C57BL/6JCya-Lamc3em1flox/Cya
Common Name:
Lamc3-flox
Product ID:
S-CKO-18175
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lamc3-flox
Strain ID
CKOCMP-23928-Lamc3-B6J-VB
Gene Name
Product ID
S-CKO-18175
Gene Alias
1110064A23Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lamc3em1flox/Cya mice (Catalog S-CKO-18175) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028187
NCBI RefSeq
NM_011836
Target Region
Exon 2
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Lamc3, encoding laminin γ3 chain, is involved in providing structural support and participating in cellular processes through protein-containing complex binding and signaling receptor binding. The proteins it interacts with are mainly located in the basement membrane and extracellular matrix component. The gene is potentially associated with pathways like extracellular matrix-receptor interaction and PI3K-Akt signaling pathway [1].
Recessive or complex heterozygous variants of the LAMC3 gene can cause occipital cortical malformation (OCCM), characterized by polymicrogyria and pachygyria of the occipital lobes and childhood-onset seizures. Functional experiments have confirmed that certain variant sites lead to premature truncation of the laminin γ3 chain [1]. In ovarian cancer, low expression of LAMC3 is related to poor prognosis and malignant progression, potentially through regulating oncogene-pathways such as Rap1, MAPK, Ras and cell adhesion-related pathways [2]. In zebrafish embryos, knockdown of Lamc3 impairs motoneuron guidance, indicating its role in this process [3].
In summary, Lamc3 is crucial for structural support and cellular processes, and its variants are associated with various diseases. Research on Lamc3, especially through functional studies like knockdown in zebrafish embryos, helps to understand its role in biological processes and disease mechanisms, such as in cortical malformations and ovarian cancer prognosis [1,2,3].
References:
1. Qian, Xiaohang, Liu, Xiaoying, Zhu, Zeyu, Tang, Huidong, Cao, Li. 2021. Variants in LAMC3 Causes Occipital Cortical Malformation. In Frontiers in genetics, 12, 616761. doi:10.3389/fgene.2021.616761. https://pubmed.ncbi.nlm.nih.gov/34354730/
2. Lei, S M, Liu, X, Xia, L P, Li, L, Yin, F J. . [Relationships between decreased LAMC3 and poor prognosis in ovarian cancer]. In Zhonghua fu chan ke za zhi, 56, 489-497. doi:10.3760/cma.j.cn112141-20210426-00230. https://pubmed.ncbi.nlm.nih.gov/34304441/
3. Eve, Alexander M J, Smith, James C. 2017. Knockdown of Laminin gamma-3 (Lamc3) impairs motoneuron guidance in the zebrafish embryo. In Wellcome open research, 2, 111. doi:10.12688/wellcomeopenres.12394.1. https://pubmed.ncbi.nlm.nih.gov/29417095/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen