C57BL/6JCya-Jmjd7em1flox/Cya
Common Name:
Jmjd7-flox
Product ID:
S-CKO-18225
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Jmjd7-flox
Strain ID
CKOCMP-433466-Jmjd7-B6J-VA
Gene Name
Product ID
S-CKO-18225
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Jmjd7em1flox/Cya mice (Catalog S-CKO-18225) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000044675
NCBI RefSeq
NM_001114637
Target Region
Exon 2~8
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Jmjd7, or Jumonji-C (JmjC) domain-containing 7, is a 2-oxoglutarate (2OG)-dependent oxygenase. It is involved in multiple functions such as (3S)-lysyl hydroxylation of TRAFAC GTPases like DRG1 and DRG2 [2]. It may also be coupled with the arginine methylation activities of arginine methyltransferases, playing roles in transcription regulation and the biogenesis of membrane-less organelles [1].
In breast cancer cells, depletion of JMJD7 greatly decreases cell proliferation, indicating its role in cancer-related cell growth [4]. In osteoclast differentiation, Jmjd7 acts as a negative regulator. Knockdown of Jmjd7 in RAW 264.7 cells and BMMs enhanced osteoclast differentiation, increased the expression of osteoclast-related genes, and boosted bone resorptive functions [3]. In head and neck squamous cell carcinoma (HNSCC), the read-through fusion gene JMJD7-PLA2G4B promotes cell proliferation and survival. Ablation of JMJD7-PLA2G4B significantly inhibited HNSCC cell proliferation and increased starvation-induced cell death [5].
In conclusion, Jmjd7 plays crucial roles in various biological processes and disease conditions. Its functions in cell proliferation, as seen in breast cancer and HNSCC, and in osteoclast differentiation highlight its significance. These findings from different cellular models contribute to understanding the biological functions of Jmjd7 and its potential as a therapeutic target in related diseases.
References：
1. Liu, Haolin, Wei, Pengcheng, Zhang, Qianqian, Liu, Junfeng, Zhang, Gongyi. 2022. The Novel Protease Activities of JMJD5-JMJD6-JMJD7 and Arginine Methylation Activities of Arginine Methyltransferases Are Likely Coupled. In Biomolecules, 12, . doi:10.3390/biom12030347. https://pubmed.ncbi.nlm.nih.gov/35327545/
2. Markolovic, Suzana, Zhuang, Qinqin, Wilkins, Sarah E, Coleman, Mathew L, Schofield, Christopher J. 2018. The Jumonji-C oxygenase JMJD7 catalyzes (3S)-lysyl hydroxylation of TRAFAC GTPases. In Nature chemical biology, 14, 688-695. doi:10.1038/s41589-018-0071-y. https://pubmed.ncbi.nlm.nih.gov/29915238/
3. Liu, Yingci, Arai, Atsushi, Kim, Terresa, Park, No-Hee, Kim, Reuben H. . Histone Demethylase Jmjd7 Negatively Regulates Differentiation of Osteoclast. In The Chinese journal of dental research, 21, 113-118. doi:10.3290/j.cjdr.a40437. https://pubmed.ncbi.nlm.nih.gov/29808174/
4. Liu, Haolin, Wang, Chao, Lee, Schuyler, Hagman, James, Zhang, Gongyi. 2017. Clipping of arginine-methylated histone tails by JMJD5 and JMJD7. In Proceedings of the National Academy of Sciences of the United States of America, 114, E7717-E7726. doi:10.1073/pnas.1706831114. https://pubmed.ncbi.nlm.nih.gov/28847961/
5. Cheng, Yingduan, Wang, Yi, Li, Jiong, Chang, Insoon, Wang, Cun-Yu. . A novel read-through transcript JMJD7-PLA2G4B regulates head and neck squamous cell carcinoma cell proliferation and survival. In Oncotarget, 8, 1972-1982. doi:10.18632/oncotarget.14081. https://pubmed.ncbi.nlm.nih.gov/28030848/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen