoh, Masuko, Katoh, Masaru. 2019. Precision medicine for human cancers with Notch signaling dysregulation (Review). In International journal of molecular medicine, 45, 279-297. doi:10.3892/ijmm.2019.4418. https://pubmed.ncbi.nlm.nih.gov/31894255/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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