C57BL/6JCya-Cemipem1flox/Cya
Common Name
Cemip-flox
Product ID
S-CKO-18235
Backgroud
C57BL/6JCya
Strain ID
CKOCMP-80982-Cemip-B6J-VB
When using this mouse strain in a publication, please cite “Cemip-flox Mouse (Catalog S-CKO-18235) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cemip-flox
Strain ID
CKOCMP-80982-Cemip-B6J-VB
Gene Name
Product ID
S-CKO-18235
Gene Alias
12H19.01.T7, 6330404C01Rik, 9930013L23Rik, Hybid, Kiaa1199
Background
C57BL/6JCya
NCBI ID
Modification
Conditional knockout
Chromosome
Chr 7
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000064174
NCBI RefSeq
NM_030728
Target Region
Exon 3~5
Size of Effective Region
~2.8 kb
Overview of Gene Research
Cemip, also known as KIAA1199 and HYBID, is a member of the hyaluronidase family. It degrades hyaluronic acid (HA) in the extracellular matrix and remodels it, and is involved in regulating several signaling pathways such as WNT and EGFR [2,4,5,6]. Cemip plays a crucial role in various biological processes and diseases, including tumor initiation and progression, arthritis, atherosclerosis, and idiopathic pulmonary fibrosis [3,4]. Genetic models like KO/CKO mouse models can be valuable for further exploring its functions.
In tumor-related studies, Cemip overexpression is significantly associated with poor overall survival (OS) and disease-free survival (DFS) in cancer patients. It is related to advanced clinical stage, lymph node metastasis, and poor histological grade in multiple cancers [1]. In small-cell lung cancer (SCLC), Cemip-mediated HA degradation activates the TLR2/c-Src/ERK1/2 axis, promoting SCLC metastasis, and Cemip depletion attenuated metastasis in SCLC xenografts [7].
In cardiac remodeling post-myocardial infarction, Cemip promotes TSP4 degradation in an ACTN4-dependent manner, activating the FAK signaling pathway, and Cemip knockdown in mice reduced cardiac fibrosis and improved cardiac function [8].
In conclusion, Cemip is essential in extracellular matrix remodeling through HA degradation and is involved in multiple disease-related biological processes. KO/CKO mouse models have provided insights into its role in cancer metastasis and cardiac remodeling post-myocardial infarction, helping to understand disease mechanisms and potentially identify new therapeutic targets.
References：
1. Chen, Huan, Wang, Qingting, Liu, Jin, Qiu, Yuanjie, Li, Manxiang. 2023. CEMIP as a prognostic biomarker for cancers: a meta- and bioinformatic analysis. In Expert review of molecular diagnostics, 22, 1107-1115. doi:10.1080/14737159.2022.2168191. https://pubmed.ncbi.nlm.nih.gov/36631437/
2. Liu, Yang, Hu, Gang, Li, Yuetong, Bi, Changlong, Zhai, Aixia. 2023. Research on the biological mechanism and potential application of CEMIP. In Frontiers in immunology, 14, 1222425. doi:10.3389/fimmu.2023.1222425. https://pubmed.ncbi.nlm.nih.gov/37662915/
3. Spataro, Sofia, Guerra, Concetta, Cavalli, Andrea, Moll, Solange, Prunotto, Marco. 2022. CEMIP (HYBID, KIAA1199): structure, function and expression in health and disease. In The FEBS journal, 290, 3946-3962. doi:10.1111/febs.16600. https://pubmed.ncbi.nlm.nih.gov/35997767/
4. Domanegg, Kevin, Sleeman, Jonathan P, Schmaus, Anja. 2022. CEMIP, a Promising Biomarker That Promotes the Progression and Metastasis of Colorectal and Other Types of Cancer. In Cancers, 14, . doi:10.3390/cancers14205093. https://pubmed.ncbi.nlm.nih.gov/36291875/
5. Chen, Yu, Zhou, Hu, Jiang, Wen-Jing, Jiang, Yan, Xia, Bai-Rong. 2021. The role of CEMIP in tumors: An update based on cellular and molecular insights. In Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 146, 112504. doi:10.1016/j.biopha.2021.112504. https://pubmed.ncbi.nlm.nih.gov/34922110/
6. Guo, Song, Guo, Yunfei, Chen, Yuanyuan, Zhang, Chunmei, Chen, Dahu. 2024. The role of CEMIP in cancers and its transcriptional and post-transcriptional regulation. In PeerJ, 12, e16930. doi:10.7717/peerj.16930. https://pubmed.ncbi.nlm.nih.gov/38390387/
7. Li, Li, Shen, Xiaoju, Mo, Xiaoxiang, Mao, Naiquan, Yang, Jie. 2023. CEMIP-mediated hyaluronan metabolism facilitates SCLC metastasis by activating TLR2/c-Src/ERK1/2 axis. In Biochimica et biophysica acta. Molecular cell research, 1870, 119451. doi:10.1016/j.bbamcr.2023.119451. https://pubmed.ncbi.nlm.nih.gov/36931608/
8. Zha, Yafang, Luo, Xueyang, Ge, Zhuowang, Li, Yanyan, Zhang, Song. 2024. KIAA1199/CEMIP knockdown attenuates cardiac remodeling post myocardial infarction by activating TSP4 pathway in mice. In Biochimica et biophysica acta. Molecular basis of disease, 1870, 167473. doi:10.1016/j.bbadis.2024.167473. https://pubmed.ncbi.nlm.nih.gov/39173890/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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