C57BL/6JCya-Nrgnem1flox/Cya
Common Name:
Nrgn-flox
Product ID:
S-CKO-18181
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Nrgn-flox
Strain ID
CKOCMP-64011-Nrgn-B6J-VB
Gene Name
Product ID
S-CKO-18181
Gene Alias
0710001B06Rik; NG; NG/RC3; Pss1; RC3
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Nrgnem1flox/Cya mice (Catalog S-CKO-18181) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000065668
NCBI RefSeq
NM_022029
Target Region
Exon 2~3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Nrgn, or neurogranin, is a small brain protein expressed in various telencephalic areas. It plays an essential role in synaptic plasticity by regulating the availability of calmodulin (CaM) [4].
In glioma, NRGN acts as a tumor suppressor. LINC00641 can upregulate NRGN by absorbing miR-4262, thus inhibiting glioma cell proliferation [1]. Also, in glioblastoma, lncRNA RBPMS-AS1 promotes NRGN transcription through the miR-301a-3p/CAMTA1 axis, enhancing the radiosensitivity of glioblastoma [5].
In patients with mild traumatic brain injury, serum NRGN levels are significantly higher in those with traumatic intracranial lesions compared to those without, suggesting its potential as a diagnostic biomarker [2]. Moreover, a single-nucleotide polymorphism (rs12807809) in the NRGN gene is associated with abnormal Papez circuit function in schizophrenia patients [3]. Additionally, in the Han Chinese population, polymorphisms in NRGN are associated with schizophrenia, major depressive disorder, and bipolar disorder [8].
In Alzheimer's disease, NRGN levels in neuron-derived extracellular vesicles in plasma are elevated, potentially serving as a biomarker [7]. In adults with obstructive sleep apnea without dementia, higher levels of NRGN in neuron-derived exosomes are associated with cognitive impairment [6].
In conclusion, Nrgn is crucial for synaptic plasticity. Its study in various disease models has revealed its significance in glioma, traumatic brain injury, schizophrenia, mental disorders, Alzheimer's disease, and obstructive sleep apnea-related cognitive impairment. These findings contribute to understanding the disease mechanisms and may offer new diagnostic and therapeutic targets.
References：
1. Yang, Jinghui, Yu, Duo, Liu, Xueshibojie, Changyong, E, Yu, Shan. 2020. LINC00641/miR-4262/NRGN axis confines cell proliferation in glioma. In Cancer biology & therapy, 21, 758-766. doi:10.1080/15384047.2020.1776581. https://pubmed.ncbi.nlm.nih.gov/32543324/
2. Çevik, Serdar, Özgenç, Mustafa Murat, Güneyk, Ahmet, Hanımoğlu, Hakan, Kaynar, Mehmet Yaşar. 2019. NRGN, S100B and GFAP levels are significantly increased in patients with structural lesions resulting from mild traumatic brain injuries. In Clinical neurology and neurosurgery, 183, 105380. doi:10.1016/j.clineuro.2019.105380. https://pubmed.ncbi.nlm.nih.gov/31234132/
3. Zhang, Yifan, Gong, Xiaohong, Yin, Zhiyang, Wang, Fei, Tang, Yanqing. 2019. Association between NRGN gene polymorphism and resting-state hippocampal functional connectivity in schizophrenia. In BMC psychiatry, 19, 108. doi:10.1186/s12888-019-2088-5. https://pubmed.ncbi.nlm.nih.gov/30953482/
4. Rueda-García, Valentina, Rondón-Barragán, Iang Schroniltgen. 2023. Molecular Characterization of Neurogranin (NRGN) Gene from Red‑Bellied Pacu (Piaractus brachypomus). In Molecular neurobiology, 61, 2620-2630. doi:10.1007/s12035-023-03700-5. https://pubmed.ncbi.nlm.nih.gov/37922064/
5. Li, Wenyang, Cui, Yan, Ma, Wenjia, Cai, Yang, Jiang, Yugang. 2021. LncRNA RBPMS-AS1 promotes NRGN transcription to enhance the radiosensitivity of glioblastoma through the microRNA-301a-3p/CAMTA1 axis. In Translational oncology, 15, 101282. doi:10.1016/j.tranon.2021.101282. https://pubmed.ncbi.nlm.nih.gov/34800915/
6. Li, Mengfan, Shen, Tengqun, Yao, Ran, Li, Zhenguang, Zhang, Jinbiao. 2024. Mitochondrial dysfunction is associated with cognitive impairment in adults with OSA without dementia. In Sleep medicine, 119, 234-243. doi:10.1016/j.sleep.2024.04.035. https://pubmed.ncbi.nlm.nih.gov/38704871/
7. Eitan, Erez, Thornton-Wells, Tricia, Elgart, Katya, Smith, Daniel G, Kapogiannis, Dimitrios. 2023. Synaptic proteins in neuron-derived extracellular vesicles as biomarkers for Alzheimer's disease: novel methodology and clinical proof of concept. In Extracellular vesicles and circulating nucleic acids, 4, 133-150. doi:10.20517/evcna.2023.13. https://pubmed.ncbi.nlm.nih.gov/37842184/
8. Wen, Zujia, Chen, Jianhua, Khan, Raja Amjad Waheed, Li, Wenjin, Shi, Yongyong. 2016. Polymorphisms in NRGN are associated with schizophrenia, major depressive disorder and bipolar disorder in the Han Chinese population. In Journal of affective disorders, 194, 180-7. doi:10.1016/j.jad.2016.01.034. https://pubmed.ncbi.nlm.nih.gov/26828755/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen