C57BL/6JCya-Fbln5em1flox/Cya
Common Name:
Fbln5-flox
Product ID:
S-CKO-18180
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fbln5-flox
Strain ID
CKOCMP-23876-Fbln5-B6J-VB
Gene Name
Product ID
S-CKO-18180
Gene Alias
A55; DANCE; EVEC
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fbln5em1flox/Cya mice (Catalog S-CKO-18180) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000222587
NCBI RefSeq
NM_001361987
Target Region
Exon 3~4
Size of Effective Region
~3.8 kb
Detailed Document
Overview of Gene Research
Fbln5, also known as fibulin 5, is an extracellular matrix (ECM) glycoprotein. It plays crucial roles in maintaining the stability of ECM structures, regulating cell proliferation, and is involved in interactions with components of the basement membrane and extracellular matrix proteins [5,6]. It has been implicated in many biological processes related to vascular and other tissues, and its study via genetic models can provide insights into its functions.
Fbln5 is significantly related to the poor prognosis of gastric cancer (GC) patients, with high FBLN5 mRNA levels associated with a poor prognosis and high expression levels correlated with INFc and N3 lymph node metastasis. It is an independent risk factor for GC patient prognosis [1]. In high-grade serous ovarian carcinoma, decreased FBLN5 expression is associated with unfavorable prognosis, and its overexpression inhibits migration, invasion, and proliferation of ovarian cancer cells [5]. In carotid atherosclerosis, low FBLN5 expression in plaque tissues is related to plaque stability, and it may act mainly through the maintenance of the cellular matrix and reactive oxygen species production [6]. In renal clear cell carcinoma in children and young adults, FBLN5 expression was significantly reduced in tumor tissue compared to normal adjacent tissue, and high expression was associated with a favorable prognosis [4]. Mutations in FBLN5 are related to FBLN5-related cutis laxa syndrome, which is characterized by loose skin, emphysema, and other symptoms [2], and also to demyelinating Charcot-Marie-Tooth neuropathy, with patients having a later age of diagnosis and distinct clinical features [3].
In conclusion, Fbln5 is essential for maintaining the stability of ECM and has a significant impact on multiple biological processes and disease conditions. Its study, especially through gene knockout or conditional knockout models (although not explicitly detailed in provided references), could potentially further clarify its functions in these disease areas such as cancer, connective tissue disorders, and neurological diseases.
References：
1. Bian, Xiulan, Yin, Shengjie, Yin, Xin, Ye, Fei, Zhang, Lei. 2023. Clinical and Biological Significances of FBLN5 in Gastric Cancer. In Cancers, 15, . doi:10.3390/cancers15020553. https://pubmed.ncbi.nlm.nih.gov/36672502/
2. Tekmenuray-Unal, Aysel, Durmaz, Ceren Damla. 2022. FBLN5-Related Cutis Laxa Syndrome: A Case with a Novel Variant and Review of the Literature. In Molecular syndromology, 14, 80-87. doi:10.1159/000525215. https://pubmed.ncbi.nlm.nih.gov/36777703/
3. Safka Brozkova, D, Stojkovic, T, Haberlová, J, Seeman, P, Auer-Grumbach, M. 2020. Demyelinating Charcot-Marie-Tooth neuropathy associated with FBLN5 mutations. In European journal of neurology, 27, 2568-2574. doi:10.1111/ene.14463. https://pubmed.ncbi.nlm.nih.gov/32757322/
4. Zhang, Ming, Chen, Feng, Feng, Shaoguang, Zhu, Dongsheng, Zhu, Zhitao. 2024. FBLN5 as One Presumably Prognostic Gene Potentially Modulating Tumor Immune Microenvironment for Renal Clear Cell Carcinoma in Children and Young Adults. In Pharmacogenomics and personalized medicine, 17, 27-40. doi:10.2147/PGPM.S442803. https://pubmed.ncbi.nlm.nih.gov/38264064/
5. Li, Rongrong, Wu, Huan, Jiang, Huiyang, Yang, Ning, Kong, Beihua. 2020. FBLN5 is targeted by microRNA‑27a‑3p and suppresses tumorigenesis and progression in high‑grade serous ovarian carcinoma. In Oncology reports, 44, 2143-2151. doi:10.3892/or.2020.7749. https://pubmed.ncbi.nlm.nih.gov/32901854/
6. Zheng, Lin, Yue, Xinyang, Li, Minhui, Chen, Ruihan, Dong, Honglin. 2022. Contribution of FBLN5 to Unstable Plaques in Carotid Atherosclerosis via mir128 and mir532-3p Based on Bioinformatics Prediction and Validation. In Frontiers in genetics, 13, 821650. doi:10.3389/fgene.2022.821650. https://pubmed.ncbi.nlm.nih.gov/35356421/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen