C57BL/6JCya-Trrapem1flox/Cya
Common Name:
Trrap-flox
Product ID:
S-CKO-18232
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Trrap-flox
Strain ID
CKOCMP-100683-Trrap-B6J-VB
Gene Name
Product ID
S-CKO-18232
Gene Alias
-
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Trrapem1flox/Cya mice (Catalog S-CKO-18232) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000100467
NCBI RefSeq
NM_001081362
Target Region
Exon 9~10
Size of Effective Region
~2.2 kb
Detailed Document
Overview of Gene Research
TRRAP, short for transformation/transcription domain-associated protein, is a key adaptor protein and a component of histone acetyltransferase (HAT) complexes. Structurally, it belongs to the phosphoinositide 3-kinase-related kinases (PIKK) family but is a pseudokinase. It functions in gene transcription regulation by acting as a scaffold for multiple transcription factors and HAT complexes, being involved in processes like cell cycle progression, stemness maintenance, and neural homeostasis [1].
In zebrafish, trrap gene-knockout led to smaller eyes, heads, reduced ventral pharyngeal arch size, and impaired tooth mineralization, with changes in dlx3 and dlx2b expression, suggesting its role in craniofacial development [3]. In mouse models, Trrap deletion in Purkinje neurons caused neurodegeneration in old mice, and TRRAP was found to be required for SP1-mediated regulation of microtubule dynamics [4]. In colon cancer cells, TRRAP knockdown decreased the expression of a cancer stem cell marker and spheroid-forming ability, and enhanced the expression of a tumor suppressor gene, indicating its role in tumorigenicity [2].
In conclusion, TRRAP is essential for gene transcription regulation through its role in HAT complex assembly and interaction with transcription factors. Model-based research, especially gene-knockout models in zebrafish and mice, has revealed its importance in craniofacial development, neurodegeneration prevention, and tumorigenesis, providing insights into related disease mechanisms.
References:
1. Yin, Bo-Kun, Wang, Zhao-Qi. 2021. Beyond HAT Adaptor: TRRAP Liaisons with Sp1-Mediated Transcription. In International journal of molecular sciences, 22, . doi:10.3390/ijms222212445. https://pubmed.ncbi.nlm.nih.gov/34830324/
2. Kang, Kyung-Taek, Shin, Min-Joo, Moon, Hye-Ji, Suh, Dong-Soo, Kim, Jae-Ho. 2023. TRRAP Enhances Cancer Stem Cell Characteristics by Regulating NANOG Protein Stability in Colon Cancer Cells. In International journal of molecular sciences, 24, . doi:10.3390/ijms24076260. https://pubmed.ncbi.nlm.nih.gov/37047234/
3. Suzuki, Taichi, Hirai, Yo, Uehara, Tomoko, Kosaki, Kenjiro, Kawahara, Atsuo. 2021. Involvement of the zebrafish trrap gene in craniofacial development. In Scientific reports, 11, 24166. doi:10.1038/s41598-021-03123-z. https://pubmed.ncbi.nlm.nih.gov/34934055/
4. Tapias, Alicia, Lázaro, David, Yin, Bo-Kun, Neri, Francesco, Wang, Zhao-Qi. 2021. HAT cofactor TRRAP modulates microtubule dynamics via SP1 signaling to prevent neurodegeneration. In eLife, 10, . doi:10.7554/eLife.61531. https://pubmed.ncbi.nlm.nih.gov/33594975/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen