C57BL/6NCya-Msl3em1flox/Cya
Common Name:
Msl3-flox
Product ID:
S-CKO-18234
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Msl3-flox
Strain ID
CKOCMP-17692-Msl3-B6N-VA
Gene Name
Product ID
S-CKO-18234
Gene Alias
Msl31; Msl3l1
Background
C57BL/6NCya
NCBI ID
Modification
Conditional knockout
Chromosome
X
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Msl3em1flox/Cya mice (Catalog S-CKO-18234) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000033725
NCBI RefSeq
NM_001370791
Target Region
Exon 2~4
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
Msl3, a member of the chromatin-associated male-specific lethal MSL complex, is conserved from flies to humans. In Drosophila, it is crucial for the transcriptional upregulation of genes on the male X chromosome for dosage compensation [4].
In female Drosophila oogenesis, Msl3, acting independently of the rest of the MSL complex, promotes germline stem cell (GSC) differentiation. It does so by regulating the transcription of genes like RpS19b, which is required for the translation of Rbfox1, a meiotic cell cycle entry factor [1].
In mice, studies using a male germline-specific Stra8-iCre driver and a Msl3flox conditional knock-out (CKO) mouse line were conducted. Conditional loss-of-function of Msl3 in spermatogonia did not cause spermatogenesis defects or changes in meiosis-related gene expression. This indicates that in mice, Msl3 has a different expression pattern compared to Drosophila and primates, and loss-of-function mutations in its chromodomain alone do not impede meiotic entry [2,3].
In conclusion, Msl3 plays important roles in Drosophila GSC differentiation and dosage compensation. In mammals, despite conservation of the gene, its function in meiotic entry seems to differ from that in flies and primates as shown by the Msl3 CKO mouse model. In humans, de novo mutations in Msl3 cause an X-linked syndrome marked by impaired histone H4 lysine 16 acetylation, highlighting its significance in human development [5].
References:
1. McCarthy, Alicia, Sarkar, Kahini, Martin, Elliot T, Buszczak, Michael, Rangan, Prashanth. 2022. Msl3 promotes germline stem cell differentiation in female Drosophila. In Development (Cambridge, England), 149, . doi:10.1242/dev.199625. https://pubmed.ncbi.nlm.nih.gov/34878097/
2. Mitchell, Tyler, Lin, Jennifer M, James, Joushua R, Rangan, Prashanth, Forni, Paolo E. 2023. Loss Of Chromodomain of Male-Specific Lethal 3 (MSL3) Does Not Affect Spermatogenesis In Rodents. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.03.16.532933. https://pubmed.ncbi.nlm.nih.gov/36993289/
3. Mitchell, T A, Lin, J M, Hicks, S M, Rangan, P, Forni, P E. 2023. Loss of function of male-specific lethal 3 (Msl3) does not affect spermatogenesis in rodents. In Developmental dynamics : an official publication of the American Association of Anatomists, 253, 453-466. doi:10.1002/dvdy.669. https://pubmed.ncbi.nlm.nih.gov/37847071/
4. Sural, Tuba H, Peng, Shouyong, Li, Bing, Park, Peter J, Kuroda, Mitzi I. 2008. The MSL3 chromodomain directs a key targeting step for dosage compensation of the Drosophila melanogaster X chromosome. In Nature structural & molecular biology, 15, 1318-25. doi:10.1038/nsmb.1520. https://pubmed.ncbi.nlm.nih.gov/19029895/
5. Basilicata, M Felicia, Bruel, Ange-Line, Semplicio, Giuseppe, Thevenon, Julien, Akhtar, Asifa. 2018. De novo mutations in MSL3 cause an X-linked syndrome marked by impaired histone H4 lysine 16 acetylation. In Nature genetics, 50, 1442-1451. doi:10.1038/s41588-018-0220-y. https://pubmed.ncbi.nlm.nih.gov/30224647/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen