C57BL/6JCya-Psmc3ipem1flox/Cya
Common Name
Psmc3ip-flox
Product ID
S-CKO-18172
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-19183-Psmc3ip-B6J-VB
Status
When using this mouse strain in a publication, please cite “Psmc3ip-flox Mouse (Catalog S-CKO-18172) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Psmc3ip-flox
Strain ID
CKOCMP-19183-Psmc3ip-B6J-VB
Gene Name
Product ID
S-CKO-18172
Gene Alias
HOP2, GT198, Tbpip
Background
C57BL/6JCya
Gene Full Name
proteasome (prosome, macropain) 26S subunit, ATPase 3, interacting protein
Modification
Conditional knockout
NCBI ID
Phenotype
Chromosome
Chr 11 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000019447
NCBI Transcript ID
NM_008949
Target Region
Exon 4~8
Size of Effective Region
~2.2 kb
Overview of Gene Research
Psmc3ip, also known as GT198 or Hop2, is a gene with multiple functions. It encodes the nuclear PSMC3-interacting protein, which functions as a co-activator of steroid hormone-mediated gene expression. It is involved in the homologous recombination process during DNA repair of double-strand breaks, particularly in the formation of meiotic D-loop before DNA strand exchange [1,6]. It also participates in pathways related to ovarian development and is potentially important for normal ovarian function [2].
In mitotic cells, depletion of Psmc3ip causes sensitivity to poly (ADP-Ribose) polymerase inhibitors (PARPi) used in cancer treatment and ionizing radiation. Psmc3ip-depleted cells accumulate toxic RAD51 foci in response to DNA damage, show impaired homology-directed DNA repair, and become PARPi sensitive, even in cells lacking both BRCA1 and TP53BP1 [1]. In hepatocellular carcinoma (HCC), knockdown of Psmc3ip blunts cell proliferation, colony formation, and xenografted tumor development, and enhances apoptosis [3]. Mutations in Psmc3ip are associated with premature ovarian insufficiency (POI), with different biallelic mutations reported in patients with primary or secondary amenorrhea [4,5].
In conclusion, Psmc3ip plays essential roles in DNA repair, especially in homologous recombination, and in ovarian function. Studies involving its depletion or mutation have revealed its significance in cancer treatment response and ovarian-related diseases, highlighting its potential as a therapeutic target in HCC and a genetic factor in POI [1,3,4,5].
References：
1. Zelceski, Anabel, Francica, Paola, Lingg, Lea, Rottenberg, Sven, Lord, Christopher J. 2023. MND1 and PSMC3IP control PARP inhibitor sensitivity in mitotic cells. In Cell reports, 42, 112484. doi:10.1016/j.celrep.2023.112484. https://pubmed.ncbi.nlm.nih.gov/37163373/
2. França, Monica Malheiros, Mendonca, Berenice Bilharinho. 2021. Genetics of ovarian insufficiency and defects of folliculogenesis. In Best practice & research. Clinical endocrinology & metabolism, 36, 101594. doi:10.1016/j.beem.2021.101594. https://pubmed.ncbi.nlm.nih.gov/34794894/
3. Ding, Jingli, Li, Yang, Fan, Huxiong, Yang, Juesheng, Zhou, Jianliang. 2018. Knockdown of PSMC3IP suppresses the proliferation and xenografted tumorigenesis of hepatocellular carcinoma cell. In Journal of cellular biochemistry, 120, 5449-5458. doi:10.1002/jcb.27824. https://pubmed.ncbi.nlm.nih.gov/30362169/
4. Sirchia, Fabio, Giorgio, Elisa, Cucinella, Laura, Valente, Enza Maria, Nappi, Rossella E. 2022. Biallelic mutations in PSMC3IP are associated with secondary amenorrhea: expanding the spectrum of premature ovarian insufficiency. In Journal of assisted reproduction and genetics, 39, 1177-1181. doi:10.1007/s10815-022-02471-7. https://pubmed.ncbi.nlm.nih.gov/35352317/
5. Mei, Libin, Huang, Lingling, Huang, Yanru, Su, Zhiying, Li, Ping. 2021. Two novel biallelic mutations in PSMC3IP in a patient affected by premature ovarian insufficiency. In Molecular medicine reports, 25, . doi:10.3892/mmr.2021.12561. https://pubmed.ncbi.nlm.nih.gov/34878148/
6. Schubert, Stephanie, Ripperger, Tim, Rood, Melanie, Schlegelberger, Brigitte, Steinemann, Doris. . GT198 (PSMC3IP) germline variants in early-onset breast cancer patients from hereditary breast and ovarian cancer families. In Genes & cancer, 8, 472-483. doi:10.18632/genesandcancer.132. https://pubmed.ncbi.nlm.nih.gov/28435519/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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