C57BL/6JCya-Dgkzem1flox/Cya
Common Name:
Dgkz-flox
Product ID:
S-CKO-18206
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dgkz-flox
Strain ID
CKOCMP-104418-Dgkz-B6J-VB
Gene Name
Product ID
S-CKO-18206
Gene Alias
E130307B02Rik; F730209L11Rik; mDGK[z]
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dgkzem1flox/Cya mice (Catalog S-CKO-18206) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000028667
NCBI RefSeq
NM_138306
Target Region
Exon 2~4
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Dgkz, also known as Diacylglycerol kinase ζ, is a diacylglycerol kinase that metabolizes diacylglycerol to yield phosphatidic acid. It is involved in lipid signaling pathways which are crucial for neural activities, memory, and learning [3]. It has been associated with various biological processes and diseases, with its role being explored through different genetic models.
In triple-negative breast cancer, knockout of Dgkz in cell lines significantly inhibited metastatic behaviors in vitro and in vivo, indicating its role as a prometastatic gene by promoting the TGFβ signaling pathway through suppressing lipid raft-dependent endocytosis of TGFβR2 [1]. In osteosarcoma, silence of Dgkz by shRNA hampered cell growth and promoted apoptosis in vitro, and its knockout suppressed xenograft tumor proliferation in vivo, suggesting it acts as an oncogene potentially interacting with the ERK1/2 and MYC pathway [2]. Similar tumor-suppressing effects of Dgkz knockdown were seen in cervical cancer and acute myeloid leukemia, with impacts on cell proliferation, apoptosis, and cell cycle arrest [4,5].
In conclusion, Dgkz plays significant roles in cancer progression, including in triple-negative breast cancer, osteosarcoma, cervical cancer, and acute myeloid leukemia. Knockout and knockdown studies in cell lines and in vivo models have revealed its oncogenic potential in promoting tumor cell growth, metastasis, and survival, highlighting its importance as a potential therapeutic target in these cancer types.
References:
1. Zhao, Yuanyuan, Sun, Hefen, Li, Xuan, Hou, Yifeng, Jin, Wei. 2022. DGKZ promotes TGFβ signaling pathway and metastasis in triple-negative breast cancer by suppressing lipid raft-dependent endocytosis of TGFβR2. In Cell death & disease, 13, 105. doi:10.1038/s41419-022-04537-x. https://pubmed.ncbi.nlm.nih.gov/35115500/
2. Yu, Wenxi, Tang, Lina, Lin, Feng, Yao, Yang, Shen, Zan. 2019. DGKZ Acts as a Potential Oncogene in Osteosarcoma Proliferation Through Its Possible Interaction With ERK1/2 and MYC Pathway. In Frontiers in oncology, 8, 655. doi:10.3389/fonc.2018.00655. https://pubmed.ncbi.nlm.nih.gov/30662872/
3. Alinaghi, Somayeh, Alehabib, Elham, Johari, Amir Hossein, Darvish, Hossein, Ghaedi, Hamid. 2019. Expression analysis and genotyping of DGKZ: a GWAS-derived risk gene for schizophrenia. In Molecular biology reports, 46, 4105-4111. doi:10.1007/s11033-019-04860-1. https://pubmed.ncbi.nlm.nih.gov/31087244/
4. Liu, Keying, Xue, Biyun, Bai, Guiqin, Zhang, Wentao. . Downregulation of Diacylglycerol kinase zeta (DGKZ) suppresses tumorigenesis and progression of cervical cancer by facilitating cell apoptosis and cell cycle arrest. In Bioengineered, 12, 1517-1529. doi:10.1080/21655979.2021.1918505. https://pubmed.ncbi.nlm.nih.gov/33926342/
5. Li, Hong, Dong, Changhu, Tian, Yanning, Bai, Yingying, Chao, Xu. . Knockdown of diacylglycerol kinase zeta (DGKZ) induces apoptosis and G2/M phase arrest in human acute myeloid leukemia HL-60 cells through MAPK/survivin/caspase pathway. In Die Pharmazie, 74, 418-422. doi:10.1691/ph.2019.9386. https://pubmed.ncbi.nlm.nih.gov/31288898/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen